PITPNB Mouse mAb - 一次抗体, specific to PITPNB

Cat. No.: Ab121957

GRADE & PURITY KO Validation, ExactAb™, High Performance, See COA

Application

IHC, WB

Host species

マウス

Reactive species

マウス, 人間, 助言

Isotype

Mouse IgG1

Clone Number

OTI5D11

Conjugation

Unconjugated

概要

Mouse anti Human PITPNB Antibody, Monoclonal (OTI5D11), could be used for WB, IHC and so on.

Specifications

Product Name

PITPNB Mouse mAb - 一次抗体, specific to PITPNB

同義語

OTTHUMP00000198846 antibody | OTTHUMP00000198847 antibody | Phosphatidylinositol transfer protein beta isoform antibody | Phosphatidylinositol transfer protein, betaantibody | PI TP beta antibody | PI-TP-beta antibody | PIPNB_HUMAN antibody | Pitpnb antib

仕様と純度

KO Validation, ExactAb™, High Performance, See COA

生化学的・生理学的メカニズム

Catalyzes the transfer of PtdIns and phosphatidylcholine between membranes.

宿主種

マウス

特異性

PITPNB

免疫原

Recombinant full length protein of human PITPNB.

ポジティブコントロール

WB: HEK293, A431 and RAW 264.7 cell lysates. IHC: Human kidney carcinoma, endometrium adenocarcinoma tissues.

活用

Unconjugated

等級

ExactAb™, High Performance, KO Validation

SDS-PAGE

150 kDa

精製方法

Protein G purified

集中

See COA

保管と配送

形状

Liquid

保管条件

Store at -20°C,Avoid repeated freezing and thawing

入荷

Ice chest + Ice pads

安定性と保管

Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

画像

Western Blot: PITPNB Mouse mAb (Ab121957)

PITPNB Mouse mAb (Ab121957) - Western Blot
All lanes: PITPNB Mouse mAb (Ab121957) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 31 kDa
Observed band size: 32 kDa
Exposure time: 64.0 s

Western Blot: PITPNB Mouse mAb (Ab121957)

PITPNB Mouse mAb (Ab121957) - Western Blot
Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells and PITPNB-Knockout HeLa cells were separated by SDS - PAGE and immunoblotted with PITPNB Mouse mAb (Ab121957) (1/500). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody as a loading control.

IHC: PITPNB Mouse mAb (Ab121957)

PITPNB Mouse mAb (Ab121957) - IHC
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using PITPNB Mouse mAb (Ab121957) . (Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120°C for 3 min, Ab121957) (1/2000).

IHC: PITPNB Mouse mAb (Ab121957)

PITPNB Mouse mAb (Ab121957) - IHC
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using PITPNB Mouse mAb (Ab121957) . (Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120°C for 3 min, Ab121957) (1/2000).

適用＃テキヨウ＃

• 適用＃テキヨウ＃: Dilution info
• WB:

  1/500-1/2000;

• IHC:

  1/2000;

関連ターゲット（人間）

PITPNB Tbio Phosphatidylinositol transfer protein beta isoform (0 Activities)

Discover Target: PITPNB

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

よくある質問

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does KO Validation mean?

KO Validation indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (PITPNB Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.