Rab20 Mouse mAb - 一次抗体, specific to Rab20, Mouse IgG2a

Cat. No.: Ab124145

GRADE & PURITY Carrier Free, Validated, ExactAb™, High Performance, 0.5 mg/mL

Application

Flow Cytometry, WB

Host species

マウス

Reactive species

マウス, 人間

Isotype

Mouse IgG2a

Clone Number

1694CT210.218.54

Conjugation

Unconjugated

概要

Mouse anti Human Rab20 Antibody, Monoclonal (1694CT210.218.54), could be used for WB, Flow Cytometry and so on.

Specifications

Product Name

Rab20 Mouse mAb - 一次抗体, specific to Rab20, Mouse IgG2a

同義語

FLJ20429 antibody | RAB20 antibody | RAB20 member RAS oncogene family antibody | RAB20_HUMAN antibody | Ras related protein Rab20 antibody | Ras-related protein Rab-20 antibody

仕様と純度

Carrier Free, ExactAb™, Validated, High Performance, 0.5 mg/mL

生化学的・生理学的メカニズム

Plays a role in apical endocytosis/recycling. Plays a role in the maturation and acidification of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis. Plays a role in the fusion of phagosomes with lysosomes.

宿主種

マウス

特異性

Rab20

免疫原

Recombinant protein of human RAB20 (AA 1-190)

ポジティブコントロール

WB: THP-1, K562 and MCF7 cell lysates. Flow: HeLa cells.

活用

Unconjugated

等級

Carrier Free, ExactAb™, High Performance, Validated

アイソタイプ

Mouse IgG2a

SDS-PAGE

150 kDa

精製方法

Protein G purified

集中

0.5 mg/mL

保管と配送

形状

Liquid

保管条件

Store at -20°C,Avoid repeated freezing and thawing

入荷

Ice chest + Ice pads

安定性と保管

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

画像

Western Blot: Rab20 Mouse mAb (Ab124145)

Rab20 Mouse mAb (Ab124145) - Western Blot
All lanes: Rab20 Mouse mAb (Ab124145) at 1/500 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti Mouse HRP (Ab179001) at 1/10000 dilution

Predicted band size: 26 kDa
Observed band size: 29 kDa
Exposure time: 60.0 s

Flow Cytometry: Rab20 Mouse mAb (Ab124145)

Rab20 Mouse mAb (Ab124145) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Rab20 Mouse mAb (Ab124145) (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by Rab20 Mouse mAb (Ab124145) (1/25 dilution) for 60 min at 37 ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37 ºC. Isotype control antibody (blue line) was mouse IgG2a (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

適用＃テキヨウ＃

• 適用＃テキヨウ＃: Dilution info
• Flow Cytometry:

  1/25;

• WB:

  1/500-1/4000;

関連ターゲット（人間）

RAB20 Tbio Ras-related protein Rab-20 (0 Activities)

Discover Target: RAB20

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)