GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.See COA
Recombinant, ExactAb™, Validated, See COA ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
概要
Rabbit anti Human CDKN2A/p14ARF Antibody, Recombinant, could be used for WB, ICC, IF and so on. Application: WB: 1/500-1/1000 ICC/IF: 1/50-1/200 Relevance The gene for CDK2NA generates several transcripts/proteins which differ from each other in their first exons. Three of these transcripts are generated by alternative splicing (isoform 1 a.k.a p16INK4A, isoform 2 and isoform 3 a.k.a p12), two of which are known to function as inhibitors of CDK4 kinase. One other transcript that is generated from this gene contains an alternate reading frame (ARF), with the first exon located 20kb upstream of the remainder of the gene(isoform 4 a.k.a. p14ARF, p19ARF, ARF). In spite of the structural and some functional differences, all the proteins encoded by the CDKN2A gene are involved in cell cycle G1 control.
Specifications
Product Name
Recombinant CDKN2A/p14ARF Antibody - 一次抗体, specific to CDKN2A, Rabbit IgG
A synthetic peptide derived from human CDKN2A/p14ARF (AA 75-132).
ポジティブコントロール
WB: HEK293 and LNCaP cell lysates. IF/ICC: HeLa cells.
活用
Unconjugated
等級
ExactAb™, Recombinant, Validated
アイソタイプ
Rabbit IgG
ライトチェーン・タイプ
kappa
SDS-PAGE
150 kDa
精製方法
Protein A purified
集中
See COA
保管と配送
形状
Liquid
保管条件
Store at -20°C,Avoid repeated freezing and thawing
入荷
Ice chest + Ice pads
安定性と保管
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
画像
Recombinant CDKN2A/p14ARF Antibody (Ab095833) - Western Blot All lanes: Recombinant CDKN2A/p14ARF Antibody (Ab095833) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution Predicted band size: 14 kDa Observed band size: 16 kDa Exposure time: 100.0 s
Recombinant CDKN2A/p14ARF Antibody (Ab095833) - IF/ICC Immunofluorescent analysis of HeLa cells, using Recombinant CDKN2A/p14ARF Antibody (Ab095833) at 1/50 dilution.
適用＃テキヨウ＃
適用＃テキヨウ＃
Dilution info
IF/ICC
1/50-1/200
WB
1/500-1/1000
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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