Recombinant E2F2 Antibody - 一次抗体, specific to E2F2, Rabbit IgG

Cat. No.: Ab100785

グレード＆純度 Recombinant, ExactAb™, Validated, See COA

Application

WB

Host species

ウサギ

Reactive species

マウス, 人間, 助言

Isotype

Rabbit IgG

Conjugation

Unconjugated

概要

Rabbit anti Human E2F2 Antibody, Recombinant, could be used for WB and so on.
Application:
WB: 1/1000-1/2000
Protein Function
Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from g1 to s phase. E2F-2 binds specifically to RB1 protein, in a cell-cycle dependent manner.

仕様

Product Name

Recombinant E2F2 Antibody - 一次抗体, specific to E2F2, Rabbit IgG

同義語

E2F transcription factor 2 | E2F2 | E2F-2 | E2F-2transcription factor E2F2 | dE2F2 antibody | E2F transcription factor 2 antibody | E2F-2 antibody | E2F2 antibody | E2F2_HUMAN antibody | Transcription factor E2F2 antibody | E2F2 | E2F transcription factor

仕様と純度

Recombinant, ExactAb™, Validated, See COA

宿主種

ウサギ

特異性

E2F2

免疫原

A synthetic peptide derived from human E2F2 (AA 313-375)

ポジティブコントロール

WB: Jurkat, HeLa, Hep G2 and C6 cell lysates.

活用

Unconjugated

等級

ExactAb™, Recombinant, Validated

アイソタイプ

Rabbit IgG

ライトチェーン・タイプ

kappa

SDS-PAGE

150 kDa

精製方法

Protein A purified

集中

See COA

Product Line

生物製品

保管と配送

形状

Liquid

保管条件

Store at -20°C,Avoid repeated freezing and thawing

入荷

Ice chest + Ice pads

安定性と保管

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

画像

Western Blot: Recombinant E2F2 Antibody (Ab100785)

Recombinant E2F2 Antibody (Ab100785) - Western Blot
All lanes: Recombinant E2F2 Antibody (Ab100785) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution

Predicted band size: 47 kDa
Observed band size: 51 kDa
Exposure time: 120.0 s

適用＃テキヨウ＃

適用＃テキヨウ＃

Dilution info

WB

1/500-1/2000

関連ターゲット（人間）

E2F2 Tbio Transcription factor E2F2 (0 Activities)

Discover Target: E2F2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

よくある質問

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant E2F2 Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.