グレード&純度Recombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.See COA
Rabbit anti Human E2F6 Antibody, Recombinant, could be used for WB and so on. Application: WB: 1/500-1/2000 Protein Function Inhibitor of E2F-dependent transcription. Binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Has a preference for the 5'-TTTCCCGC-3' E2F recognition site. E2F-6 lacks the transcriptional activation and pocket protein binding domains. Appears to regulate a subset of E2F-dependent genes whose products are required for entry into the cell cycle but not for normal cell cycle progression. May silence expression via the recruitment of a chromatin remodeling complex containing histone H3-K9 methyltransferase activity. Overexpression delays the exit of cells from the S-phase.
仕様
Product Name
Recombinant E2F6 Antibody - 一次抗体, specific to E2F6, Rabbit IgG
同義語
E2F binding site modulating activity protein | E2F transcription factor 6 | E2F transcription factor 6 isoform 1 | EMA | MGC111545 | Transcription factor E2F6 | E2F6_HUMAN | E2F 6 antibody | E2F 6 | E2F binding site modulating activity protein antibody |
仕様と純度
Recombinant, ExactAb™, Validated, See COA
宿主種
ウサギ
特異性
E2F6
免疫原
A synthetic peptide derived from human E2F6 (AA 100-281)
ポジティブコントロール
WB: K562, Jurkat, Hep G2 and HeLa cell lysates.
活用
Unconjugated
等級
ExactAb™, Recombinant, Validated
アイソタイプ
Rabbit IgG
ライトチェーン・タイプ
kappa
SDS-PAGE
150 kDa
精製方法
Protein A purified
集中
See COA
Product Line
生物製品
保管と配送
形状
Liquid
保管条件
Store at -20°C,Avoid repeated freezing and thawing
入荷
Ice chest + Ice pads
安定性と保管
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
画像
Recombinant E2F6 Antibody (Ab100799) - Western Blot All lanes: Recombinant E2F6 Antibody (Ab100799) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 32 kDa Observed band size: 32 kDa Exposure time: 100.0 s
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レビュー
顧客レビュー
よくある質問
What does ExactAb? mean?
ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.
What does Recombinant mean?
Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.
How should this product be stored?
Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.
How is this product shipped?
This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.
What documentation is provided?
Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.
Which species reactivity has this antibody been validated against?
Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant E2F6 Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.
Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.