Recombinant eIF4E Antibody - 一次抗体, specific to EIF4E, Rabbit IgG

Cat. No.: Ab101380

GRADE & PURITY Recombinant, Validated, ExactAb™, 0.5 mg/mL

Application

IF/ICC, IHC, WB

Host species

ウサギ

Reactive species

マウス, 人間, 助言

Isotype

Rabbit IgG

Conjugation

Unconjugated

概要

Rabbit anti Human eIF4E Antibody, Recombinant, could be used for WB, IHC and so on.

Application:

WB: 1/500-1/1000

IHC: 1/50-1/100

IF/ICC: 1/200

Protein Function

Its translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1 (By similarity). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.

Specifications

Product Name

Recombinant eIF4E Antibody - 一次抗体, specific to EIF4E, Rabbit IgG

同義語

CBP | eIF4E | eIF-4E | EIF4E1 | EIF4EL1 | EIF4EL1MGC111573 | eIF-4F 25 kDa subunit | EIF4F | eukaryotic translation initiation factor 4E | eukaryotic translation initiation factor 4E-like 1 | mRNA cap-binding protein | AUTS19 antibody | CBP antibody | eIF

仕様と純度

ExactAb™, Validated, Recombinant, 0.5 mg/mL

宿主種

ウサギ

特異性

EIF4E

免疫原

A synthetic peptide derived from human eIF4E (AA 176-194)

ポジティブコントロール

WB: HeLa, K562, A431, HEK293, C6 and NIH/3T3 lysates. IHC: Mouse colon tissue. IF/ICC: HeLa cells.

活用

Unconjugated

等級

ExactAb™, Recombinant, Validated

アイソタイプ

Rabbit IgG

SDS-PAGE

150 kDa

精製方法

Immunogen affinity purified

集中

0.5 mg/mL

保管と配送

形状

Liquid

保管条件

Store at -20°C,Avoid repeated freezing and thawing

入荷

Ice chest + Ice pads

安定性と保管

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

画像

Western Blot: Recombinant eIF4E Antibody (Ab101380)

Recombinant eIF4E Antibody (Ab101380) - Western Blot
All lanes: Recombinant eIF4E Antibody (Ab101380) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 25 kDa
Observed band size: 27 kDa
Exposure time: 21.2 s

IHC: Recombinant eIF4E Antibody (Ab101380)

Recombinant eIF4E Antibody (Ab101380) - IHC
Immunohistochemistry analysis of paraffin-embedded mouse colon using Recombinant eIF4E Antibody (Ab101380). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

IF/ICC: Recombinant eIF4E Antibody (Ab101380)

Recombinant eIF4E Antibody (Ab101380) - IF/ICC

IF analysis of Recombinant eIF4E Antibody (Ab101380) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant eIF4E Antibody (Ab101380) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).

適用＃テキヨウ＃

• 適用＃テキヨウ＃: Dilution info
• WB:

  1/500-1/1000

• IHC:

  1/50 - 1/100;

• IF/ICC:

  1/200;

関連ターゲット（人間）

EIF4E Tchem Eukaryotic translation initiation factor 4E (0 Activities)

Discover Target: EIF4E

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

EIF4E Tchem Eukaryotic translation initation factor (600 Activities)

Discover Target: EIF4E

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

EIF4E Tchem Eukaryotic translation initiation factor 4E/Eukaryotic translation initiation factor 4E-binding protein 1 (15 Activities)

Discover Target: EIF4E

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

EIF4E Tchem EIF4E/EIF4G1 (7 Activities)

Discover Target: EIF4E

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

作用メカニズム

よくある質問

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant eIF4E Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.