Recombinant Human Complement Factor D Protein

Cat. No.: rp181599

GRADE & PURITY Animal Free, Carrier Free, His Tag, ≥95%(SDS-PAGE), expressed in HEK293; See COA

Accession #

P00746

Protein Tag

C-10His

Expression system

HEK293

Endotoxin Concentration

<1.0 EU/μg

Bioactivity

Testing in progress

Specifications

Product Name

Recombinant Human Complement Factor D Protein

同義語

CFD | Adipsin | PFD | DF | Complement factor D | C3 convertase activator | Properdin factor D | ADN | Complement Factor D (Adipsin) | D Component Of Complement (Adipsin) | EC 3.4.21.46 | AND | Complement Factor D Preproprotein | EC 3.4.21

等級

Animal Free, Carrier Free, His Tag

仕様と純度

Animal Free, Carrier Free, His Tag, ≥95%(SDS-PAGE), expressed in HEK293; See COA

生化学的・生理学的メカニズム

Complement Factor D is a serine protease that catalyzes the initial proteolytic step in the alternative pathway of complement. Expressed in adipose tissue at high levels, factor D is also known as adipsin. It is an exceptionally specific protease and the

生物活性

Testing in progress

エンドトキシン濃度

<1.0 EU/μg

アミノ酸

1-253 aa

シーケンス

MHSWERLAVLVLLGAAACAAPPRGRILGGREAEAHARPYMASVQLNGAHLCGGVLVAEQWVLSAAHCLEDAADGKVQVLLGAHSLSQPEPSKRLYDVLRAVPHPDSQPDTIDHDLLLLQLSEKATLGPAVRPLPWQRVDRDVAPGTLCDVAGWGIVNHAGRRPDSLQHVLLPVLDRATCNRRTHHDGAITERLMCAESNRRDSCKGDSGGPLVCGGVLEGVVTSGSRVCGNRKKPGIYTRVASYAAWIDSVLAHHHHHHHHHH

アクセス番号

P00746

予想分子量

28.4 kDa

SDS-PAGE

27.3 kDa, under reducing conditions; 26.7 kDa, under non-reducing conditions.

分子タイプ

タンパク質

Product Line

生物製品

保管と配送

集中

expressed in HEK293; See COA

再構成

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

保管条件

Store at -20°C,Avoid repeated freezing and thawing

入荷

Ice chest + Ice pads

安定性と保管

Store at -20℃ for 12 months. Upon reconstitution, it is recommended to aliquot. Avoid freeze/thaw cycle.

画像

SDS-PAGE: Recombinant Human Complement Factor D Protein (rp181599)

Recombinant Human Complement Factor D Protein (rp181599) - SDS-PAGE 

3 μg/lane of Recombinant Human Complement Factor D Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 27.3 kDa under reducing conditions and 26.7 kDa under non-reducing conditions. In the absence of reducing agents, intramolecular disulfide bonds lock Complement Factor D into a compact conformation, leading to retarded migration and a dimer-like apparent molecular weight in SDS-PAGE (Uniport: P00746).

よくある質問

What is the purity of this product, and how is it determined?

This product is supplied at ≥95% purity, determined by SDS-PAGE. Lot-specific values are stated on the Certificate of Analysis.

What does Animal Free mean?

Animal Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.