グレード＆純度Carrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Bioactive?Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure.ActiBioPure™?ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.PBS Only?PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers.≥95%(SDS-PAGE)
Accession #
P01579
Protein Tag
No tag
Expression system
E. coli
Endotoxin Concentration
<1.0 EU/μg
Bioactivity
1. Recombinant human IFN-gamma bioactivity is measured by inhibition of the proliferation of HT-29 cells.The EC50 for this effect is typically 1.41 ng/mL. 2. Immobilized Recombinant Human IFN-gamma protein (rp186559) at 1.0 μg/mL can bind Fontolizumab (anti-IFNG) (Ab175539) with the EC50 of 23.33 ng/mL.
IFN gamma, also known as IFNG, is a secreted protein that belongs to the type II interferon family. IFN gamma is produced predominantly by natural killer and natural killer T cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymp
生物活性
1. Recombinant human IFN-gamma bioactivity is measured by inhibition of the proliferation of HT-29 cells.The EC50 for this effect is typically 1.41 ng/mL. 2. Immobilized Recombinant Human IFN-gamma protein (rp186559) at 1.0 μg/mL can bind Fontolizumab (anti-IFNG) (Ab175539) with the EC50 of 23.33 ng/mL.
14.1 kDa, under reducing conditions; 14.1 kDa, under non-reducing conditions
分子タイプ
タンパク質
Product Line
生物製品
保管と配送
再構成
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
保管条件
Store at -20°C,Avoid repeated freezing and thawing
入荷
Ice chest + Ice pads
安定性と保管
Store at -20°C for 1 year. Avoid freeze / thaw cycle.
画像
Recombinant Human IFN-gamma Protein (rp186559) - Protein Bioactivity Recombinant Human IFN-gamma bioactivity is measured by inhibition of the proliferation of HT-29 cells. The EC50 for this effect is typically 1.41 ng/mL.
Recombinant Human IFN-gamma Protein (rp186559) - ELISA Immobilized Recombinant Human IFN-gamma protein (rp186559) at 1.0 μg/mL can bind Fontolizumab (anti-IFNG) (Ab175539) with the EC50 of 23.33 ng/mL.
Recombinant Human IFN-gamma Protein (rp186559) - SDS-PAGE 3 μg/lane of Recombinant Human IFN-gamma Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 14.1 kDa.
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レビュー
顧客レビュー
よくある質問
What does Carrier Free mean?
Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.
What does Bioactive mean?
Bioactive indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.
How should this product be stored?
Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.
How is this product shipped?
This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.
What documentation is provided?
Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.
What is the purity of this product, and how is it determined?
This product is supplied at ≥95% purity, determined by SDS-PAGE. Lot-specific values are stated on the Certificate of Analysis.
Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
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