グレード＆純度Animal Free?Animal-free — produced without animal-derived components to reduce contamination risk. Use in biomanufacturing and culture avoiding animal-origin material.Carrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Bioactive?Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure.ActiBioPure™?ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.His Tag?His tag grade — recombinant protein bearing a His tag for affinity purification/detection. Use to purify, immobilize, or detect the tagged protein.≥95%(SDS-PAGE)
Accession #
P01027
Protein Tag
C-His
Expression system
E. coli
Endotoxin Concentration
<1.0 EU/μg
Bioactivity
Measured by its ability to enhance IL-6 secretion by human peripheral blood mononuclear cells (PBMC). The ED50 for this effect is typically 0.8-4 µg/mL
Purity ≥95% (SDS-PAGE) Endotoxin level <1.0 EU/μg Function C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates. Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
仕様
Product Name
Recombinant Mouse Complement Component C3a Protein
同義語
Acylation stimulating protein cleavage product | AHUS5 | ARMD9 | ASP | C3 | C3 and PZP like alpha 2 macroglobulin domain containing protein 1 | C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1 | c3 complement | C3adesArg | CO3_HUMAN | Com
等級
ActiBioPure™, Animal Free, Azide Free, Bioactive, Carrier Free, High Performance, His Tag
仕様と純度
Animal Free, Carrier Free, Bioactive, ActiBioPure™, Azide Free, High Performance, His Tag, ≥95%(SDS-PAGE)
生物活性
Measured by its ability to enhance IL-6 secretion by human peripheral blood mononuclear cells (PBMC). The ED50 for this effect is typically 0.8-4 µg/mL
Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.
保管条件
Store at -20°C,Avoid repeated freezing and thawing
入荷
Ice chest + Ice pads
安定性と保管
Store at -20~-80℃ for more than 1 year. Upon delivery aliquot. Avoid freeze/thaw cycle.
画像
Recombinant Mouse Complement Component C3a Protein (rp153647) - SDS-PAGE
2 μg/lane of Recombinant Mouse Complement Component C3a Protein was resolved with SDS-PAGE under reducing (R) condition and visualized by Coomassie® Blue staining, showing the band at 11 kDa under reducing condition.
Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
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