RFC4 Mouse mAb - 一次抗体, specific to RFC4

Cat. No.: Ab125155

GRADE & PURITY Validated, ExactAb™, High Performance, See COA

Application

IF/ICC, IHC, WB

Host species

マウス

Reactive species

しかし, マウス, 人間, 助言, 猿

Isotype

Mouse IgG1

Clone Number

OTI1A8

Conjugation

Unconjugated

概要

Mouse anti Human RFC4 Antibody, Monoclonal (OTI1A8), could be used for WB, IHC, IF/ICC and so on.

Specifications

Product Name

RFC4 Mouse mAb - 一次抗体, specific to RFC4

同義語

A1 37 antibody | A1 37 kDa subunit antibody | Activator 1 37 antibody | Activator 1 37 kDa subunit antibody | Activator 1 subunit 4 antibody | Replication factor C 37 antibody | Replication factor C 37 kDa subunit antibody | Replication factor C subunit 4

仕様と純度

ExactAb™, Validated, High Performance, See COA

生化学的・生理学的メカニズム

The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. This subunit may be involved in the elongation of the multiprimed DNA templ

宿主種

マウス

特異性

RFC4

免疫原

Recombinant full length protein of human RFC4 (AA 1-363).

ポジティブコントロール

WB: Hep G2, HeLa, A549, Jurkat, MCF7, NIH/3T3 and C6 cell lysates. IHC: Human pancreas, pancreas carcinoma, tonsil tissues. IF/ICC: COS7 cells transiently transfected with pCMV6-ENTRY RFC4.

活用

Unconjugated

等級

ExactAb™, High Performance, Validated

SDS-PAGE

150 kDa

精製方法

Protein G purified

集中

See COA

保管と配送

形状

Liquid

保管条件

Store at -20°C,Avoid repeated freezing and thawing

入荷

Ice chest + Ice pads

安定性と保管

Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

画像

Western Blot: RFC4 Mouse mAb (Ab125155)

RFC4 Mouse mAb (Ab125155) - Western Blot
All lanes: RFC4 Mouse mAb (Ab125155) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 40 kDa
Observed band size: 38 kDa
Exposure time: 61.2 s

IHC: RFC4 Mouse mAb (Ab125155)

RFC4 Mouse mAb (Ab125155) - IHC
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using RFC4 Mouse mAb (Ab125155). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 120°C for 3 min, Ab125155)

IHC: RFC4 Mouse mAb (Ab125155)

RFC4 Mouse mAb (Ab125155) - IHC
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using RFC4 Mouse mAb (Ab125155). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 120°C for 3 min, Ab125155).

IHC: RFC4 Mouse mAb (Ab125155)

RFC4 Mouse mAb (Ab125155) - IHC
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using RFC4 Mouse mAb (Ab125155). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 120°C for 3 min, Ab125155).

IF/ICC: RFC4 Mouse mAb (Ab125155)

RFC4 Mouse mAb (Ab125155) - IF/ICC
RFC4 Mouse mAb (Ab125155) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RFC4.

適用＃テキヨウ＃

• 適用＃テキヨウ＃: Dilution info
• IF/ICC:

  1/100;

• IHC:

  1/150

• WB:

  1/200-1/4000

関連ターゲット（人間）

RFC4 Tbio Replication factor C subunit 4 (0 Activities)

Discover Target: RFC4

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

よくある質問

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (RFC4 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.