S100A10 Mouse mAb - 一次抗体, specific to S100A10, Rabbit IgG

Cat. No.: Ab126219

グレード＆純度 ExactAb™, Validated, 0.5 mg/mL

Application

IF/ICC, WB

Host species

マウス

Reactive species

マウス, 人間, 助言

Isotype

Mouse IgG2a

Clone Number

6F4E6D5C10

Conjugation

Unconjugated

概要

Mouse anti Human S100A10 Antibody, Monoclonal (6F4E6D5C10), could be used for WB, IF/ICC and so on.

仕様

Product Name

S100A10 Mouse mAb - 一次抗体, specific to S100A10, Rabbit IgG

同義語

Calpactin I light chain | Calpactin-1 light chain | Cellular ligand of annexin II | p10 protein | p11 | S100 calcium-binding protein A10 | 42C antibody | AA409961 antibody | AL024248 antibody | Annexin II ligand antibody | Annexin II ligand, calpactin I,

仕様と純度

ExactAb™, Validated, 0.5 mg/mL

生化学的・生理学的メカニズム

Because S100A10 induces the dimerization of ANXA2/p36, it may function as a regulator of protein phosphorylation in that the ANXA2 monomer is the preferred target (in vitro) of tyrosine-specific kinase.

宿主種

マウス

特異性

S100A10

免疫原

Recombinant protein of mouse S100A10.

ポジティブコントロール

WB: HeLa, A431, TH-29, A549, HUVEC and NIH/3T3 cell lysates. IF/ICC: HeLa cells.

活用

Unconjugated

等級

ExactAb™, Validated

アイソタイプ

Rabbit IgG

SDS-PAGE

150 kDa

精製方法

Immunogen affinity purified

集中

0.5 mg/mL

Product Line

生物製品

保管と配送

形状

Liquid

保管条件

Store at -20°C,Avoid repeated freezing and thawing

入荷

Ice chest + Ice pads

安定性と保管

Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

画像

Western Blot: S100A10 Mouse mAb (Ab126219)

S100A10 Mouse mAb (Ab126219) - Western Blot
All lanes: S100A10 Mouse mAb (Ab126219) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 11 kDa
Observed band size: 13 kDa
Exposure time: 58.4 s

IF/ICC: S100A10 Mouse mAb (Ab126219)

S100A10 Mouse mAb (Ab126219) - IF/ICC
Immunofluorescent analysis of S100-A10 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with S100A10 Mouse mAb (Ab126219) in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

適用＃テキヨウ＃

適用＃テキヨウ＃

Dilution info

WB

1/500-1/1000

IF/ICC

1/50-1/100

関連ターゲット（人間）

S100A10 Tbio Protein S100-A10 (0 Activities)

Discover Target: S100A10

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)