Sabouraud Dextrose Broth (EP) - BioReagent

Cat. No.: S1528331

GRADE & PURITY BioReagent

Storage

Room temperature

Shipped In

Normal

概要

For mold and yeast testing. Preparation method: Weigh 30 g of this product in 1 L of distilled or deionized water, heat to boiling until completely dissolved, dispense, autoclave at 121°C for 15 minutes, and set aside for use.

Specifications

仕様と純度

BioReagent

保管条件

Room temperature

入荷

Normal

等級

BioReagent

Product Line

biological_products

Application Protocols

No item-specific, validated protocols are provided in the Product Data.

General literature guidance (not item-specific):

• Growth promotion test (GPT): Inoculate prepared sterile SDB with low inocula (10–100 CFU) of representative fungi (e.g., Candida albicans, Aspergillus brasiliensis). Incubate under organism-appropriate conditions and verify recovery and growth characteristics within specified timeframes per your pharmacopeia/SOP.
• Enrichment prior to plating: Inoculate sample into SDB, incubate to enrich fungal biomass, then streak onto solid media (e.g., Sabouraud Dextrose Agar) for isolation and enumeration.
• Supplementation: After sterilization and cooling, aseptically add antibiotics or acidifying agents if a selective variant is required by the method.

For detailed, validated instructions (medium mass per liter, sterilization cycle, target pH, acceptance criteria), consult your internal methods and the lot-specific CoA/Spec Sheet.

Biological Roles

Sabouraud Dextrose Broth (SDB) is formulated to favor the growth of yeasts and filamentous fungi while inhibiting many bacteria (literature). Its function is ecological selection within mixed microbial communities and robust support of fungal biomass.

• Key formulation principles (literature):
  • High dextrose concentration supplies readily metabolizable carbon for fermentative and oxidative pathways common to fungi (glycolysis → TCA → respiration/fermentation).
  • Peptones provide amino acids, peptides, vitamins, and growth factors, supporting protein synthesis and secondary metabolism.
  • Acidic pH (~5.6) suppresses many bacterial species without unduly inhibiting most fungi.
• Experimental roles:
  • Enrichment medium for low-level fungal contaminants in samples from manufacturing environments, water systems, or consumer products (literature).
  • Maintenance cultures for yeast strains used in biochemical studies (e.g., carbohydrate utilization, stress responses) (literature).
  • Pre-culture growth prior to downstream assays (e.g., susceptibility testing in defined media), ensuring cells are in log phase and physiologically active (literature best practice).

Note: Actual organism recovery depends on strain physiology, inoculum quality, temperature, aeration, and medium lot characteristics. Validate with relevant organisms and follow applicable pharmacopeial or internal method suitability criteria.

Buffer Applications

This product is not a dedicated buffer system. Sabouraud Dextrose Broth provides nutrients and has limited intrinsic buffering capacity.

• Typical pH (literature): ~5.6 at 25 °C after sterilization; pH may drift during growth due to metabolic acid/base production by fungi.
• Guidance:
  • Do not rely on SDB to maintain a fixed pH during experiments requiring stringent pH control. Use a defined buffer system if constant pH is critical.
  • If pH control is desired, consider overlay buffers or use defined media containing appropriate buffering salts, and validate organism performance accordingly.

No specific buffer recipes or applications are associated with this item.

Green Alternatives

While culture media selection is driven primarily by biological performance, sustainability considerations can be incorporated without compromising quality.

• Resource considerations (general):
  • Dehydrated media reduce shipping weight/volume versus ready-to-use liquid, lowering transport emissions.
  • Prepare only the volume needed; unused dehydrated powder stores well at room temperature if kept dry, reducing waste.
• Process energy:
  • Autoclaving is energy-intensive. Where validated and biologically acceptable, sterile filtration of the dissolved broth (without agar) can reduce energy use; confirm that filtration does not alter performance for your application (literature caveat).
• Alternative media (literature comparison):
  • Potato Dextrose Broth (PDB): plant-derived nutrients; slightly different carbon/nitrogen profile, may enhance sporulation of some fungi; not as inhibitory to bacteria as acidified SDB.
  • Malt Extract Broth (MEB): derived from malt; favorable for many molds; similar preparation footprint.
• Sourcing and additives:
  • Prefer peptones derived from sustainable/traceable sources when available; consider plant-based peptones if consistent with your method validation (literature trend).

Trade-offs: Any medium change requires revalidation (growth promotion, recovery, method suitability). Performance must take precedence over marginal environmental gains in regulated testing.

Pharmaceutical Uses

For research and quality control laboratory use only. Not for administration to humans or animals.

• Role in pharmaceutical/biotech QC (literature):
  • Commonly used for growth promotion testing and suitability checks for yeasts and molds in accordance with pharmacopeial microbiology chapters (e.g., EP/USP general microbiological tests), where Sabouraud media are recognized for fungal recovery.
  • Employed as an enrichment medium in bioburden and environmental monitoring programs to detect fungal contamination of manufacturing areas, utilities, and raw materials (method-dependent).
  • Utilized in preservative efficacy/challenge tests as a recovery medium for fungal challenge organisms, per compendial/industry guidance.
• EP designation: “(EP)” indicates alignment with European Pharmacopoeia usage; item-specific certification or compliance claims are Not specified for this item; refer to CoA/Spec Sheet and validate per your SOPs.

Regulatory note: Any use in regulated testing requires documented growth-promotion testing, sterility checks, and method suitability with target organisms per your quality system.

Physical Properties

As a dehydrated culture medium mixture, conventional single-compound properties (bp, mp, logP) are not applicable. Practical properties relevant to use are provided below.

• Appearance (dehydrated): Not specified for this item; refer to CoA/Spec Sheet.
• Solubility: Freely soluble in water (literature, for typical Sabouraud dextrose media components).
• Typical working pH: ~5.6 at 25 °C after sterilization (literature, formulation-dependent; not item-specific).
• Osmolality/ionic strength: Variable; depends on exact formulation and water quality (literature).
• Sterilization: Heat sterilizable by autoclaving (literature – verify against CoA/Spec and your cycle).
• Hygroscopicity: Dehydrated media are moisture-sensitive; protect from humidity to prevent clumping and performance drift (general handling guidance).

Item-specific values such as moisture limits, ash content, pH range, or clarity after sterilization: Not specified for this item; refer to CoA/Spec Sheet.

Note: If preparing concentrated stocks or adding selective supplements, measure and adjust pH only after complete dissolution and before sterilization; final pH should be verified post-sterilization (literature best practice).

Quality and Grades

• Grade: BioReagent — indicates suitability for laboratory biological work, including preparation of culture media and general microbiology workflows. BioReagent-grade media components emphasize low bioburden and consistent performance across lots compared with technical grades.
• EP designation: “(EP)” indicates conformance in intent to European Pharmacopoeia use for Sabouraud media in compendial microbiological procedures (e.g., growth promotion testing of yeasts/molds), subject to verification. Item-specific compliance, including growth-promotion performance, is Not specified for this item; refer to CoA/Spec Sheet.
• What to expect from a quality Sabouraud Dextrose Broth:
  • Lot-to-lot performance consistency (growth promotion within specified ranges) (general expectation)
  • Tight control of raw materials (peptone source, dextrose quality) (general expectation)
  • Defined pH after sterilization and clarity standards (literature/compendial expectations)
• Recommended verifications on receipt (best practice):
  • Growth promotion testing with appropriate control strains (e.g., representative yeasts and molds per pharmacopeial guidance; literature)
  • pH verification at 25 °C post-sterilization
  • Sterility check for uninoculated controls

For definitive release specifications (moisture, pH, growth-promotion criteria, microbial limits), consult the lot-specific CoA/Spec Sheet.

Reaction and Applications

Chemical reaction applications are not applicable. Instead, focus is on microbiological applications.

• Primary use (literature): Cultivation, maintenance, and enumeration of fungi (yeasts and molds) owing to high carbohydrate content and acidic pH that inhibit many bacteria.
• Typical workflows:
  • Environmental/bioburden monitoring (fungal counts) from air, surfaces, or water by enrichment in broth followed by plating (literature)
  • Food, cosmetic, and personal-care product testing for yeast/mold contamination per compendial or industry methods (literature)
  • Antibiotic potency or preservative efficacy studies where fungal challenge organisms are required (literature; medium used as growth matrix)
• Practical tips (literature/best practice):
  • Perform growth promotion testing with representative strains (e.g., Candida spp., Aspergillus spp.) to qualify each lot and preparation.
  • Adjust pH only after full dissolution; verify final pH post-sterilization as autoclaving can shift pH.
  • For selective applications, sterilely add antibiotics/acidifying agents after cooling to 45–50 °C (or room temperature for broth) to avoid thermal degradation.
  • For enumeration, consider serial dilution in a non-nutritive diluent to minimize carryover inhibition.

Note: Specific validated methods and organisms may be mandated by your internal SOPs or pharmacopeial chapters; follow those requirements.

Reaction Conditions

Chemical reaction conditions are not applicable. The relevant “conditions” pertain to cultivation parameters for fungi (general literature guidance; not item-specific):

• Preparation: Dissolve dehydrated SDB completely in purified water with gentle heating; adjust pH if needed; sterilize (e.g., 121 °C for 15 min). Verify final pH after sterilization.
• Incubation temperature: Typically 20–30 °C for molds; 25–37 °C for many yeasts (organism-dependent; consult strain-specific guidance).
• Aeration/agitation: Provide adequate oxygen transfer; use baffled flasks/shaking for yeasts; static or gently aerated conditions for filamentous fungi depending on morphology.
• Inoculum: Standardize inoculum density to achieve reproducible growth curves; use validated reference strains for method suitability.
• Duration: 24–72 h for many yeasts; longer for filamentous fungi (organism- and endpoint-dependent).

These parameters must be optimized and validated for the organisms and assays in your laboratory. Always follow internal SOPs and applicable pharmacopeial or industry standards.

Safety and Handling

• GHS classification, pictograms, hazard and precautionary statements: Not specified for this item; refer to the SDS for authoritative safety information.
• Likely hazards (general for dehydrated media): Low acute toxicity; dust may cause mechanical eye/respiratory irritation. Avoid inhalation, contact with eyes/skin. Implement dust control when weighing.
• PPE: Laboratory coat, safety glasses, appropriate gloves; use a dust mask/respirator if airborne dusts may be generated. Handle in a clean area to maintain medium sterility.
• Incompatibilities: Avoid strong oxidizers and moisture during storage. Do not mix with biocides/antimicrobials unless intended for selectivity tests.
• Microbiological risk: After inoculation, cultures will contain viable microorganisms. Treat all spent media and culture materials as biohazardous; decontaminate by autoclaving before disposal in accordance with institutional biosafety procedures.
• First aid (general):
  • Inhalation: Move to fresh air; seek medical attention if symptoms persist.
  • Eye contact: Rinse cautiously with water for several minutes; remove contact lenses if present and easy to do.
  • Skin contact: Wash with soap and water.
  • Ingestion: Rinse mouth; seek medical advice if unwell.
• Fire safety: Organic components are combustible; use water spray, CO2, foam, or dry chemical as appropriate. Combustion may produce COx and nitrogenous fumes.
• Always defer to the product SDS and your institution’s biosafety guidelines.

Solvent Selection

This product is not a solvent; it is a dehydrated nutrient medium intended for dissolution in water.

• Recommended solvent: Purified water meeting relevant laboratory or pharmacopeial quality (e.g., EP Purified Water or equivalent) to avoid inhibitory ions or bioburden (general best practice).
• Miscibility: The reconstituted broth is an aqueous solution/suspension; miscible with water. Organic co-solvents are not recommended as they may inhibit fungal growth (general guidance).
• Practical notes:
  • Dissolve with gentle heating and stirring to ensure complete solubilization before sterilization (literature).
  • Avoid high hardness or high TOC water; both can affect pH and clarity and may inhibit growth in sensitive assays.
• When to choose Sabouraud Dextrose Broth vs alternatives (literature):
  • Choose SDB for routine cultivation/enumeration of yeasts and molds where an acidic, high-dextrose medium is desired.
  • Consider alternatives such as Malt Extract Broth (rich in maltose and malt extract) for certain molds, or Potato Dextrose Broth for sporulation studies. For bacterial-fungal mixed samples where bacterial suppression is essential, an antibiotic-supplemented Sabouraud variant may be used under appropriate approvals.

Storage and Reconstitution

• Storage conditions (dehydrated): Room temperature (per Product Data). Keep tightly closed in a dry place; protect from humidity and volatile chemicals.
• Shipping: Normal (per Product Data).
• Shelf life: Not specified for this item; refer to CoA/Spec Sheet.

General preparation (literature; not item-specific):

• Reconstitution: Suspend the appropriate mass of dehydrated Sabouraud Dextrose Broth in purified water (commonly formulated to yield pH ~5.6 at 25 °C after sterilization). Heat with stirring until completely dissolved.
• Sterilization: Autoclave at 121 °C for 15 minutes. For heat-labile supplements, sterile-filter the supplement and add aseptically after the broth cools to room temperature.
• Post-sterilization: Verify pH at 25 °C; inspect for clarity and absence of precipitate. If using as a selective medium, add supplements per protocol.
• Storage of prepared broth: Store at 2–8 °C protected from light; use promptly and according to internal expiry dating (literature best practice). Avoid repeated warming/cooling cycles.

Note: Exact reconstitution mass per liter, acceptable pH range, and growth-promotion criteria are Not specified for this item; refer to the lot-specific CoA/Spec Sheet and your laboratory SOPs.

Structure and Identity

Sabouraud Dextrose Broth (EP) is a complex, undefined microbiological growth medium designed for cultivation of fungi (yeasts and molds). It is a mixture (not a single chemical entity), typically comprising enzymatic digests (peptones) and dextrose.

• Item type: complex nutrient medium (dehydrated powder), European Pharmacopoeia (EP)-type formulation
• Molecular formula: Not applicable (mixture)
• Molecular weight: Not applicable (mixture)
• SMILES / InChI / InChIKey: Not applicable (mixture)
• Structural features (descriptive):
  • Contains carbohydrate source (dextrose) to support fermentative/oxidative fungal metabolism (literature)
  • Contains peptones providing amino acids, peptides, and nitrogen (literature)
  • Formulated at acidic pH to suppress most bacteria while favoring fungi (literature, typical final pH ~5.6)

Because this is not a single compound, discrete 2D/3D structural descriptors do not apply. Where detailed composition is required for method validation, refer to the product’s CoA/Spec Sheet and the applicable EP monograph guidance for Sabouraud media.

Synthetic Utility

Not applicable as a chemical building block. Sabouraud Dextrose Broth is a biological culture medium and is not used as a reagent or intermediate in organic synthesis.

• If chemical compatibility is a concern (e.g., when adding test articles to cultures), consider:
  • Solubility of additives in aqueous, mildly acidic media.
  • Potential inhibitory effects of solvents or reactants on fungal growth.
  • The need for vehicle controls when introducing organic co-solvents at low percentages.

For synthetic chemistry applications, refer instead to dedicated reagents and solvents.

Target Specificity

No antibody, enzyme, or molecular target specificity applies to this product.

• Item-specific target/host/clone/isotype: Not applicable.
• Tested organism list or recovery performance: Not specified for this item; refer to CoA/Spec Sheet.

Functionally, Sabouraud Dextrose Broth is broadly supportive of yeasts and molds (literature), but exact performance must be verified with your organisms of interest.