グレード＆純度BioReagent?BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility.Biological Stain?Biological stain grade — dyes characterized for staining cells and tissues. Use in histology and microscopy where staining consistency matters.
Saponification solution is primarily composed of ethanol and strong alkali. Saponification is a type of chemical modification and blocking method. Its principle involves using an alcohol-containing alkaline solution to cleave O-acetyl groups in enzyme-resistant sialic acids. The removal of O-acetyl groups and the formation of hydroxyl groups on the sialic acid side chains restore the reactivity of sialic acids to PAS. Saponification technology is also used to reverse methylation by cleaving the methyl ester bonds formed during the methylation process and reforming carboxyl groups. This reagent is intended for research purposes only and is not suitable for clinical diagnosis or other applications.
Procedure (for reference only)
Operate according to the specific experimental requirements. Typically, treat at room temperature for 30 minutes.
Precautions
Positive control slides are required to verify the effectiveness of the methylation procedure.
For your safety and health, please wear a lab coat and disposable gloves during operation.
After opening, the reagent should be used as soon as possible to avoid affecting subsequent experimental results.
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What does Biological Stain mean?
Biological Stain indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.
What does BioReagent mean?
BioReagent indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.
How should this product be stored?
Store at room temperature.
How is this product shipped?
This product ships under standard ambient conditions. No temperature-controlled packaging is required.
What documentation is provided?
Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.
Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
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