グレード＆純度Carrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.1.0 mg/mL
Mouse anti Human SCAMP5 Antibody, Monoclonal (K06322_12G11), could be used for WB, IHC, IP and so on. Application: WB: 1/1000 IHC: 1/200 IP: 1/100 Protein Function Required for the calcium-dependent exocytosis of signal sequence-containing cytokines such as CCL5. Probably acts in cooperation with the SNARE machinery. May play a role in accumulation of expanded polyglutamine (polyQ) protein huntingtin (HTT) in case of endoplasmic reticulum stress by inhibiting the endocytosis pathway.
仕様
Product Name
SCAMP5 Mouse mAb - 一次抗体, specific to SCAMP5, Mouse IgG1
Store at -80°C,Avoid repeated freezing and thawing
入荷
Dry ice packs + Cold packs
安定性と保管
Store at 2-8 ℃ (3 months). Store at -20 ℃ (12 months). Store at -80 ℃ (36 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
画像
SCAMP5 Mouse mAb (Ab126689) - Western Blot All lanes: SCAMP5 Mouse mAb (Ab126689) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/10000 dilution
Predicted band size: 26 kDa Observed band size: 24 kDa Exposure time: 100.0 s
SCAMP5 Mouse mAb (Ab126689) - IHC IHC analysis of cerebellum tissue by SCAMP5 Mouse mAb (Ab126689). IHC was performed using sections of the formalin-fixed paraffin-embedded cerebellum tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9.0 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with SCAMP5 Mouse mAb (Ab126689) at 5 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control. Result: Cytoplasm of cells in granular layer and in molecular layer are positively stained.
SCAMP5 Mouse mAb (Ab126689) - IP Immunoprecipitation was performed by incubation of 2.5 µg of SCAMP5 Mouse mAb (Ab126689) with mouse brain lysate containing 200 µg of total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. SCAMP5 Mouse mAb (Ab126689) at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG (Fc specific) was used as the secondary antibody. Lane 1: Mouse brain lysate Lane 2: SCAMP5 immunoprecipitated from mouse brain lysate by SCAMP5 Mouse mAb (Ab126689) Lane 3: The same as Lane 2 but Mouse IgG was used as IgG isotype control antibody Result: SCAMP5 Mouse mAb (Ab126689) can immunoprecipitate SCAMP5.
Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
