Product Introduction:
The protein tracer sample loading buffer (2x reduction) is a double concentrated SDS-PAGE gel electrophoresis sample loading buffer with bromophenol blue as the dye. It has the function of precipitating protein samples into the gel pores and indicating the movement position of protein samples in the gel. At the same time, the buffer contains DTT/B-mercaptoethanol and SDS, which can break the disulfide bond within and between the chains of protein molecules, separate each protein subunit from each other, and denature the protein. It is suitable for the preparation and sample loading of reduced SDS-PAGE protein samples.
Precautions：
1. Before adding the sample, thaw it at room temperature or 37-40 ℃ for a few minutes and gently shake well to ensure even mixing of the solution;
2. This reagent contains β - mercaptoethanol. Please wear experimental clothing and disposable gloves when operating;
3. This product is only used for scientific research and cannot be used for human experiments or treatments;
4. For your own safety, please take protective measures such as wearing lab coats and gloves before using the reagents.
Instructions for Use：
1. Reagent preparation
1.1 Mix the protein tracer loading buffer (2 x reduction) with the protein sample in a 1:1 ratio;
2. Protein sample processing
2.1 Heat the protein sample in a boiling water bath for 3-5 minutes;
2.2 After sufficient denaturation of the protein sample, cool it to room temperature and centrifuge at less than 3000 rpm for 30 seconds;
3. Protein loading and electrophoresis
3.1 After centrifugation, take appropriate amount of supernatant with micro sampler and directly add it into the SDS-PAGE gel sampling hole;
3.2 Conduct routine electrophoresis. Generally, stop electrophoresis when the dye reaches 0.5cm-1cm from the bottom of gel.
ZJ25F0420212