Smad3 Mouse mAb - 一次抗体, specific to Smad3, Mouse IgG2b

Cat. No.: Ab188096
注文可能
GRADE & PURITY Carrier Free ? Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. Azide Free ? Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory. Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. High Performance ? High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short. PBS Only ? PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers. ≥95%(SDS-PAGE) 1.0 mg/mL
Application
Flow Cytometry, IF/ICC, WB
Host species
マウス
Reactive species
マウス, 人間, 助言
Isotype
Mouse IgG2b
Clone Number
FG01/2C1
Conjugation
Unconjugated
★
Size
USA
ドイツ (EU)*
Price
Qty
10μl
Ab188096-10μl
在庫あり ≥10
—
$79.90
50μl
Ab188096-50μl
7 在庫あり
—
$259.90
100μl
Ab188096-100μl
2 在庫あり
—
$419.90
1ml
Ab188096-1ml
US製・受注生産 · 2~4週間 ·
—
$2,799.90
Enter a quantity for the sizes you want to add.
🧪
Why this grade

Carrier Free, ExactAb™, Azide Free, Validated, High Performance, PBS Only, ≥95%(SDS-PAGE), 1.0 mg/mL Azide Free,Carrier Free,ExactAb™,High Performance,PBS Only,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

概要

WB: 1/1000 – 1/100,000

Flow: 1/200 - 1/2,000

ICC/IF: 1/200

Specifications

Product Name
Smad3 Mouse mAb - 一次抗体, specific to Smad3, Mouse IgG2b
同義語
DKFZP586N0721 antibody | DKFZp686J10186 antibody | hMAD 3 antibody | hMAD-3 antibody | hSMAD3 antibody | HSPC193 antibody | HST17436 antibody | JV15 2 antibody | JV15-2 antibody | JV152 antibody | LDS1C antibody | LDS3 antibody | MAD (mothers against deca
仕様と純度
Carrier Free, ExactAb™, Azide Free, Validated, High Performance, PBS Only, ≥95%(SDS-PAGE), 1.0 mg/mL
宿主種
マウス
特異性
Smad3
免疫原
Recombinant full length protein corresponding to Human Smad3
ポジティブコントロール
WB: A549, Jurkat, K562, HEK293, HeLa, A431, NIH/3T3 and C6 whole cell lysates; Mouse brain tissue lysate Flow: A549 cells ICC/IF: U-2 OS cells
活用
Unconjugated
等級
Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated
アイソタイプ
Mouse IgG2b
ライトチェーン・タイプ
kappa
SDS-PAGE
150.0 KDa
精製方法
Protein A purified
集中
1.0 mg/mL
Product Line
生物製品
保管と配送
保管条件
Store at -20°C,Avoid repeated freezing and thawing
入荷
Ice chest + Ice pads
安定性と保管
Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
画像

Samd3 Mouse mAb (Ab188096) - Western Blot
All lanes: Samd3 Mouse Antibody (Ab188096) at 1/20000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 48 kDa
Observed band size: 49 kDa
Exposure time: 0.9 s 

Samd3 Mouse mAb (Ab188096) - Western Blot
All lanes: Samd3 Mouse Antibody (Ab188096) at 1/20000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 48 kDa
Observed band size: 48 kDa
Exposure time: 29.6 s 

Samd3 Mouse mAb (Ab188096) - Flow Cytometry
Overlay histogram showing A549 cells stained with Samd3 Mouse mAb (Ab188096) (Red). Cells were fixed and permeabilized with 100% methanol. A Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG (Ab170221) was used as an isotype control (Blue).

Samd3 Mouse mAb (Ab188096) - ICC/IF
IF analysis of Samd3 (Green) in U-2 OS cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Samd3 Mouse mAb (Ab188096) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/500 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

適用#テキヨウ#
適用#テキヨウ#
Dilution info
Flow Cytometry

1/200-1/2000

IF/ICC

1/200

WB

1/1000 – 1/100,000

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

関連ターゲット(人間)
SMAD3 Tchem Mothers against decapentaplegic homolog 3 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
SMAD3 Tchem Mothers against decapentaplegic homolog 3 (68039 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
SMAD3 Tchem Mothers against decapentaplegic homolog 2/homolog 3 (10 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
作用メカニズム
証明書(CoA、COO、BSE/TSEと分析図)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

4 results found

Lot NumberCertificate Type日付商品
ZJ24F0607491Certificate of AnalysisApr 07, 2026 Ab188096
ZJ24F0607492Certificate of AnalysisApr 07, 2026 Ab188096
ZJ24F0607493Certificate of AnalysisApr 07, 2026 Ab188096
ZJ24R0600677Certificate of AnalysisJun 14, 2024 Ab188096
よくある質問と記事
ソリューション計算機
レビュー

顧客レビュー

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Carrier Free grade guide → View Validated grade guide → View ExactAb™ grade guide → View Azide Free grade guide → View High Performance grade guide → View PBS Only grade guide →

関連商品

Goat Anti-Mouse IgG H&L Antibody - 二次抗体, for ELISA,LF,GICA various applications, from Mouse, generated in Goat
Goat Anti-Mouse IgG H&L (HRP) - 二次抗体, for WB,ELISA,IHC-P various applications, from Mouse, generated in Goat, IgG
Mouse IgG - アイソタイプ対照抗体, generated in Rat, >95%, IgG
Rabbit Anti-Mouse IgG H&L (HRP) - 二次抗体, IgG
BALB/c Mouse IgG - アイソタイプ対照抗体, IgG
Rabbit Anti-Mouse IgG antibody - 二次抗体
Goat Anti-Mouse IgG H&L (FITC) - 二次抗体
Goat Anti-Mouse IgG H&L (TRITC) - 二次抗体
Recombinant SMAD3 Antibody - 一次抗体, specific to SMAD3, Rabbit IgG
Goat Anti-Mouse IgG H&L Antibody - 二次抗体, Goat IgG
Goat Anti-Mouse IgG H&L (Cy5) - 二次抗体
Goat Anti-Mouse IgG H&L (HRP) - 二次抗体
Goat Anti-Mouse IgG H&L (Biotin) - 二次抗体
Rabbit Anti-Mouse IgG (HRP) - 二次抗体
Rabbit Anti-Mouse IgG (Biotin) - 二次抗体
Goat Anti-Mouse IgG H&L (HRP) - 二次抗体, Goat IgG
Goat Anti-Mouse IgG H&L (TRITC) - 二次抗体, Goat IgG
Goat Anti-Mouse IgG H&L (Biotin) - 二次抗体, Goat IgG
Goat Anti-Mouse IgG H&L (FITC) - 二次抗体, Goat IgG
Goat Anti-Mouse IgG H&L (Cy5) - 二次抗体, Goat IgG
Goat Anti-Mouse IgG H&L (PE) - 二次抗体, Goat IgG
Goat Anti-Mouse IgG H&L (APC) - 二次抗体, Goat IgG
Mouse IgG (Biotin) - アイソタイプ対照抗体, Mouse IgG
Mouse IgG (PE) - アイソタイプ対照抗体, Mouse IgG
Mouse IgG (APC) - アイソタイプ対照抗体, Mouse IgG
Mouse IgG (FITC) - アイソタイプ対照抗体, Mouse IgG
Mouse IgG2a-Fc - アイソタイプ対照抗体, Mouse IgG2a
Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) - 二次抗体, Goat IgG
Goat Anti-Mouse IgG H&L (AF405) - 二次抗体, Goat IgG
Goat Anti-Mouse IgG H&L (AF647) - 二次抗体, Goat IgG
Smad3 Mouse mAb - 一次抗体, specific to Smad3, Mouse IgG1
Smad3 Mouse mAb - 一次抗体, specific to Smad3, Mouse IgG2b
Mouse IgG2b-kappa, anti-Spike RBD - アイソタイプ対照抗体, specific to Spike RBD, Mouse IgG2b
Mouse IgG2a-kappa, anti-Spike RBD - アイソタイプ対照抗体, specific to Spike RBD, Mouse IgG2a
Mouse IgG3-kappa, anti-HEL - アイソタイプ対照抗体, specific to HEL, Mouse IgG3
Mouse IgG2c-kappa, anti-HEL - アイソタイプ対照抗体, specific to HEL, Mouse IgG2c
Mouse lgG2A(D265A)-kappa, anti-HEL - アイソタイプ対照抗体, specific to HEL, Mouse IgG2a(D265A)
ヒトIgG1(N297S)-κ、抗HEL - アイソタイプ対照抗体, specific to HEL, Mouse IgG1(D265A)
Mouse IgG (AF488) - アイソタイプ対照抗体, IgG
Mouse IgG (PerCP) - アイソタイプ対照抗体, IgG
Mouse IgG (AF405) - アイソタイプ対照抗体, IgG
Mouse IgG (AF647) - アイソタイプ対照抗体, IgG
Goat Anti-Mouse IgG H&L (AF555) - 二次抗体, specific to Mouse IgG, Goat IgG
Mouse IgG (AF700) - アイソタイプ対照抗体, IgG
Goat Anti-Mouse IgG H&L (AF700) - 二次抗体, specific to Mouse IgG, Goat IgG
マウスIgG (PE-Cy5) - アイソタイプ対照抗体
マウスIgG (PE-Cy7) - アイソタイプ対照抗体
Mouse IgG2b-kappa, anti-E8L - アイソタイプ対照抗体, specific to E8L
Mouse IgG2a-kappa, anti-A29L - アイソタイプ対照抗体, specific to A29L
Mouse IgG2c-kappa (AF647), anti-HEL - アイソタイプ対照抗体, specific to HEL
Mouse IgG2c-kappa (AF700), anti-HEL - アイソタイプ対照抗体, specific to HEL
Mouse IgG2c-kappa (AF405), anti-HEL - アイソタイプ対照抗体, specific to HEL
Mouse IgG2c-kappa (Biotin), anti-HEL - アイソタイプ対照抗体, specific to HEL
Mouse IgG2a-kappa (APC), anti-Spike RBD - アイソタイプ対照抗体, specific to Spike RBD
Mouse IgG2a-kappa (FITC), anti-Spike RBD - アイソタイプ対照抗体, specific to Spike RBD
Mouse IgG2a-kappa (AF700), anti-Spike RBD - アイソタイプ対照抗体, specific to Spike RBD
Mouse IgG2a-kappa (AF647), anti-Spike RBD - アイソタイプ対照抗体, specific to Spike RBD
Mouse IgG2a-kappa (AF555), anti-Spike RBD - アイソタイプ対照抗体, specific to Spike RBD
Mouse IgG2a-kappa (AF405), anti-Spike RBD - アイソタイプ対照抗体, specific to Spike RBD
Mouse IgG2c-kappa (FITC), anti-HEL - アイソタイプ対照抗体, specific to HEL
Mouse IgG2c-kappa (AF555), anti-HEL - アイソタイプ対照抗体, specific to HEL
Mouse IgG2c-kappa (APC), anti-HEL - アイソタイプ対照抗体, specific to HEL
Mouse IgG2c-kappa (PE), anti-HEL - アイソタイプ対照抗体, specific to HEL
Mouse IgG2a-kappa (Biotin), anti-Spike RBD - アイソタイプ対照抗体, specific to Spike RBD
Mouse IgG2a-Fc (Biotin) - アイソタイプ対照抗体
Mouse IgG2a-Fc (FITC) - アイソタイプ対照抗体
Mouse IgG (AF555) - アイソタイプ対照抗体
Mouse IgG2b-kappa (Biotin), anti-Spike RBD - アイソタイプ対照抗体, specific to Spike RBD
Mouse IgG2b-kappa (FITC), anti-Spike RBD - アイソタイプ対照抗体, specific to Spike RBD
Mouse IgG2b-kappa (APC), anti-Spike RBD - アイソタイプ対照抗体, specific to Spike RBD
Mouse IgG2b-kappa (APC-Cy7), anti-Spike RBD - アイソタイプ対照抗体, specific to Spike RBD
Goat Anti-Mouse IgG H&L (PE-Cy5) - 二次抗体, specific to Mouse IgG
Centrose patent anti-dysadherin (anti-Dysadherin) - 一次抗体, specific to FXYD5
Mouse IgG2a-kappa (AF405), anti-A29L - アイソタイプ対照抗体, specific to A29L
Mouse IgG2a-kappa (AF488), anti-A29L - アイソタイプ対照抗体, specific to A29L
Mouse IgG2a-kappa (AF647), anti-A29L - アイソタイプ対照抗体, specific to A29L
Mouse IgG2a-kappa (AF700), anti-A29L - アイソタイプ対照抗体, specific to A29L
Mouse IgG2a-kappa (Biotin), anti-A29L - アイソタイプ対照抗体, specific to A29L
Mouse IgG2a-kappa (FITC), anti-A29L - アイソタイプ対照抗体, specific to A29L
Mouse IgG3-kappa (Biotin), anti-HEL - アイソタイプ対照抗体, specific to HEL
Mouse IgG3-kappa (FITC), anti-HEL - アイソタイプ対照抗体, specific to HEL
Mouse IgG3-kappa (PE), anti-HEL - アイソタイプ対照抗体, specific to HEL
Mouse IgG3-kappa (AF488), anti-HEL - アイソタイプ対照抗体, specific to HEL
Mouse IgG3-kappa (APC), anti-HEL - アイソタイプ対照抗体, specific to HEL
Mouse IgG3-kappa (AF647), anti-HEL - アイソタイプ対照抗体, specific to HEL
Mouse IgG2b-kappa (AF555), anti-Spike RBD - アイソタイプ対照抗体, specific to Spike RBD
Goat Anti-Mouse IgG H&L (AF488) - 二次抗体
Goat Anti-Mouse IgG H&L (PE-Cy7) - 二次抗体
Mouse IgG (PE-Cy5.5) - アイソタイプ対照抗体
Goat Anti-Mouse IgG H&L (AF750) - 二次抗体
Mouse IgG2a-kappa (AF555), anti-A29L - アイソタイプ対照抗体, specific to A29L
Mouse IgG2a-kappa (PE-Cy5.5), anti-A29L - アイソタイプ対照抗体, specific to A29L
Goat Anti-Mouse IgG H&L (AF546) - 二次抗体
Goat Anti-Mouse IgG H&L (Oregon Green 488) - 二次抗体
Goat Anti-Mouse IgG H&L (Cy3) - 二次抗体
Mouse IgG2a-kappa (APC), anti-A29L - アイソタイプ対照抗体, specific to A29L
Mouse IgG3-kappa (AF555), anti-HEL - アイソタイプ対照抗体, specific to HEL
Mouse IgG (PE-AF647) - アイソタイプ対照抗体

Shall we send you a message when we have discounts available?

Remind me later

Thank you! Please check your email inbox to confirm.

Oops! Notifications are disabled.