Stabilizers for HRP conjugates - STplus

Cat. No.: S491386

GRADE & PURITY STplus

Storage

Store at 2-8°C

Shipped In

Wet ice

Overview

1、Product attribute

Shelf life: 36 months

pH Range: 7.1-7.7

Preservative: CMIT/MIT

Boiling point : 100℃

Appearance: colourless - pale yellow liquid

Density (20℃) : 1.043 g/cm³   

Water solubility: easily soluble

Odour: odourless

Heat sensitive

2、Requirements for storage rooms and vessels

1.Keep container tightly closed.

2.Keep cool.

3.Do not store together with: Oxidizing agent.

4. It is possible to transport the solution at ambient temperature, unless it does not exceed 30 °C. The transport should take less than one week.

3、Effective Components and Principle of Function       

It is based on a TRIS buffer and especially suitable for conjugates of sensitive monoclonal antibodies

For stabilization of HRP conjugates the solutions provide optimum ambient conditions regarding pH value, ionic strength, hydrophilic-hydrophobic as well as ionic interactions, needed to safe the conformation and function of the enzyme as well as the antibody or biomolecule.

4、Biosafety information

The solutions contain biocides for protection from microbiological spoilage.

This mixture is not classified as hazardous in accordance with Regulation (EC) No 1272/2008；

It contains reaction mass of 5-chloro-2-methyl-2H-isothiazol-3-one and 2-methyl-2H-isothiazol-3-one (3:1). May produce an allergic reaction.

5、Advantage

1. Stabilizer for conjugates with monomeric HRP

2. High signal-to-noise ratio

3. Effective preservation of enzyme activity

6、Instruction for usage

1. Any handling to fill in other vessels or splitting has to be done under sterile conditions.

2. Frozen solutions have to be mixed thoroughly after thawing and can be used without restriction afterwards.

3.Solutions showing turbidity should not be used. Since this might be a sign of microbial contamination.

4. Only qualified staff, who are familiar with the production of immunological tests, is permitted to handle this product.

5. The solutions contain bovine serum albumin. This may result in slight shifts of the product properties. Therefore it is recommend to check every new lot for its application in the planned test systems. A reservation of distinct lots is possible.

6. The stabilizer solution is applied undiluted. The biomolecules should be diluted stepwise in the stabilizer solution, depending on the initial concentration and the planned final concentration. Depending on the requirements of the test system concentrations between 1ng/ml and more than 5 µg/ml are suitable.

7. Conjugate solutions on the basis of this product is suitable for ELISA, micro arrays and membrane based assays.

8. To suppress undesirable effects it is possible to add e.g. HAMA binder and / or further species specific proteins to the solutions.

9. For identification purposes it is possible to add dyes. In case of a request, please contact us.

7、.Special details / Limitations
1.Under the influence of elevated temperatures the solution develops a darker colour. The stabilizing effect is not influenced.

Specifications

Specifications & Purity

STplus

Storage

Store at 2-8°C

Shipped In

Wet ice

This product requires cold chain shipping. Ground and other economy services are not available.

Chemical and Physical Properties

• Sensitivity: heat sensitive