GRADE & PURITYBioactive?Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure.Recombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.ActiBioPure™?ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.EnzymoPure™?EnzymoPure™ — Aladdin's line of high-quality enzymatic solutions. Use when enzyme purity and defined activity drive assay or process performance.≥95%(SDS-PAGE)1 mg/ml
Accession #
P00720
Expression system
E. coli
Bioactivity
A 200 μl reaction in 50 mM Tris-HCl pH 8.0 buffer containing a Micrococcus lysodeikticus suspension and 1 μg of T4 Lysozyme (E. coli) incubated for 20 minutes at 25°C results in a decrease of ≥0.05 in turbidity as determined by OD₄₅₀.
Bioactive,Recombinant,ActiBioPure™,High Performance,EnzymoPure™,≥95%(SDS-PAGE),1 mg/ml ActiBioPure™,Bioactive,High Performance,Recombinant,EnzymoPure™ for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
概要
Bacteriophage T4 Lysozyme breaks down bacterial cell walls. The enzyme attacks the peptidoglycans in the cell walls of bacteria and hydrolyzes the ß-1,4 linkages between N-acetylmuramic acid and N-acetylglucosamine. Specific activity of T4 lysozyme is significantly greater than egg white lysozyme when assayed with Micrococcus lysodeikticus and E. coli.
Protein Purity: ≥95% (SDS-PAGE)
Storage Buffer: 20 mM Tris-HCl, 250 mM NaCl, 1 mM DTT, 0.1mM EDTA, 50% Glycerol, pH 8.0.
Activity: 200 μl reaction in 50 mM Tris-HCl pH 8.0 buffer containing a Micrococcus lysodeikticussuspension and 1μg of T4 Lysozyme (E. coli) incubated for 20 minutes at 25°C results in a decrease of ≥0.05 in turbidity as determined by OD₄₅₀.
Applications:
Bacterial lysis for nucleic acid extraction
Bacterial lysis for recombinant protein extraction
Source:
A recombinant E. coli strain carrying the cloned bacteriophage T4 lysozyme gene.
Specifications
Product Name
T4 Lysozyme
同義語
muramidase | Endolysin
等級
ActiBioPure™, Bioactive, High Performance, Recombinant, EnzymoPure™
Endolysin with lysozyme activity that degrades host peptidoglycans and participates with the holin and spanin proteins in the sequential events which lead to the programmed host cell lysis releasing the mature viral particles. Once the holin has permeabil
生物活性
A 200 μl reaction in 50 mM Tris-HCl pH 8.0 buffer containing a Micrococcus lysodeikticus suspension and 1 μg of T4 Lysozyme (E. coli) incubated for 20 minutes at 25°C results in a decrease of ≥0.05 in turbidity as determined by OD₄₅₀.
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よくある質問
What is the purity of this product, and how is it determined?
This product is supplied at ≥95% purity, determined by SDS-PAGE. Lot-specific values are stated on the Certificate of Analysis.
What does Bioactive mean?
Bioactive indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.
What does Recombinant mean?
Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.
How should this product be stored?
Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.
How is this product shipped?
This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.
What documentation is provided?
Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.
Need help choosing the grade?
Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.
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