T6167923 - ≥99% , CAS No.2437475-16-4

CAS: 2437475-16-4 Cat. No.: T646230 分子式: C₁₇H₂₀BrN₃O₃S₂ 分子量: 458.39

GRADE & PURITY ≥99%

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

概要

T6167923 is a selective inhibitor of MyD88-dependent signaling pathways. T6167923 directly binds to Toll/IL1 receptor (TIR) domain of MyD88 and disrupts MyD88 homodimeric formation. T6167923 inhibits NF-κB driven Staphylococcus enterotoxin AP (SEAP) activity, and improves anti-inflammatory activity with IC 50 s of 2.7  μM, 2.9 μM, 2.66 μM and 2.66 μM for IFN-γ , IL-1β, IL-6 and TNF-α, respectively

In Vitro

T6167923 (0-500 μM; 20 h) inhibits the pro-inflammatory cytokine response of staphylococcal enterotoxin B (SEB) in peripheral blood mono nuclear cells. T6167923 (10-500 μM; 2 h) inhibits secreted alkaline phosphatase response (SEAP) expression in HEK 293T cells. T6167923 (100 μM; 16 h) binds to TIR protein and reduced the inhibitory effect on MyD88-signaling. T6167923 (1-500 μM; 13 h) inhibits full-length MyD88 homodimeric formation. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Cell Viability AssayCell Line: Peripheral blood mono nuclear cells Concentration: 0-500 μM Incubation Time: 20 hours Result: Dose-dependently attenuated the response of SEB to TNF-α, INF-γ, IL-6, and IL-1β with IC 50 s of 2.66, 2.7, 2.66 and 2.9 μM in peripheral blood mono nuclear cells. Cell Viability AssayCell Line: HEK 293T cell line Concentration: 10-500 μM Incubation Time: 2 hours Result: Dose-dependently inhibited lipo-polysaccharide (LPS) induced MyD88-mediated NF-kB driven SEAP expression in HEK 293T cells with IC 50 s in the range of 40–50 μM. Cell Viability AssayCell Line: HEK 293T cell line Concentration: 100 μM Incubation Time: 16 hours Result: Specifically targeted MyD88 and dose-denpendently with TIR protein to reduced the inhibitory effect of MyD88-signaling. Western Blot AnalysisCell Line: HEK 293-I3A cells with MyD88 knockout Concentration: 1-500 μM Incubation Time: 13 hours Result: Dose-dependently inhibited TIR domain-mediated dimerization of full-length MyD88 and the recombinant TIR domain protein.

In Vivo

T6167923 (0.17 and 1 mg; i.p. once) survives the mice from intoxication with SEB and LPS injection. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Animal Model: 16-20 week-old BALB/c mice with LPS potentiation modelDosage: 0.17 and 1 mg Administration: Intraperitoneal injection; 0.17 and 1 mg once Result: Dose-dependently showed a therapeutic efficacy against SEB intoxication.

Form:Solid

IC50& Target:IC50: 2.7 μM (IFN-γ), 2.9 μM (IL-1β), 2.66 μM (IL-6), 2.66 μM (TNF-α)

Specifications

仕様と純度

≥99%

生化学的・生理学的メカニズム

T6167923 is a selective inhibitor of MyD88-dependent signaling pathways. T6167923 directly binds to Toll/IL1 receptor (TIR) domain of MyD88 and disrupts MyD88 homodimeric formation. T6167923 inhibits NF-κB driven Staphylococcus enterotoxin AP (SEAP) activ

保管条件

Store at -20°C

入荷

Ice chest + Ice pads

この製品は冷冷この冷このこの製品の運送が必要です。地上およびその他の経済サービスは利用できません。

純度

≥99%

名前と識別子

カノニカル・スマイル

BrC1=CC(S(=O)(N2CCN(C(N[C@H](C3=CC=CS3)C)=O)CC2)=O)=CC=C1

分子量

458.39

化学的性質と物理的性質

• 溶解度: DMSO : 250 mg/mL (545.39 mM; Need ultrasonic) H2O : <0.1 mg/mL (ultrasonic;warming;heat to 60°C) (insoluble)

よくある質問

What is the purity of this product?

This product is supplied at ≥99% purity (chemical assay). Lot-specific values are stated on the Certificate of Analysis.

How should this product be stored?

Store at ?20 °C. Freezer storage is required to maintain the specified shelf life.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 2437475-16-4, the molecular formula is C17H20BrN3O3S2, and the molecular weight is 458.39 g/mol.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.