Thienopyridone - 10mM in DMSO , CAS No.1018454-97-1

CAS: 1018454-97-1 Cat. No.: T655844 分子式: C₁₃H₁₀N₂OS 分子量: 242.30 EC番号: 819-423-4 PubChem CID: 91383855

GRADE & PURITY 10mM in DMSO

Storage

Store at -80°C

Shipped In

Dry ice packs + Cold packs

概要

Thienopyridone is a potent and selective phosphatase of regenerating liver (PRL) phosphatase inhibitor with IC 50 s of 173 nM, 277 nM and 128 nM for PRL-1 , PRL-2 , and PRL-3 , respectively. Thienopyridone shows minimal effects on other phosphatases. Thienopyridone induces p130Cas cleavage and apoptosis and has anticancer effects

In Vitro

Thienopyridone shows significant inhibition of tumor cell anchorage-independent growth in soft agar. The EC 50 values of the Thienopyridone are 3.29 μM and 3.05 μM for RKO and HT-29 cells, respectively. Thienopyridone (1-75 μM; 24 hours; HeLa cells) treatment shows a dose-dependent down-regulation of total p130Cas in HeLa cells. Thienopyridone induces p130Cas and FAK cleavage leads to caspase-mediated cell apoptosis. Thienopyridone induces the cleavage of PARP and caspase-8. Thienopyridone (3.75-30 μM; 24 hours) significantly suppresses HUVEC migration but not proliferation. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Cell Viability AssayCell Line: RKO and HT-29 cells Concentration: 0.5 μM, 1.67 μM, 5 μM, 8.33 μM Incubation Time: 14 days Result: Exhibited a dose-dependent inhibition in cancer cell anchorage-independent growth as measured by either colony number or colony size. Western Blot AnalysisCell Line: HeLa cells Concentration: 1 μM, 5 μM, 10 μM, 25 μM, 50 μM, 75 μM Incubation Time: 24 hours Result: A dose-dependent down-regulation of total p130Cas was observed.

IC50& Target:IC50: 173 nM (PRL-1), 277 nM (PRL-2) and 128 nM (PRL-3)

Specifications

仕様と純度

10mM in DMSO

生化学的・生理学的メカニズム

Thienopyridone is a potent and selective phosphatase of regenerating liver (PRL) phosphatase inhibitor with IC 50 s of 173 nM, 277 nM and 128 nM for PRL-1 , PRL-2 , and PRL-3 , respectively. Thienopyridone shows minimal effects on other phosphatases. Thie

保管条件

Store at -80°C

入荷

Dry ice packs + Cold packs

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名前と識別子

異性体SMILES

C1=CC=C(C=C1)C2=CC3=C(S2)C(=CNC3=O)N

PubChem CID

91383855

分子量

242.30

よくある質問

How should this product be stored?

Store at ?80 °C. Ultra-low temperature storage is required to maintain the specified shelf life.

How is this product shipped?

This product ships frozen with dry ice and cold packs. Unpack on arrival and transfer it to the storage condition stated above; handle dry ice with insulated gloves in a ventilated area.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 1018454-97-1, the molecular formula is C13H10N2OS, and the molecular weight is 242.30 g/mol.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.