Tissue Storage Solution is a ready‑to‑use low‑temperature transport and storage solution for short‑term storage and transport of fresh human or animal tissue samples. It effectively prevents apoptosis, necrosis, and other damage during storage or transport, thereby maintaining cell functionality and viability. This product preserves excised tissues at 2–8 °C for up to 72 hours and is compatible with the following applications:
Primary cell isolation and culture
Preparation of single‑cell suspensions (for flow cytometry, single‑cell sequencing, etc.)
RNA extraction and sequencing (ensures RNA integrity, RIN ≥ 8)
Histopathological sectioning
Storage Instructions
Unopened solution: Store at –20 °C, protected from light. Shelf life: 12 months.
Opened solution: Store sealed at 4 °C; recommended for use within 7 days.
Tissue Preservation Conditions: Excised tissue must be completely submerged in the preservation solution and stored at 4 °C for a maximum of 72 hours.
Tissue Handling
Immediately after excision, immerse the tissue in 75% ethanol for 5 minutes, rinse with sterile PBS, and then place it into the preservation solution as soon as possible (volume of solution ≥ 5× tissue volume). Avoid drying. For large tissues, cut into pieces no thicker than 5 mm to ensure adequate penetration of the preservation solution.
Preservation and Transport
Store statically at 4 °C, avoiding vigorous shaking or repeated freeze‑thaw cycles. For long‑distance transport, use ice packs or a refrigerated box (maintain 4 °C).
Subsequent Experimental Procedures
Primary cell isolation: After removing the tissue, rinse twice with pre‑chilled sterile PBS, then proceed with standard digestion protocols.
Single‑cell isolation: Remove residual preservation solution before mechanical or enzymatic dissociation.
RNA extraction: Transfer the tissue directly into lysis buffer, or snap‑freeze in liquid nitrogen and store at –80 °C for long‑term preservation.
Precautions
Do not freeze the tissue in the preservation solution, as ice crystals will damage cell structure.
Storage for more than 72 hours may reduce cell viability or cause RNA degradation. Process the tissue as soon as possible.
When used for RNA sequencing, always wear gloves and use RNase‑free consumables throughout the procedure.
For long‑term tissue preservation (>72 hours), it is recommended to replace with fresh preservation solution or aliquot and freeze (compatibility must be verified).
This product is for research use by qualified professionals only. Not intended for clinical diagnosis, treatment, or food/drug applications.
For your safety and health, please wear a lab coat and disposable gloves during handling.
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