グレード＆純度Moligand™?Moligand™ — Aladdin's line of ligands and bioactive small molecules. Use for receptor, pathway, and binding studies needing defined small-molecule tools.
This compound belongs to the class of organic compounds known as gallic acid and derivatives. These are compounds containing a 3,4,5-trihydroxybenzoic acid moiety.
External Descriptors
Not available
1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.
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Application Protocols
No item-specific, validated protocols are provided in the product data. The following general protocol outlines typical usage of small-molecule probes in cell-based Ca2+ imaging assays:
General protocol (example):
Stock solution: Dissolve compound in anhydrous DMSO at 10–50 mM. Aliquot and store desiccated.
Cell preparation: Plate cells on glass-bottom dishes or black, clear-bottom plates and culture to desired confluence.
Indicator loading: Incubate with a Ca2+-sensitive dye (e.g., Fluo-4 AM) under standard loading conditions; wash and equilibrate in assay buffer (e.g., HEPES-buffered HBSS).
Dosing: Prepare serial dilutions of the compound in assay buffer containing the same final DMSO as vehicle control (e.g., 0.1%). Add compound and record fluorescence over time.
Controls: Include vehicle-only, positive control for Ca2+ mobilization, and, if possible, an orthogonal inhibitor to assess specificity.
Data analysis: Normalize to baseline, quantify peak ΔF/F0 and area under the curve, and fit concentration–response curves to estimate potency.
Adapt volumes, timings, and readouts to your instrument and cell model. Validate that the vehicle and compound do not interfere with the detection modality.
Biological Roles
Research context (literature, non-item-specific):
TMB-8 has been widely used as a tool compound to interrogate intracellular calcium (Ca2+) signaling. Reports describe it as an antagonist of Ca2+ mobilization from intracellular stores, enabling dissection of pathways involving store release and downstream Ca2+-dependent processes.
By modulating Ca2+ fluxes, TMB-8 is used to probe roles of Ca2+ in secretion, contraction, gene expression, and other signaling cascades in various cell types and model systems.
Key considerations for biological studies:
Specificity: Literature indicates that effects can be context- and concentration-dependent, with potential off-target or pleiotropic actions at higher concentrations. Always include orthogonal controls and, if possible, alternate chemotypes to confirm mechanistic conclusions.
Kinetics and reversibility: Onset and reversibility of action may vary with cell type, temperature, and extracellular Ca2+; time-course experiments are recommended.
Compatibility with readouts: Verify that the compound and vehicle do not interfere with fluorescence indicators, bioluminescent assays, or reporter systems.
Important disclaimer:
The above summarizes general research use cases. This product is provided strictly for research use only and is not intended for diagnostic, therapeutic, or other clinical applications.
Buffer Applications
This product is not a buffering reagent and is not used to set or maintain solution pH.
Practical notes for using TMB-8 in buffered systems (general):
Vehicle control: When dosing from DMSO or ethanol stocks into buffers or media, include a matched vehicle control to isolate compound effects from solvent effects.
Order of addition: Add the compound last to pre-equilibrated buffer/media with constant mixing to reduce precipitation risk. Avoid concentrated additions to small volumes.
Protein binding: Serum proteins and albumin can alter free concentration; if working in protein-containing buffers, consider binding effects and adjust dosing accordingly.
Adsorption: Hydrophobic ligands may adsorb to plastic; precondition wells or use low-binding plastics/glassware as needed.
For buffer composition, pH range, and ionic strength, use the buffer system required by your biological assay (e.g., HEPES, PBS, HBSS) and confirm compound stability therein.
Green Alternatives
As a bioactive small-molecule probe, TMB-8 is not a process solvent or bulk reagent where typical “green replacement” strategies directly apply. Nevertheless, greener handling of probe solutions is feasible.
Greener practice suggestions (general):
Minimize DMSO: Use the lowest effective DMSO percentage in assays (≤0.1–0.2% v/v when compatible) to reduce solvent burden and vehicle effects.
Ethanol as an alternative vehicle: Where compatible with your biology, ethanol can substitute part of DMSO; beware of volatility and evaporation-driven concentration changes.
Aqueous-first approaches: If solubility allows, use aqueous buffers with cyclodextrins or mild acid/base adjustments to limit organic solvents.
Waste reduction: Consolidate solvent waste streams and use microvolume assay formats (384/1536-well) to decrease total solvent usage.
Balanced trade-offs:
DMSO offers superior solubilizing power and chemical stability but increases solvent exposure in biology; ethanol is greener but may limit solubility and introduces volatility.
Cosolubilizers (e.g., captisol, cyclodextrins) can reduce organic content but may impact assay readouts; validate carefully.
Select the approach that preserves data quality while reducing environmental and health footprints.
Pharmaceutical Uses
This product is supplied for research use only. It is not manufactured to GMP standards and is not intended for human or veterinary use, diagnostic procedures, or as an excipient.
Research/manufacturing context (general):
In preclinical research, small-molecule probes like TMB-8 may be used to study pathways relevant to discovery programs. Any use beyond nonclinical laboratory studies would require dedicated quality, regulatory, and safety evaluations not covered by this product.
No pharmacopeial monograph or compendial status is provided for this item. Excipient functionality, residual solvent limits, elemental impurity limits, and bioburden/endotoxin controls are not specified.
For any application requiring GMP-grade materials, validated impurity profiles, or full regulatory documentation, please request an alternative grade or a custom manufacture with appropriate quality agreements.
Physical Properties
Item-specific physico-chemical specifications are not provided in the product data. Please consult the CoA/Spec Sheet for definitive values.
Appearance: Not specified for this item; refer to CoA/Spec Sheet.
Melting/boiling point: Not specified for this item; refer to CoA/Spec Sheet.
Density, refractive index: Not specified for this item; refer to CoA/Spec Sheet.
pKa, logP: Not specified for this item; refer to CoA/Spec Sheet.
Solubility: Not specified for this item; refer to CoA/Spec Sheet.
General handling considerations (literature/general guidance):
Many bioactive screening ligands of this class are lipophilic weak bases that dissolve readily in anhydrous DMSO, with moderate solubility in ethanol or methanol; aqueous solubility may be limited without cosolvent or acidification.
For cell assays, prepare DMSO stocks (e.g., 10–50 mM), then dilute into buffer/media to a final DMSO content typically ≤0.1–0.5% v/v, verifying absence of precipitation.
Always treat literature-based expectations as starting points only. Establish solubility and stability for your specific lot by small-scale trials and analytical checks (e.g., visual inspection, HPLC/UPLC).
Quality and Grades
Grade/Purity: Moligand™ (as provided). Moligand is intended for discovery screening, target validation, and chemical biology workflows—not for diagnostic or therapeutic use.
What Moligand™ typically implies (general guidance):
Emphasis on suitability for biological screening and SAR exploration, with routine identity confirmation and purity appropriate for research assays. Unless otherwise stated on the CoA, it is not a GMP, pharmacopeial, or clinical grade.
UV/LC characterization, mass, and/or NMR data are typically used to establish lot identity and purity; consult the lot-specific CoA for the exact test suite and acceptance criteria.
Item-specific notes:
Purity percentage, stabilizers, residual solvent limits, water content, metal content, and UV cutoffs: Not specified for this item; refer to CoA/Spec Sheet.
If your application requires additional analytical documentation (orthogonal identity tests, residuals, elemental analysis), contact Technical Support with the SKU and lot number to request available data or a custom qualification plan.
Reaction and Applications
This product is primarily intended as a research probe/screening ligand rather than a synthetic reagent.
TMB-8 has been widely reported in the literature as a chemical tool for modulating intracellular Ca2+ dynamics, particularly to study Ca2+ release from intracellular stores and downstream signaling. It is often used in cell-based assays, fluorescence calcium imaging, or pathway interrogation experiments.
Practical usage notes for bioassays:
Prepare concentrated DMSO stocks (e.g., 10–50 mM), filter if needed, and store aliquots to minimize freeze–thaw.
Determine working concentration empirically via pilot dose–response (e.g., 0.1–100 µM), including a vehicle control and appropriate positive/negative controls.
Verify compound integrity during the assay window (co-inject LC at T0/Tend, or use a stability-indicating method) if exposures exceed several hours or if media contain reactive components (e.g., high serum, strong oxidants).
For Ca2+ imaging, synchronize dosing with indicator loading/washout to avoid vehicle effects on fluorescence; monitor cell health.
Note: The above are general best practices for small-molecule probes. For your specific lot of TMB-8, rely on the CoA/SDS and your internal qualification data to finalize working conditions.
Reaction Conditions
Not applicable as a classical synthetic reagent. However, for biological assay deployment, the following general handling conditions are commonly employed for small-molecule probes:
Stock preparation: Dissolve in anhydrous DMSO to 10–50 mM; vortex and sonicate briefly if needed. Filter through 0.22 µm PTFE if particulate persists.
Working solutions: Dilute into prewarmed buffer or culture medium with rapid mixing to final DMSO ≤0.1–0.5% v/v. Verify clarity and absence of precipitation.
Temperature/exposure: Typical cell-based exposures range from minutes to several hours at 25–37°C, depending on assay design. Confirm stability over the exposure period.
Light/oxidation: Protect from strong light and oxidants if the compound or vehicle is light/oxygen sensitive; use amber vials and inert headspace for long incubations when warranted.
Controls: Include vehicle controls and, where appropriate, positive controls targeting the same pathway to contextualize results.
These are general guidelines; optimize conditions empirically for your model system and confirm with the lot-specific CoA/SDS.
Safety and Handling
GHS and hazard details are not listed in the product data. Refer to the SDS for authoritative information.
Signal word / H-statements / GHS classification / pictograms: Not specified for this item; refer to SDS.
Incompatibilities (general): Strong oxidizers and strong acids/bases may cause degradation of organic small molecules; avoid moisture if the compound is hygroscopic.
First-aid overview: If on skin/eyes, rinse with water for at least 15 minutes; if inhaled, move to fresh air; if ingested, seek medical attention. Follow institutional protocols and SDS.
Spill response: Absorb with inert material, collect for disposal, and ventilate area. Prevent release to drains.
Special risks (general for screening ligands):
Potential for dust generation with solids; minimize dust and static ignition sources.
Solutions in organic solvents (e.g., DMSO, ethanol) may increase dermal penetration risk—avoid skin exposure.
Disposal: Dispose of contents/container in accordance with local/regional regulations and your EHS guidance.
Solvent Selection
This product is a bioactive small molecule rather than a process solvent. Solvent choice here concerns dissolving the compound for assay use.
General guidance for small-molecule probe preparation:
Primary solvent: Anhydrous DMSO is commonly preferred for initial stock solutions due to its strong solubilizing power for lipophilic ligands and good compatibility at low final percentages in biological media.
Secondary options: Ethanol or methanol may also dissolve many ligands; verify assay compatibility and cytotoxicity of the vehicle.
Aqueous use: Dilute DMSO stocks into buffer or media with vigorous mixing to avoid localized supersaturation. Keep final DMSO ≤0.1–0.5% v/v where possible.
pH adjustments: For weak bases, limited aqueous solubility can sometimes be improved with slight acidification (e.g., addition of dilute HCl) after confirming compound stability.
Comparison (general):
DMSO: highest solvating power, low volatility; potential to affect membrane permeability—control for vehicle.
Ethanol: more volatile, acceptable at ≤0.1–0.5% in many assays; watch for evaporation-induced concentration drifts.
Shipped in: Not specified for this item; refer to CoA/Spec Sheet.
General best practices:
Solid material: Store tightly capped in a desiccator at room temperature, protected from light. Allow container to equilibrate to room temperature before opening to minimize moisture uptake.
Reconstitution: Prepare concentrated stocks in anhydrous DMSO. Record exact mass and solvent volume to calculate stock concentration accurately.
Aliquoting: Dispense single-use aliquots to avoid repeated freeze–thaw and headspace moisture exposure.
Solution storage (general): DMSO stocks are commonly stored at −20°C or below, protected from light and moisture. Warm to room temperature and mix thoroughly before use; inspect for precipitation or discoloration.
Stability: Solution and solid-state stability are not specified for this item; refer to CoA/Spec Sheet and verify with your internal QC (e.g., LC-MS) at defined intervals.
Always follow your institution’s chemical management and documentation practices, and consult the SDS for detailed handling and storage guidance.
Structure and Identity
TMB-8 is supplied as a research-use small molecule (Moligand grade) commonly used as a bioactive ligand in screening campaigns.
SKU: T614493
Product name: TMB-8
CAS: 57818-92-5 (as provided for this item)
Category: Small molecules and compound libraries (可售 / 小分子和化合物库)
Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
SMILES: Not specified for this item; refer to CoA/Spec Sheet.
InChIKey: Not specified for this item; refer to CoA/Spec Sheet.
Structural features (general literature context, not item-specific):
Literature sources describe “TMB-8” as a lipophilic, tertiary amine–containing small molecule used as a calcium-mobilization antagonist in cell biology. Exact stereochemistry and substitution pattern for this catalog lot are not specified here; consult the item’s CoA for definitive identifiers.
Note: When structural identifiers are critical for your work (e.g., cheminformatics registration, analytical qualification), please verify using the lot-specific CoA/SDS before use.
Synthetic Utility
TMB-8 is supplied as a bioactive probe and is not primarily used as a synthetic building block or reagent.
General remarks for chemists considering derivatization (literature-level, non-item-specific):
If the literature structure is used as a starting point, modifications typically focus on tuning basicity/lipophilicity or altering aromatic/amine substituents to explore SAR around calcium-mobilization activity.
Late-stage diversification strategies may include amide/urethane formation on available amines (if present), or ester exchange on putative ester functions, subject to the actual structure confirmed on the CoA.
Stability awareness: Basic centers and ester linkages (if present) can be susceptible to hydrolysis or transesterification under harsh conditions; protect or adjust conditions accordingly.
Because structural identifiers for this catalog entry are not specified in the provided data, any synthetic planning should begin with analytical confirmation of the exact structure and functional groups of the lot in hand.
Target Specificity
This product is not an antibody or a biologic; antigen/epitope/isotype details do not apply.
Literature perspective (non-item-specific):
TMB-8 has been described as a chemical modulator of intracellular Ca2+ mobilization with broad, context-dependent effects. A single, definitive molecular target is not universally established across systems, and activity can vary by cell type and concentration.
For target-validation studies, employ orthogonal chemical probes and genetic approaches (e.g., RNAi/CRISPR) to assess specificity, and confirm phenotype with multiple tool compounds.
よくある質問
How should this product be stored?
Store at room temperature.
What is Moligand??
Moligand? is one of Aladdin Scientific's premium product lines, formulated for high-purity applications across multiple workflows. Compared to standard grades in the same workflow, Moligand? products typically offer tighter specifications, more rigorous QC, and stronger lot-to-lot consistency.
What are the CAS number, molecular formula and molecular weight?
The CAS Number is 57818-92-5. InChIKey IBQMHBGFMLHHLE-UHFFFAOYSA-N.
What documentation is provided?
Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.
Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
