GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.1.0 mg/mL
TRIM5 alpha Mouse mAb (Ab132527) - Western Blot All lanes: TRIM5 alpha Mouse mAb (Ab132527) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 56 kDa Observed band size: 57 kDa Exposure time: 67.2 s
TRIM5 alpha Mouse mAb (Ab132527) - IP Immunoprecipitation was performed by incubation of 2.5 µg of TRIM5 alpha Mouse mAb (Ab132527) with Jurkat lysate containing 200 µg of total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. TRIM5 alpha Mouse mAb (Ab132527) at 1 µg/mL was used as the primary antibody and peroxidase conjugated rabbit anti-mouse IgG (Light chain specific) was used as the secondary antibody. Lane 1: Jurkat lysate Lane 2: TRIM5 immunoprecipitated from Jurkat lysate by TRIM5 alpha Mouse mAb (Ab132527) Lane 3: The isotype control antibody instead of TRIM5 alpha Mouse mAb (Ab132527) in HeLa lysate. Result: TRIM5 alpha Mouse mAb (Ab132527) can immunoprecipitate TRIM5.
適用＃テキヨウ＃
適用＃テキヨウ＃
Dilution info
WB
1/1000
IP
1/100
Documentation
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よくある質問
What does ExactAb? mean?
ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.
What does Carrier Free mean?
Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.
How should this product be stored?
Store at ?80 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.
How is this product shipped?
This product ships frozen with dry ice and cold packs. Unpack on arrival and transfer it to the storage condition stated above; handle dry ice with insulated gloves in a ventilated area.
What documentation is provided?
Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.
Which species reactivity has this antibody been validated against?
Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (TRIM5 alpha Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.
Need help choosing the grade?
Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.
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