グレード＆純度Moligand™?Moligand™ — Aladdin's line of ligands and bioactive small molecules. Use for receptor, pathway, and binding studies needing defined small-molecule tools.10 mM in DMSO
UPCDC30766 is a potent allosteric inhibitor of p97 , with an IC 50 of 12 nM. UPCDC30766 can be used for the research of colon cancer. UPCDC30766 is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.
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Application Protocols
No item-specific, validated protocols are provided for UPCDC30766. The following are general, literature-based examples for small-molecule screening compounds; adapt to your system.
Example 1: Preparing DMSO stocks and assay dilutions (general)
Dissolve compound at 10 mM in anhydrous DMSO. Vortex and sonicate briefly if needed.
Aliquot into low-bind tubes (e.g., 10–50 µL) and store at −80°C. Avoid repeated freeze–thaw.
For a 384-well biochemical assay, perform serial 1:3 DMSO dilutions to generate a dose–response (e.g., 10 mM → 0.5 mM). Transfer small volumes to assay buffer to reach 0.1–1% DMSO final.
Example 2: Thermal shift assay (DSF/TSA) setup (general)
Prepare protein in 20–50 mM HEPES or PBS, 150 mM NaCl, pH 7.4, with 2–5× dye.
Add compound to desired final concentration range (e.g., 1–100 µM) at constant DMSO percentage. Run melt curve and analyze ΔTm.
Example 3: Cellular assay dosing (general)
Prepare intermediate dilutions in culture medium with matched DMSO (≤0.1–0.5% v/v as cell-type permits). Monitor for precipitation and cytotoxicity of the vehicle.
Note: These are generic templates, not specifications for this item. Validate conditions empirically.
Biological Roles
Item-specific biological function, target, or mechanism of action for UPCDC30766 is not provided in the product data.
General context for screening compounds (not item-specific claims)
Screening ligands are used to probe biochemical pathways, protein–ligand interactions, or cellular phenotypes. They may act via diverse mechanisms such as competitive binding, allosteric modulation, covalent modification (if electrophiles are present), or pathway-level effects.
Interference risks: Some chemotypes can display assay interference (fluorescence quenching, aggregation, redox cycling). Incorporating counterscreens and orthogonal readouts mitigates false positives.
Important
No biological role, target specificity, or pathway assignment is made for this item. Any biological observations should be generated de novo in your system and independently confirmed. All uses are for research only.
Buffer Applications
This product is not a buffer reagent. However, buffers are integral to its use in assays. No item-specific pKa or ionic properties are available.
General guidance (not item-specific)
Common assay buffers: PBS, HEPES, Tris, or MOPS at 10–50 mM, with 0.1–0.5% v/v DMSO to solubilize small molecules. Include 0.01–0.05% non-ionic surfactant if aggregation is suspected.
pH: Select buffer pH to match the biological target and maintain compound stability; many assays operate between pH 6.5–7.5.
Additives: 0.1–1% BSA can mitigate nonspecific binding for sticky compounds; Mg2+/Mn2+/Ca2+ only if target biology requires.
Preparation notes
Pre-dilute compound in DMSO to a high concentration, then add to buffer as a small aliquot to achieve the final solvent percentage uniformly across all conditions.
Item-specific buffer capacities, pKa values, or recipes: Not specified for this item; refer to your assay SOPs.
Green Alternatives
Given the typical use of UPCDC30766 as a screening compound, the main environmental considerations relate to solvent choice and assay workflow rather than the compound itself. No item-specific green metrics are provided.
Greener handling strategies (general, not item-specific)
Minimize DMSO carryover by using the lowest feasible final co-solvent percentage (often 0.1–0.5% v/v) consistent with solubility.
Prefer aqueous buffers with small co-solvent fractions over neat organic media when assay-compatible.
Consolidate plate maps and employ acoustic dispensing to reduce solvent waste.
Illustrative comparison (general)
DMSO: High solvency, miscible with water; broadly compatible but with moderate EHS burden.
Ethanol: Renewable and lower toxicity profile; limited solvency for more lipophilic chemotypes and potential biological effects at ≥1% v/v.
2‑Propanol: Similar to ethanol; evaporates faster and can cause dosing variability.
Waste management
Collect DMSO- and DMF-containing waste separately for proper disposal. Avoid halogenated solvents unless required by downstream analytics.
Note: No specific alternatives tied to the structure of UPCDC30766 can be recommended without disclosed functional groups or stability data.
Pharmaceutical Uses
No pharmacopeial status or formulation role is provided for UPCDC30766. This material is sold strictly for research use only.
General discovery context (not item-specific)
Early-phase discovery: Compounds like this are used for target validation, hit identification, and SAR development. Any transition toward pharmaceutical development would require comprehensive CMC characterization, impurity profiling, stability studies, and toxicology—none of which are claimed or provided here.
Excipient use: None indicated or implied. This listing is not intended as an excipient or API.
Regulatory note
Do not use in humans or for diagnostic procedures. For laboratory research only. For any GLP/GMP applications, contact your QA to determine suitability; this listing makes no GMP claims.
Physical Properties
Item-specific physical constants are not provided in the product data.
Appearance: Not specified for this item; refer to CoA/Spec Sheet.
Melting point, boiling point, density, refractive index: Not specified for this item; refer to CoA/Spec Sheet.
Solubility: Not specified for this item; refer to CoA/Spec Sheet.
General/literature guidance for small-molecule screening compounds (not item-specific)
Stock preparation: 10–50 mM in anhydrous DMSO is commonly used for discovery screening libraries, with working assay concentrations typically in the nM–low µM range.
Aqueous compatibility: Most drug-like molecules require a co-solvent (e.g., DMSO 0.1–1% v/v final) to avoid precipitation in buffered media.
Stability considerations: Many discovery compounds can be sensitive to hydrolysis, oxidation, or photolysis depending on functional groups. Minimize water content during stock preparation and protect from light until specific stability data are available.
Important
Treat all values above as general best practices; they are not specifications for this item. For definitive physical data, consult the batch CoA/Spec Sheet and SDS.
Quality and Grades
Grade: Moligand™ (as listed). This denotes a small‑molecule screening ligand intended for discovery workflows (biochemical/biophysical assays, phenotypic screens, fragment or hit validation), not for clinical or diagnostic use.
What this means in practice
Intended use: Hit finding, SAR exploration, and probe development. Not formulated for GMP use.
Documentation: Certificate of Analysis (CoA) and/or Spec Sheet should be consulted for lot-specific identity and quality data (e.g., analytical method used, purity basis, water content, residual solvents). If not included in the shipment, request from technical support.
Purity and stabilizers: Not specified for this item; refer to CoA/Spec Sheet. Do not assume the presence/absence of stabilizers or salts.
Chromatographic suitability: Low-UV or “HPLC grade solvent compatibility” is not applicable to the compound itself; however, library compounds are generally evaluated by LC–MS or HPLC for identity/purity. The specific method and results must be verified on the CoA.
Practical guidance
Before biological testing, verify identity/purity with your internal QC if required by your workflow.
If receiving in solution, record the exact concentration, solvent, and any additives as indicated on the label/CoA. If receiving as a solid, weigh promptly at room temperature to minimize moisture uptake, then re‑seal and return to −80°C.
Reaction and Applications
This product is positioned as a screening ligand rather than a synthetic reagent. Consequently, classical synthetic reaction applications (e.g., cross-coupling, condensations) are not typically relevant without knowing the structure.
Research applications (general, not item-specific)
Primary use: High‑throughput screening (HTS), target-based assays (enzyme, receptor, transporter), or phenotypic assays to identify activity profiles.
Orthogonal confirmation: Surface-based biophysical techniques (SPR, BLI), thermal shift assays (DSF/TSA), microscale thermophoresis (MST), and NMR-based binding are commonly used to validate hits.
Profiling: Counterscreens for pan-assay interference (PAINS), redox cycling, aggregation, and fluorescence interference should be included in assay cascades.
Plate management: Use low-binding plasticware, control DMSO percentage across plates, and maintain humidity to minimize edge effects.
If synthetic derivatization is intended
Obtain structural information and CoA first. Without structural knowledge, no guidance on chemoselectivity or protecting-group strategy can be provided.
Item-specific reaction behavior, mechanisms, or named-reaction applicability: Not specified for this item; refer to CoA/Spec Sheet if the structure is disclosed.
Reaction Conditions
Classical synthetic reaction conditions are not applicable here without the compound’s structure. For assay use, the following general, literature-based conditions may be helpful (not item-specific specifications):
Assay and handling conditions (general)
Stock solutions: 10–50 mM in anhydrous DMSO; store at −80°C in single-use aliquots to avoid freeze–thaw.
Working concentrations: Typically nM to low µM in biochemical assays; maintain constant DMSO percentage (0.1–1% v/v) across controls and treatments.
Temperature: Prepare and dispense at ambient temperature; incubate assays at temperatures required by the target (often 20–37°C).
Light/oxygen: Protect from strong light; degas buffers only if the assay is oxygen-sensitive.
Filtration: Use 0.22 µm filtration for buffer solutions; avoid filtering DMSO stocks unless necessary due to potential compound binding to membranes.
Controls and analytics
Include vehicle controls matched for DMSO percentage.
Monitor precipitation visually or by light scatter; consider orthogonal readouts if fluorescence interference is possible.
Item-specific kinetic parameters, pKa, or solubility product: Not specified for this item; refer to CoA/Spec Sheet and internal method development.
Safety and Handling
Safety data specific to UPCDC30766 are not provided in this listing.
GHS Classification, Signal Word, H‑Statements, Pictograms: Not specified for this item; refer to SDS.
Research Use: For research use only.
Storage: Store at −80°C (per product data). Maintain cold chain during handling.
General precautions for small-molecule screening compounds (not item-specific)
PPE: Use lab coat, safety glasses, and appropriate chemical-resistant gloves. Handle in a fume hood to avoid inhalation of dust or vapors.
Avoid: Skin/eye contact, ingestion, and aerosolization. Prevent cross-contamination of assay plates and neighboring compounds.
Incompatibilities: Unknown for this specific item. As a precaution, avoid strong oxidizers/reducers, strong acids/bases, and reactive metals until the structure is known.
First aid (overview): If on skin/eyes, rinse with water for several minutes; remove contaminated clothing. If inhaled, move to fresh air. If ingested, rinse mouth. Seek medical attention and provide the SDS.
Spill response: Absorb small spills with inert material (e.g., vermiculite), collect in a sealed container, and dispose according to institutional and local regulations.
Thermal/light sensitivity: Without structural information, protect from light and heat as a precaution. Keep containers tightly closed and moisture‑free.
Always defer to the SDS for authoritative safety information.
Solvent Selection
Item-specific solubility is not provided. The following are general, literature-based practices for small-molecule screening compounds (not item-specific specifications):
Primary stock solvent
DMSO (anhydrous) is the default due to broad solvency for drug-like molecules and compatibility with most biochemical/cellular assays at 0.1–1% v/v final.
Secondary solvents (when appropriate)
DMF or NMP: Similar solvency to DMSO; consider cytotoxicity at higher assay percentages.
Ethanol or isopropanol: Useful for moderately lipophilic molecules; often limited to ≤1% v/v in biological assays.
Aqueous buffers: Only for highly polar/ionizable compounds; may require pH adjustment and surfactant (e.g., 0.01–0.05% Tween‑20) to prevent aggregation (general guidance).
Miscibility and handling
Ensure solvent and buffer are pre-equilibrated to room temperature to avoid precipitation upon dilution.
Use serial pre-dilution in DMSO before introducing into aqueous buffers to keep the final DMSO percentage consistent across wells/conditions.
Selection tips
Start with 10 mM DMSO stocks; evaluate clarity upon dilution to the planned working concentration.
If precipitation occurs, explore co-solvent gradients (e.g., 0.1–1% DMSO), adjust pH within biologically acceptable ranges, or reduce compound concentration.
Note: No item-specific solubility parameters (logP, pKa, dielectric response) are available for this compound in the product data.
Storage and Reconstitution
Item-specific conditions
Storage: Store at −80°C (as provided in product data). Maintain cold chain during handling.
Appearance and form: Not specified for this item; refer to CoA/Spec Sheet.
Reconstitution (general guidance; not item-specific specifications)
Solvent: Use anhydrous DMSO for initial stock preparation unless otherwise indicated on the CoA.
Concentration: Common practice is 10–50 mM for screening stocks; adjust based on solubility tests.
Technique: Bring container to room temperature in a desiccated environment before opening to avoid moisture condensation. Weigh quickly if solid. Add solvent incrementally with gentle vortexing; brief sonication may help.
Filtration: Avoid filtering DMSO stocks unless particulate remains; if needed, use low-binding PTFE filters.
Aliquoting and stability
Prepare single-use aliquots (e.g., 10–50 µL) to minimize freeze–thaw cycles.
Store aliquots at −80°C in amber vials or foil-wrapped tubes to protect from light.
Thaw on ice or at room temperature; use immediately. Refreezing small residual volumes is discouraged.
Specifications such as exact solubility, stability in buffer, or long-term shelf life: Not specified for this item; refer to CoA/Spec Sheet and SDS.
Structure and Identity
Brief overview: UPCDC30766 is supplied as a Moligand™ small‑molecule screening compound. Structural identifiers are not disclosed in this listing.
SKU: U1498059
Product Name: UPCDC30766
CAS: 2152623-68-0
Category: Small molecule screening compound (Moligand™ series)
Molecular Formula: Not specified for this item; refer to CoA/Spec Sheet.
Molecular Weight: Not specified for this item; refer to CoA/Spec Sheet.
SMILES: Not specified for this item; refer to CoA/Spec Sheet.
InChIKey: Not specified for this item; refer to CoA/Spec Sheet.
Structure description: Not disclosed in product data. Functional groups, ring systems, and stereochemistry cannot be summarized without the structure.
Notes
This product is intended for research and screening use only; no biological target annotation is provided in the catalog entry.
For detailed identifiers and batch-specific characterization (e.g., NMR/LC–MS), consult the CoA/Spec Sheet or contact technical support.
Synthetic Utility
UPCDC30766 is offered as a screening compound, not as a general-purpose synthetic building block. Without structural disclosure, synthetic transformations and reactivity cannot be meaningfully described.
General considerations (not item-specific)
If you plan to derivatize or immobilize the compound (e.g., for SAR, pull‑downs, or probe development), request structural and analytical details first. Confirm availability of functional handles (amines, acids, halides, etc.) before planning routes.
For proteomics probes or affinity reagents, linkers (e.g., PEG, diazirine/alkyne photoaffinity tags) typically require installation at positions that preserve activity; SAR data are essential to select attachment points.
For bioconjugation, evaluate stability of the core scaffold to common coupling conditions (amide coupling, click chemistry) only after structure is known.
Item-specific reactivity, protecting-group strategy, and named reactions: Not specified for this item; refer to CoA/Spec Sheet if structure is provided.
Target Specificity
No target, epitope, or selectivity data are provided for UPCDC30766 in the product data.
Target/Pathway: Not specified for this item; refer to CoA/Spec Sheet or your internal screening results.
Species reactivity: Not applicable.
Assay-validated specificity: Not provided.
Guidance (general, not item-specific)
Establish specificity via panel screening against related targets or counterscreens to rule out off-target mechanisms and assay interference.
Employ orthogonal methods (e.g., SPR/BLI, DSF/TSA, enzymology with alternative substrates) to corroborate mechanism.
よくある質問
What is Moligand??
Moligand? is one of Aladdin Scientific's premium product lines, formulated for high-purity applications across multiple workflows. Compared to standard grades in the same workflow, Moligand? products typically offer tighter specifications, more rigorous QC, and stronger lot-to-lot consistency.
How should this product be stored?
Store at ?80 °C. Ultra-low temperature storage is required to maintain the specified shelf life.
How is this product shipped?
This product ships frozen with dry ice and cold packs. Unpack on arrival and transfer it to the storage condition stated above; handle dry ice with insulated gloves in a ventilated area.
What are the CAS number, molecular formula and molecular weight?
The CAS Number is 2152623-68-0, the molecular formula is C30H32F2N6O3S, and the molecular weight is 594.68 g/mol.
What documentation is provided?
Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.
Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
