CD45 Mouse mAb (Biotin) - Primary antibody, specific to CD45, Mouse IgG1

Cat. No.: Ab185413

GRADE & PURITY Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry

Host species

Mouse

Reactive species

Human

Isotype

Mouse IgG1

Clone Number

2D1

Conjugation

Biotin

Overview

Application:
Flow: ≤0.5 μg/test
For flow cytometric staining, the suggested use of this reagent is ≤0.5 μg per million cells in 100 µL staining volume or 100 µL of whole blood.

Specifications

Product Name

CD45 Mouse mAb (Biotin) - Primary antibody, specific to CD45, Mouse IgG1

Synonyms

CD45 Monoclonal Antibody (2D1), Biotin | Biotin Anti-Human CD45 Antibody[2D1] | Biotin Mouse Anti-Human CD45 | Biotin anti-human CD45 Antibody | T200 leukocyte common antigen antibody | T200 glycoprotein antibody | T200 antibody | Receptor-type tyrosine-p

Specifications & Purity

ExactAb™, Validated, 0.5 mg/mL

Host species

Mouse

Specificity

CD45

Immunogen

Human PBMC

Positive Control

Flow: Jurkat

Conjugation

Biotin

Grade

ExactAb™, Validated

Isotype

Mouse IgG1

Purification Method

Protein G purified

Concentration

0.5 mg/mL

Storage and Shipping

Storage

Store at 2-8°C,Protected from light,Do not freeze

Shipped In

Wet ice,Do not freeze

Stability And Storage

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

Flow Cytometry: CD45 Mouse mAb (Biotin) (Ab185413)

CD45 Mouse mAb (Biotin) (Ab185413) - Flow Cytometry
Flow cytometry analysis of CD45 expression by Jurkat cells (red) compared to an isotype control (blue). Cells were stained with CD45 Mouse mAb (Biotin) (Ab185413) at 0.1μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG Isotype Control Antibody (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.

Application

• Application: Dilution info
• Flow Cytometry:

  ≤0.5μg/test

  For flow cytometric staining, the suggested use of this reagent is ≤0.5 μg per million cells in 100 µL staining volume.;

Associated Targets(Human)

PTPRC Tchem Receptor-type tyrosine-protein phosphatase C (0 Activities)

Discover Target: PTPRC

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mechanisms of Action