Protocol of Western Blotting For High Molecular Weights
High molecular weight western blotting is a modification of the standard western blotting technique specifically designed for the detection and analysis of large molecular weight proteins.
Topic
Western blotting
Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
8 resources · protocols, FAQs & articles
BN-PAGE Protocol
Blue native polyacrylamide gel electrophoresis (BN-PAGE) is an electrophoretic technique used to separate and analyze large protein complexes. It preserves protein complexes in their native state under non-denaturing conditions by treating samples with mild detergents and negatively charged dyes ...
Immunoprecipitation Protocol for Native Proteins
Immunoprecipitation of natural proteins is a classic method for studying natural protein interactions, which relies on the specific binding between antibodies and proteins (antigens). This technique can be used to qualitatively detect proteins, determine the physiological role of proteins in ...
Methods of Lysate Preparation for Western Blotting
This method provides a recipe of lysate and buffer preparation for lysing cells or tissues for western blot analysis.
General Protocol for Western Blotting
Western blotting is a technique that uses specific antibodies to separate proteins and is widely used to analyze the expression of specific proteins in cell or tissue extracts. This protocol details the steps for performing western blotting of cell culture and tissue samples using both ...
Guidelines of Primary Antibodies for Western Blotting
Polyclonal, monoclonal, and recombinant antibodies are all suitable for western blotting. Polyclonal antibodies are a collection of many monoclonal antibodies that can vary between immunizations and batches. Polyclonal antibodies recognize multiple epitopes of an antigen and are therefore ...
Loading Controls for Western Blotting
Since the first publication describing Western blotting, this immunodetection technique has been widely used to identify specific proteins in complex mixtures extracted from cells or tissues.
Stripping and Reprobing Western Blotting Membranes
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Related topics
Techniques often explored alongside immunological experiments.