AMPK beta 1 Mouse mAb: Applications, Specificity & Usage Guide

AMPK beta 1 Mouse mAb is a monoclonal antibody raised in mouse and designed to specifically detect the beta 1 regulatory subunit of AMP-activated protein kinase (AMPK), a central regulator of cellular energy homeostasis.

Product SKU

Ab351150

Antibody Type

Mouse Monoclonal Antibody (mAb)

Formulation

PBS (Phosphate-Buffered Saline) only

Target

AMPK beta 1 subunit (regulatory subunit of AMP-activated protein kinase)

Host Species

Mouse

Supplier Reference

absea KC-45773

Biological Background: The Role of AMPK Beta 1

AMP-activated protein kinase (AMPK) is a heterotrimeric serine/threonine kinase complex that functions as a master sensor of cellular energy status. It is composed of a catalytic alpha subunit and two regulatory subunits: beta and gamma. The beta subunit, encoded by either PRKAB1 (beta 1) or PRKAB2 (beta 2), plays a critical structural role by acting as a scaffold that bridges the alpha catalytic subunit and the gamma nucleotide-binding subunit.

The beta 1 isoform (AMPK beta 1) is widely expressed across many tissue types and contributes to the regulation of fatty acid oxidation, glucose uptake, protein synthesis, and autophagy. Under conditions of energy stress — such as nutrient deprivation, hypoxia, or exercise — AMPK is activated and phosphorylates downstream substrates to restore ATP homeostasis. Because of its central role in metabolic regulation, AMPK is a key research target in the fields of diabetes, obesity, cancer metabolism, and cardiovascular disease.

Antibody Specifications and Formulation

The AMPK beta 1 Mouse mAb (SKU: Ab351150) is a monoclonal antibody produced in mouse host. The antibody is supplied exclusively in a PBS (phosphate-buffered saline) formulation, without carrier proteins or preservatives unless otherwise stated on the datasheet. Researchers should consult the product datasheet for confirmed details on antibody concentration, clone identity, isotype, and storage conditions prior to use.

The PBS-only formulation is advantageous for applications that are sensitive to additives such as BSA or sodium azide, including certain conjugation protocols, proximity ligation assays, and downstream functional experiments. It is recommended to aliquot the antibody upon receipt to avoid repeated freeze-thaw cycles, which can compromise antibody performance.

Recommended Applications and Protocol Guidance

Monoclonal antibodies targeting AMPK beta 1 are commonly used in a range of immunodetection applications. While specific validated applications for Ab351150 should be confirmed on the product datasheet, typical uses for anti-AMPK beta 1 monoclonal antibodies in the research community include:

• Western Blotting (WB): Detection of endogenous AMPK beta 1 protein in cell or tissue lysates. A band is typically expected at approximately 38 kDa, consistent with the molecular weight of the beta 1 subunit.
• Immunohistochemistry (IHC): Localization of AMPK beta 1 in formalin-fixed, paraffin-embedded (FFPE) tissue sections.
• Immunofluorescence (IF): Visualization of AMPK beta 1 distribution in fixed cell preparations.

Optimal antibody dilution, blocking conditions, and secondary antibody selection should be empirically determined for each application and experimental system. Positive and negative controls are strongly recommended to validate specificity.

Storage, Handling, and Quality Considerations

Proper storage and handling are essential to maintaining the activity and specificity of monoclonal antibodies. General best-practice guidelines for Ab351150 include:

• Store at –20 °C for long-term storage. Avoid repeated freeze-thaw cycles by preparing single-use aliquots.
• Keep on ice during experimental workflows to preserve antibody integrity.
• The PBS-only formulation means no sodium azide is present as a preservative; handle accordingly and avoid prolonged storage at 4 °C once thawed unless a preservative is added by the end user.
• Centrifuge briefly before use if any particulates are visible.

As with all research antibodies, lot-to-lot variability should be monitored. Researchers are encouraged to validate each new lot against a known positive control lysate or tissue section before committing to large-scale experiments.

Frequently asked questions

What is the formulation of AMPK beta 1 Mouse mAb (Ab351150)?

Ab351150 is supplied in PBS (phosphate-buffered saline) only. This formulation is free from carrier proteins such as BSA and typically does not contain sodium azide as a preservative. Researchers should verify full formulation details on the product datasheet.

What molecular weight should I expect for AMPK beta 1 in Western blotting?

The AMPK beta 1 subunit has a predicted molecular weight of approximately 38 kDa. Observed bands may vary slightly depending on post-translational modifications and the gel system used. Please verify expected band size on the specific product datasheet.

How should I store the AMPK beta 1 Mouse mAb to maintain its performance?

For long-term storage, keep Ab351150 at –20 °C. Aliquot the antibody into single-use volumes upon receipt to prevent repeated freeze-thaw cycles, which can degrade antibody quality. Once thawed, keep on ice during use and avoid prolonged storage at 4 °C without an appropriate preservative.

What species reactivity does AMPK beta 1 Mouse mAb have?

Species reactivity for Ab351150 should be confirmed on the official product datasheet, as validated reactivity depends on the specific clone used. Anti-AMPK beta 1 antibodies commonly show reactivity against human and mouse samples, but cross-reactivity with other species should be experimentally verified.

References

1. Hardie DG, Ross FA, Hawley SA. AMPK: a nutrient and energy sensor that maintains energy homeostasis. Nature Reviews Molecular Cell Biology. 2012;13(4):251-262. doi:10.1038/nrm3311
2. Steinberg GR, Kemp BE. AMPK in Health and Disease. Physiological Reviews. 2009;89(3):1025-1078. doi:10.1152/physrev.00011.2008
3. Aladdin Scientific. AMPK beta 1 Mouse mAb, SKU Ab351150. Product datasheet. absea KC-45773.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.