DNPEP Mouse mAb: Selection, Application & Usage Guidelines

DNPEP Mouse mAb is a high-purity mouse monoclonal antibody targeting aspartyl aminopeptidase (DNPEP), formulated at 0.95 mg/ml in PBS and designed to support accurate, reproducible detection of DNPEP across a range of standard immunological assay formats.

Product SKU

Ab377735

Antibody Format

Mouse Monoclonal Antibody (mAb)

Concentration

0.95 mg/ml

Formulation

PBS Only

Target Protein

DNPEP (Aspartyl Aminopeptidase)

Host Species

Mouse

Product Overview and Target Biology

DNPEP (Aspartyl Aminopeptidase, also known as Asp-AP) is a zinc-dependent metalloprotease belonging to the M18 family of aminopeptidases. It catalyzes the removal of N-terminal aspartate or glutamate residues from peptide substrates and has been implicated in neuropeptide metabolism, the renin-angiotensin system, and intracellular protein degradation pathways.

Aladdin Scientific's DNPEP Mouse mAb (Ab377735) is produced using established hybridoma technology, delivering the specificity and batch-to-batch consistency required for demanding research applications. The antibody is formulated at a concentration of 0.95 mg/ml in phosphate-buffered saline (PBS) without the addition of stabilizers, preservatives, or carrier proteins, making it compatible with a broad range of downstream conjugation and assay procedures.

Researchers studying aminopeptidase biology, protease substrate profiling, or the renin-angiotensin-aldosterone system (RAAS) may find this antibody valuable for characterizing endogenous DNPEP expression levels, subcellular localization, and protein–protein interactions.

Recommended Applications and Working Dilutions

Mouse monoclonal antibodies against intracellular enzymes such as DNPEP are typically suited for the following immunological techniques. Users should perform empirical titration experiments to determine the optimal working concentration for their specific cell lines, tissue types, and detection systems.

• Western Blot (WB): A starting dilution range of 1:500–1:2000 is commonly recommended for monoclonal antibodies of this format. Titrate to determine the concentration yielding the highest signal-to-noise ratio.
• Immunohistochemistry (IHC): Starting dilutions of 1:50–1:200 are typical. Antigen retrieval (heat-induced epitope retrieval in citrate buffer, pH 6.0, or EDTA buffer, pH 9.0) may be required depending on tissue fixation method.
• Immunofluorescence (IF) / Immunocytochemistry (ICC): A dilution range of 1:100–1:500 is a reasonable starting point. Optimize blocking conditions to minimize background in your specific cell system.
• Immunoprecipitation (IP): Use 1–5 µg of antibody per reaction as a starting point; optimize with respect to input lysate concentration.

Note: Validated application data specific to Ab377735 should be confirmed with Aladdin Scientific technical support, as experimental validation status may vary by application.

Storage, Handling, and Stability Guidelines

Proper storage and handling of DNPEP Mouse mAb (Ab377735) are essential to maintain antibody integrity, binding affinity, and experimental reproducibility over time. Please observe the following guidelines:

• Short-term storage (≤1 month): Store at 4°C. Avoid repeated freeze-thaw cycles, which can cause antibody aggregation and loss of activity.
• Long-term storage: Aliquot the antibody into single-use volumes appropriate for your experimental workflow and store at −20°C or −80°C. This minimizes degradation from repeated freeze-thaw cycles.
• Formulation considerations: As the product is formulated in PBS only (no glycerol, BSA, or sodium azide), users who require long-term storage stability may consider adding glycerol to a final concentration of 20–50% (v/v) to serve as a cryoprotectant. Consult Aladdin Scientific technical support before modifying the formulation if downstream conjugation or enzyme activity assays are planned.
• Avoid: Prolonged exposure to room temperature, repeated freeze-thaw cycles, and contamination from non-sterile pipettes or tubes.
• Concentration at receipt: 0.95 mg/ml — no dilution is required prior to aliquoting.

Always centrifuge the vial briefly (1,000 × g, 30 seconds) after thawing to collect any condensate before opening.

Troubleshooting Common Experimental Issues

The following troubleshooting guide addresses the most frequently encountered problems when working with mouse monoclonal antibodies targeting intracellular proteins such as DNPEP.

Problem	Possible Cause	Recommended Action
No signal detected	Antibody concentration too low; insufficient antigen expression; protein degradation in lysate	Increase antibody concentration; verify target expression in chosen cell/tissue model; add protease inhibitors during lysis
High background / non-specific bands	Primary antibody concentration too high; insufficient blocking; cross-reactivity with secondary antibody	Titrate antibody to lower concentration; increase blocking time/stringency; include a no-primary-antibody control
Weak or inconsistent signal between experiments	Freeze-thaw degradation; inconsistent sample loading	Use freshly thawed single-use aliquots; normalize protein loading with a housekeeping protein
Loss of activity after storage	Repeated freeze-thaw cycles; storage at suboptimal temperature	Prepare single-use aliquots; store at −20°C or −80°C with added cryoprotectant if required

For issues not addressed here, contact Aladdin Scientific technical support with your lot number and experimental details.

Ordering Information and Quality Assurance

DNPEP Mouse mAb is available exclusively through Aladdin Scientific under the catalog number Ab377735. The product is supplied at a concentration of 0.95 mg/ml in PBS.

Each lot of Ab377735 undergoes internal quality control testing prior to release to ensure concentration accuracy and antibody integrity. Researchers are encouraged to record the lot number at the time of receipt to facilitate any technical inquiries or repeat-order consistency checks.

Ordering options:

• Contact your regional Aladdin Scientific sales representative for volume pricing and institutional account setup.
• Purchase orders (PO number P260020464 format) can be submitted through the Aladdin Scientific online ordering portal or via email to your regional office.
• Bulk quantities and custom formulations (e.g., with added BSA, glycerol, or azide) may be available upon request — please contact technical support for feasibility discussion.

For questions regarding shipping conditions, return policies, or payment options, please refer to the relevant sections of the Aladdin Scientific Help Center or contact customer service directly.

Frequently asked questions

What is the recommended storage condition for DNPEP Mouse mAb (Ab377735)?

For short-term use within one month, store Ab377735 at 4°C. For long-term storage, aliquot the antibody into single-use volumes and store at −20°C or −80°C to avoid activity loss from repeated freeze-thaw cycles. Because the formulation contains PBS only without glycerol, consider adding glycerol (20–50% v/v) as a cryoprotectant for extended storage.

What applications is DNPEP Mouse mAb validated for?

DNPEP Mouse mAb (Ab377735) is a mouse monoclonal antibody suitable for standard immunological techniques including Western blot, immunohistochemistry, immunofluorescence, and immunoprecipitation. Users should perform empirical titration for their specific system, and confirmed application-specific validation data should be verified with Aladdin Scientific technical support.

Can DNPEP Mouse mAb be used for downstream conjugation or enzyme activity assays?

Because Ab377735 is formulated in PBS only—without BSA, glycerol, or sodium azide—it is generally compatible with conjugation chemistries and enzyme-linked assay formats. However, users planning conjugation or activity-based assays should consult Aladdin Scientific technical support before modifying the formulation or proceeding, to confirm compatibility with their specific chemistry.

What is the supplied concentration of DNPEP Mouse mAb and do I need to dilute it before aliquoting?

Ab377735 is supplied at a concentration of 0.95 mg/ml in PBS. No dilution is required prior to aliquoting. After thawing, briefly centrifuge the vial at 1,000 × g for 30 seconds to collect condensate before opening, then aliquot into volumes appropriate for your experimental needs.

How should I troubleshoot high background when using DNPEP Mouse mAb in Western blot?

High background in Western blot typically results from excessive primary antibody concentration, insufficient blocking, or cross-reactivity issues. To resolve this, titrate Ab377735 to a lower working concentration, increase the blocking duration or use a higher-concentration blocking reagent, and always include a no-primary-antibody negative control to distinguish true signal from background. Increasing wash stringency (number of washes or detergent concentration) can also help reduce non-specific signal.

References

1. Rawlings ND, Barrett AJ, Thomas PD, Huang X, Bateman A, Finn RD. The MEROPS database of proteolytic enzymes, their substrates and inhibitors in 2017 and a comparison with peptidases in the PANTHER database. Nucleic Acids Res. 2018;46(D1):D624–D632. doi:10.1093/nar/gkx1134
2. Wilk S, Wilk E, Magnusson RP. Purification, characterization, and cloning of a cytosolic aspartyl aminopeptidase. J Biol Chem. 1998;273(26):15961–15970. doi:10.1074/jbc.273.26.15961

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.