Answers from Aladdin Scientific's technical team to 290+ questions on antibodies, reagents, purity, storage and experiment troubleshooting.
Can this product be used directly for staining of adherent cells? Can it be used to stain live cells and determine apoptosis by staining?
Do I need to adjust the pH again after diluting EDTA Antigen Repair Solution?
Can DAPI be excited with laser confocal λ=405nm after staining the nucleus?
What are the temperature requirements for eluting antibodies in an antibody removal solution? Can the same membrane be eluted more than once?
Is this lysate suitable for extracting mitochondrial proteins?
Can RIPA lysis buffer continue to be used after being stored unopened at room temperature for 24 hours? Is there a limit to the number of repeated freezing and thawing?
At what temperature is the pH 7 .4 of RIPA lysis buffer measured?
Does RIPA lysis buffer contain reducing agents such as DTT or mercaptoethanol?
Do I need to add extra mercaptoethanol before using this product?
