HSP90AA4P Mouse mAb: Detection & Research Applications

HSP90AA4P Mouse mAb is a mouse-derived monoclonal antibody targeting the HSP90AA4P pseudogene-associated protein, formulated in PBS and designed to support precise detection in heat shock protein-related research workflows.

Product SKU

Ab351245

Antibody Type

Mouse Monoclonal Antibody (mAb)

Target

HSP90AA4P (Heat Shock Protein 90 Alpha Family Class A Member 4, Pseudogene)

Formulation

PBS (Phosphate-Buffered Saline)

Host Species

Mouse

Supplier Reference

absea #KC-30351

Product Overview

HSP90AA4P Mouse mAb (catalog number Ab351245) is a mouse-derived monoclonal antibody developed against the HSP90AA4P target — a pseudogene locus within the heat shock protein 90 alpha (HSP90α) gene family. The antibody is supplied in phosphate-buffered saline (PBS) without additional carrier proteins or preservatives unless otherwise stated, making it compatible with a broad range of downstream immunological applications.

The product is referenced under the absea catalog number KC-30351, and is distributed by Aladdin Scientific to support research into heat shock protein biology, chaperone networks, and pseudogene transcriptomics.

Background: HSP90 Family and Pseudogene Biology

Heat shock protein 90 (HSP90) is a highly conserved molecular chaperone essential for the folding, stabilization, and activation of numerous client proteins involved in signal transduction, cell proliferation, and stress response. The HSP90 family in humans includes several alpha and beta isoforms encoded across the genome.

Pseudogenes such as HSP90AA4P arise from ancestral gene duplication or retrotransposition events and may lack functional open reading frames. However, emerging evidence suggests that certain pseudogene loci can produce transcripts with regulatory roles, including acting as competing endogenous RNAs (ceRNAs) that modulate miRNA activity. Research tools targeting HSP90AA4P-associated sequences therefore contribute to understanding the non-coding transcriptome and its potential implications in cellular stress biology.

Recommended Applications and Usage Guidelines

While specific validated application data should be confirmed in the product datasheet, monoclonal antibodies of this class are generally evaluated for use in applications such as:

• Enzyme-Linked Immunosorbent Assay (ELISA) — for quantitative detection of target antigen in biological samples.
• Western Blot (WB) — for size-based identification of HSP90AA4P-related protein or transcript-derived peptides.
• Immunofluorescence (IF) — for subcellular localization studies in fixed cell preparations.
• Immunoprecipitation (IP) — for pull-down of target complexes from cell lysates.

Researchers should optimize primary antibody concentrations, blocking conditions, and secondary antibody pairings empirically for each application and sample type. Storage at –20 °C is recommended for long-term stability; avoid repeated freeze-thaw cycles.

Storage, Handling, and Quality Considerations

HSP90AA4P Mouse mAb is formulated in PBS, a physiologically compatible buffer that maintains antibody stability at neutral pH. Key handling recommendations include:

• Store at –20 °C upon receipt for long-term preservation.
• For short-term use (up to 1 month), the antibody may be stored at 4 °C.
• Aliquot into single-use volumes prior to freezing to minimize freeze-thaw degradation.
• Avoid exposure to repeated temperature fluctuations, direct light, or denaturing agents.

As a monoclonal antibody produced in mouse, researchers working with human-derived detection systems should select appropriate species-compatible secondary antibodies (e.g., anti-mouse IgG conjugates) to ensure signal specificity and minimize background.

Frequently asked questions

What is HSP90AA4P and why is it a research target?

HSP90AA4P is a pseudogene belonging to the HSP90 alpha gene family. Although pseudogenes were historically considered non-functional, current research indicates that pseudogene-derived transcripts can act as regulatory non-coding RNAs, making them relevant to studies of gene expression regulation and cellular stress responses.

In which applications can Ab351245 (HSP90AA4P Mouse mAb) be used?

The antibody is expected to be suitable for standard immunological applications including ELISA, Western blot, immunofluorescence, and immunoprecipitation, though researchers should consult the official product datasheet for confirmed and validated applications. Empirical optimization is recommended for each experimental context.

What formulation is HSP90AA4P Mouse mAb supplied in?

HSP90AA4P Mouse mAb (Ab351245) is supplied in PBS (phosphate-buffered saline) only, without additional stabilizers or preservatives unless specified in the product datasheet. This formulation is compatible with most downstream assay buffers and dilution protocols.

How should Ab351245 be stored to maintain optimal activity?

For long-term storage, keep the antibody at –20 °C. For routine short-term use, storage at 4 °C for up to one month is acceptable. To preserve activity, aliquot the antibody into small single-use volumes and avoid repeated freeze-thaw cycles.

References

1. Taipale M, Jarosz DF, Lindquist S. HSP90 at the hub of protein homeostasis: emerging mechanistic insights. Nature Reviews Molecular Cell Biology. 2010;11(7):515-528. doi:10.1038/nrm2918
2. Vanaja GR, Ramulu HG, Kalle AM. Overexpressed HDAC8 in cervical cancer cells shows functional redundancy of tubulin deacetylation with HDAC6. Cell Communication and Signaling. 2018.
3. Poliseno L. Pseudogenes: newly discovered players in human cancer. Science Signaling. 2012;5(242):re5. doi:10.1126/scisignal.2002858

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.