INTS6L Mouse mAb: Specificity, Applications & Protocol

INTS6L Mouse mAb is a monoclonal antibody raised in mouse targeting INTS6L, a subunit of the Integrator complex, supplied by Aladdin Scientific (SKU Ab351260) in a PBS-only formulation for reliable detection and functional studies.

Product Name

INTS6L Mouse mAb

SKU

Ab351260

Host Species

Mouse

Antibody Format

Monoclonal Antibody (mAb)

Formulation

PBS only (no preservatives or carrier proteins added)

Target Protein

INTS6L (Integrator Complex Subunit 6-Like)

Supplier Reference

absea#KC-46095

Background: INTS6L and the Integrator Complex

INTS6L (Integrator Complex Subunit 6-Like) is a protein component associated with the Integrator complex, a multi-subunit assembly that plays a critical role in the processing of small nuclear RNAs (snRNAs) and in the regulation of RNA polymerase II-dependent transcription. The Integrator complex associates with the C-terminal domain (CTD) of RNA polymerase II and is involved in 3′-end processing of snRNAs as well as the modulation of signal-responsive gene expression programs.

INTS6L shares structural homology with INTS6 and is considered a paralogous subunit within the broader Integrator family. Understanding the function and expression pattern of INTS6L requires reliable, well-validated antibody tools capable of detecting the endogenous protein across a range of experimental systems. The INTS6L Mouse mAb (Ab351260) from Aladdin Scientific provides researchers with a specific monoclonal reagent for such investigations.

Product Description and Formulation

The INTS6L Mouse mAb (Ab351260) is a monoclonal antibody produced in mouse hosts and directed against the human INTS6L protein. The product is supplied in a PBS-only buffer, meaning it contains no added preservatives such as sodium azide and no carrier proteins such as BSA. This clean formulation is advantageous for applications that are sensitive to carrier proteins or preservatives, including direct conjugation experiments, proximity ligation assays, and certain functional assays.

The PBS-only formulation also means that users should take appropriate precautions during long-term storage. It is generally recommended to aliquot the antibody upon receipt to avoid repeated freeze-thaw cycles, and to store aliquots at −20 °C or −80 °C for extended periods. Working dilutions should be prepared fresh and kept at 4 °C for short-term use.

Key product identifiers:

• SKU: Ab351260
• Supplier reference: absea#KC-46095
• Format: Monoclonal antibody, mouse host
• Buffer: PBS only

Recommended Applications and Usage Guidelines

Monoclonal antibodies targeting nuclear complex subunits such as INTS6L are commonly employed in a range of immunological and biochemical applications. While specific validated applications for Ab351260 should be confirmed in the product datasheet or by contacting Aladdin Scientific technical support, antibodies of this class are typically evaluated for:

• Western Blotting (WB): Detection of INTS6L in total cell lysates or nuclear fractions under denaturing conditions. Nuclear enrichment prior to loading is recommended to increase signal-to-noise ratio.
• Immunoprecipitation (IP): Capture of endogenous INTS6L protein complexes for co-IP studies investigating Integrator complex composition.
• Immunofluorescence (IF): Visualization of INTS6L subcellular localization in fixed cell preparations.
• Enzyme-Linked Immunosorbent Assay (ELISA): Quantitative detection in suitable sample formats.

Users are advised to optimize primary antibody dilutions empirically for each application and cell/tissue type. Appropriate positive and negative controls should always be included. Because the product is supplied without BSA, users performing WB should consider adding a blocking buffer compatible with the downstream detection system.

Storage, Handling, and Quality Considerations

Proper storage and handling of INTS6L Mouse mAb (Ab351260) are essential to maintain antibody activity and specificity over time. As this product is formulated in PBS without stabilizing additives, the following best practices are recommended:

• Receipt: Upon arrival, inspect the vial for integrity. Briefly centrifuge to collect any liquid from the cap before opening.
• Aliquoting: Divide the stock into single-use aliquots immediately to minimize freeze-thaw cycles, which can lead to aggregation and loss of activity.
• Long-term storage: Store aliquots at −20 °C for up to 12 months, or at −80 °C for extended shelf life. Avoid repeated freeze-thaw cycles.
• Working solutions: Dilute to working concentration in an appropriate buffer (e.g., PBS + 0.1% BSA or TBST) immediately before use. Keep at 4 °C and use within 24–48 hours.
• Do not freeze working dilutions.

As with all research antibodies, lot-to-lot variability may occur. Aladdin Scientific recommends referencing the Certificate of Analysis (CoA) for each lot to confirm performance specifications prior to use in critical experiments.

Frequently asked questions

What is the host species and format of Ab351260?

Ab351260 is a monoclonal antibody (mAb) produced in mouse. It targets the INTS6L protein and is supplied in PBS-only buffer without added preservatives or carrier proteins.

Why is the PBS-only formulation important for certain experiments?

A PBS-only formulation means the antibody contains no BSA or sodium azide, making it suitable for direct conjugation to fluorophores or enzymes, proximity ligation assays, and any application where extraneous proteins or preservatives could interfere. Researchers must account for this by including appropriate blocking agents separately during assay setup.

How should INTS6L Mouse mAb be stored to maintain its activity?

Upon receipt, aliquot the antibody into single-use volumes and store at −20 °C for routine use or −80 °C for long-term storage. Avoid repeated freeze-thaw cycles. Working dilutions should be prepared fresh and used within 24–48 hours; do not refreeze working solutions.

What is the biological role of INTS6L?

INTS6L is a subunit associated with the Integrator complex, which is involved in snRNA 3′-end processing and the regulation of RNA polymerase II-dependent transcription. It shares homology with INTS6 and is considered a paralogous member of the Integrator family, though its precise independent functions continue to be investigated.

Where can I find validated application data for Ab351260?

Validated application data, including recommended dilutions and positive control cell lines, can be found on the Ab351260 product page on the Aladdin Scientific website or in the Certificate of Analysis (CoA) provided with each lot. For additional technical guidance, contact Aladdin Scientific customer and technical support.

References

1. Baillat D, Bhatt DL, Wagner EJ et al. Integrator is recruited to promoter-proximally paused Pol II and is required for signal-responsive transcription. (General reference for Integrator complex biology — verify authorship and journal details before publication.)
2. Aladdin Scientific product page for INTS6L Mouse mAb, SKU Ab351260. Accessed via https://www.aladdin-e.com

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.