PDE5A Mouse mAb: FAQs for Research Use

PDE5A Mouse mAb is a mouse monoclonal antibody targeting phosphodiesterase 5A, supplied in PBS formulation for use in research applications requiring specific detection of the PDE5A protein.

Product SKU

Ab350618

Antibody Type

Mouse Monoclonal Antibody (mAb)

Formulation

PBS (Phosphate-Buffered Saline) only

Target Protein

PDE5A (Phosphodiesterase 5A)

Source Organism

Mouse

Supplier Reference

absea #KC-207

What Is PDE5A and Why Is It Studied?

Phosphodiesterase 5A (PDE5A) is a cyclic nucleotide phosphodiesterase that specifically hydrolyzes cyclic guanosine monophosphate (cGMP) to 5′-GMP. By regulating intracellular cGMP levels, PDE5A plays a central role in smooth muscle relaxation, cardiovascular physiology, and cellular signaling pathways downstream of nitric oxide (NO). Research on PDE5A is relevant to areas including pulmonary arterial hypertension, erectile dysfunction, cardiac hypertrophy, and cancer biology. Selective inhibitors of PDE5A (such as sildenafil) are established therapeutics, making reliable antibody tools essential for mechanistic studies.

Product Description and Formulation Details

PDE5A Mouse mAb (SKU: Ab350618) is produced using mouse hybridoma technology to yield a monoclonal antibody with defined specificity against the PDE5A antigen. The antibody is referenced under the supplier code absea #KC-207. The product is formulated exclusively in PBS (phosphate-buffered saline) without additional stabilizers such as glycerol, BSA, or sodium azide unless specified at time of ordering. Researchers should confirm exact buffer composition, concentration, and any preservatives with Aladdin Scientific's technical support prior to use, as lot-specific certificates of analysis may contain additional detail.

Recommended Applications and Usage Guidelines

Monoclonal antibodies against PDE5A are commonly employed in a range of immunological and biochemical assays. Typical applications for antibodies of this class include Western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry (IHC), and enzyme-linked immunosorbent assay (ELISA). Because validated application data specific to Ab350618 are not fully detailed in the current source material, researchers are strongly advised to consult the product datasheet or contact Aladdin Scientific technical support for confirmed application compatibility, recommended dilution ranges, and positive control cell lines or tissues.

Storage, Handling, and Stability

Antibodies formulated in PBS without glycerol are generally more susceptible to freeze-thaw degradation than those containing cryoprotectants. For short-term storage (up to 4 weeks), keeping the antibody at 4 °C is typically acceptable. For long-term storage, aliquoting the antibody into single-use volumes and storing at −20 °C or −80 °C is recommended to minimize repeated freeze-thaw cycles. Avoid exposure to repeated temperature fluctuations, direct light, and contamination. Because specific stability data for Ab350618 are not provided in the source material, consult the product datasheet or certificate of analysis for validated storage conditions and expiry information.

Ordering and Technical Support

PDE5A Mouse mAb (Ab350618) is available for order directly through Aladdin Scientific. When placing an order, researchers should specify any requirements regarding formulation adjustments, concentration, or custom packaging. For technical questions regarding antibody validation, application-specific protocols, or troubleshooting, contact Aladdin Scientific's technical support team. Bulk or custom orders, including requests for additional quality documentation such as lot-specific ELISA or Western blot validation data, can also be accommodated upon request. Pricing, availability, and lead times are available on the Aladdin Scientific website or via the sales team.

Frequently asked questions

What is the formulation of PDE5A Mouse mAb (Ab350618)?

PDE5A Mouse mAb (Ab350618) is formulated in PBS (phosphate-buffered saline) only, as indicated by the product description. It does not contain glycerol or other cryoprotectants unless otherwise specified. Researchers should verify the exact composition including any preservatives on the lot-specific certificate of analysis.

What applications is PDE5A Mouse mAb validated for?

Specific validated application data for Ab350618 are not fully detailed in the available source material. Antibodies of this class are commonly used in Western blotting, immunoprecipitation, immunofluorescence, and immunohistochemistry. Please contact Aladdin Scientific technical support or refer to the product datasheet for confirmed application compatibility and recommended dilutions.

How should PDE5A Mouse mAb be stored to maintain activity?

Because the antibody is formulated in PBS without cryoprotectants, it is recommended to aliquot the antibody into single-use volumes immediately upon receipt and store at −20 °C or −80 °C for long-term use. For short-term use within 4 weeks, storage at 4 °C is generally acceptable. Avoid repeated freeze-thaw cycles, as these can reduce antibody activity.

What is the supplier reference code for Ab350618?

PDE5A Mouse mAb (Ab350618) is referenced under the supplier code absea #KC-207. This code may be useful when cross-referencing technical documentation or communicating with the manufacturer for additional product information.

Can PDE5A Mouse mAb be used for human and mouse samples?

Species cross-reactivity information for Ab350618 is not specified in the available source material. Researchers should consult the product datasheet or contact Aladdin Scientific technical support to confirm which species the antibody has been validated against before use in experiments.

References

1. Aladdin Scientific product listing: PDE5A Mouse mAb, SKU Ab350618, absea #KC-207, PBS formulation.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.