Purification experiments for mucoid and plasmid clones
The library should be amplified as soon as possible once it is packaged; it can greatly increase the copy number of the library, but there may be some potential
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Experiments on stable transfection of mammalian cell genes
Analyzing the function of a gene often requires the formation of a mammalian cell line containing the stably integrated gene. In transfection, approximately 1/1
Experiments with the Hanahan method for the preparation and transformation of susceptible Escherichia coli (efficient transformation strategies)
In the late 1970s and early 1980s, when Dong Hanahan was a graduate student at Cold Spring Harbor Laboratory and Harvard University, he made previously unheard
Experimenting with connectors on protruding ends
The source of this experiment is "Guide to Molecular Cloning Experiments, Third Edition", translated by Huang Peitang et al.
In vitro transcription and translation experiments of cloned genes
DNA cloning refers to the process of molecular manipulation in which a DNA fragment containing a target gene or other meaningful DNA is ligated in vitro with a
Targeted cloning experiments in plasmid vectors
Most commonly used plasmids contain multiple cloning sites that can be recognized by different endonucleases. Because of the large number of cloning sites avail
Phage-spreading plate assay for phage spot production
Source of content: compact guide to molecular biology 5th ed.
Sephacryl S-1000 Chromatography for Removal of Small Fragments of Nucleic Acids from Plasmid DNA Preparations
Sephacryl S-1000 chromatography is an alternative method for separating plasmid DNA from small molecules of nucleic acids (DNA and RNA). This method was first u
Dephosphorylation experiments on enzyme section breaks
In the modification of DNA fragments, the dephosphorylation reaction is an important element, which is catalyzed by alkaline phosphatase, an enzyme that removes