T4 DNA polymerase assay
T4 DNA polymerase has a human template-dependent polymerase activity, as well as exonuclease activity for the 3' to 5' ends of single- and double-stranded DNA,
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Determination of DNA by chemical method - Diphenylamine colorimetric method
Chemical determination of DNA content can be used for (1) estimation of plasmid concentration prior to transformation and transfection, and (2) assay of acquire
Experiments on preparation of DNA probes labeled with potenticin
The digoxin system is over provided as another non-isotopic labeling method, which is detected by coupling an anti-digoxin antibody to one or more fluorescent d
Freeze-thaw method for recovery of DNA fragments
The freeze-thaw method of recovering DNA fragments can be used to (1) obtain purified DNA fragments and (2) recover PCR products.
Removal of ethidium bromide from DNA by ion exchange chromatography
Ethidium bromide in DNA purified by cesium chloride gradient centrifugation can be removed by ion-exchange chromatography, followed by ethanol precipitation to
Plasmid DNA preparation by the toothpick method in small quantities
Colonies picked with a toothpick from agar medium can be used directly to prepare plasmid DNA. the resulting closed-loop DNA often has too many impurities to be
Chemiluminescent Dideoxy DNA Sequencing Assay
Standard double deoxy DNA sequencing can be easily and efficiently detected using non-isobaric labeled chemiluminescence. In the sequencing reaction, biotin-lab
Removal of small fragments of nucleic acids from plasmid DNA preparations by NaCl centrifugation
Conventional plasmid DNA preparations are contaminated, to varying degrees, with small molecules of DNA or RNA from the genome of the host bacterium or plasmid
Transfection of eukaryotic cells using DEAE-glucan
Mouse L-type fibroblasts were used to perform the conditions eubiquitously, but can be applied to almost any cell with only minor modifications. It is critical