Reverse transcription and amplification experiments with low abundance nucleic acids
This method uses only one recombinant enzyme, rTth DNA polymerase, which allows for simultaneous reverse transcription and amplification in a single reaction, t
Knowledge Base
Laboratory Protocols & SOPs Library
3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
3228 articles
Antiserum potency assay by two-way immunodiffusion method
The two-way immunodiffusion method can be used to (1) diagnose disease and (2) determine the potency of antibodies.
one-way immunodiffusion assay
A single immunodiffusion assay can be used to: measure IgG, IgA and IgM levels in the serum of normal people or patients.
Hemolytic vacuole formation test
The hemolytic vacuole formation test is an in vitro method for the detection of individual antibody-forming cells (plasma cells), and is therefore also known as
Immunofluorescence complement assay
This experimental method was obtained from the official website of the Fourth Military Medical University
Experiments on the preparation of immune serum
The preparation of immune serum is a commonly used immunological laboratory technique. Highly efficient and specific immune serum can be used as a reagent for i
Pregnancy test
Pregnant women's urine to be tested (containing human chorionic gonadotropin, HCG) is interacted with known anti-HCG antibodies, thus inhibiting the binding of
Phagocytosis assay for phagocytosis
Intraperitoneal injection of sodium thioglycolate in mice can stimulate the aggregation of macrophages. Four days later, mice were injected intraperitoneally wi
B. Lymphocyte membrane surface immunoglobulin examination assay
The test for the examination of immunoglobulins on the membrane surface of B-lymphocytes can be used to: detect the presence of B-lymphocytes.