Experiments on cyclic precipitation reactions
The cyclic precipitation reaction is widely used in forensic medicine for the identification of bloodstains and the determination of food adulteration. Usually
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two-way immunodiffusion assay (BIDA)
The diffusion of antigen and antibody in a gel and the precipitation reaction is called immunodiffusion. An antigen and its corresponding antibody are placed in
Preparation of polyclonal antibodies against phosphopeptides.
It is possible to prepare anti-phosphopeptide antibodies (i.e., antibodies that recognize phosphorylated peptides) that recognize only proteins in the phosphory
Calcium staining assay
Calcium is deposited in bone tissue or pathologically altered tissue, most often in calcified tissue such as tissue necrosis, atherosclerosis and tumors. In the
IHC sensitization test
There are several ways to increase the sensitivity of IHC. One is to rely on various IHC pre-treatments, such as enzyme digestion and antigen repair at high tem
Experiments on the preparation of antigens and immune sera
Injecting the antigen into the animal body can stimulate the animal's B lymphocytes to transform into plasma cells and produce specific antibodies. When a large
Electron microscopy enzyme cytochemistry experiment
Currently there are three main classes of enzymes that can be localized by electron microscopy; they are hydrolytic enzymes, oxidative enzymes, and transferase
Perfusion fixation experiments
Source: Practical Experimental Techniques in Neurobiology, Fourth Military Medical University Press
Immunocytochemistry ICC
Localized detection of antigen expression