Reverse genetics of influenza viruses
It mainly describes the methods used to recover infectious influenza viruses from cDNA clones. These techniques are widely used in the integration and expressio
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3228 articles
Cyclodextrin-containing polycations for nucleic acid delivery
A large number of non-viral systems have been used for nucleic acid delivery to cultured cells in vitro or to specific cells in vivo. They vary considerably in
Gene gun technology for delivering genes into the skin
Gene gun technology is a fast and simple technique for delivering genes into cells. This technology has many advantages: it allows direct delivery of plasmids w
Preservation and amplification of targeted modified measles viruses
The S T A R System (6 Histidine Labeling and Retargeting) employs a pseudo-receptor and a label for a virus to enable the rescue and amplification of fully reco
Non-viral gene transfer experiments with Trojan horse liposomes that can cross the blood-brain barrier
Non-viral vector plasmid DNA can be delivered to the human brain via the vascular route across the blood-brain barrier by intravenous injection of DNA encapsula
Experiments on Prokaryotic Microinjection Technique
A technique for preparing transgenic mice by prokaryotic microinjection is described. Prokaryotic microinjection, which involves the injection of DNA into haplo
Experiments with biodegradable nanoparticles
Biodegradable nanoparticles are colloidal particles, typically around IOOnm in diameter, which can be prepared from biodegradable polymers such as polylactic ac
Down-regulation of genes generated by silencing lentiviral vectors in mice.
This chapter describes the use of lentiviral vectors to transfer genes and small interfering RNA (siR N A ) expression cassettes to preimplantation crystalline
Experiments on mifepristone-induced gene regulatory systems
The mifepristone-induced gene regulatory system (M I G R S ) is a system for regulating rake gene expression through the antiprogesterone drug, mifepristone (M