RIP technology
Studying RNA-protein binding in cells
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Laboratory Protocols & SOPs Library
3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
3228 articles
Determination of protein concentration by BCA method
Determination of protein concentration in samples
Total protein extraction from adipose tissue and cells
Extraction of total protein from adipose tissue and cells
Experiments on lysis of recombinant proteins of Escherichia coli from inclusion bodies
Source: Laboratory Guide to Proteins and Proteomics
Recombinant MVA in vivo immunostaining assay
In vivo immunostaining can be used for the detection of modified poxvirus Ankara (MVA) and also for the identification of proteins expressed on the cell surface
Experiments on bulk extraction of recombinant proteins from bacteria
Source: Laboratory Guide to Proteins and Proteomics
Far Western analysis of protein interaction experiments
When analyzing protein interactions using Far Western blot, the target protein is immobilized on a solid support membrane and then probed with a non-antibody pr
Measurement of extinction coefficients (Scopes method)
Scopes published a simple method for determining the extinction coefficient (E) of proteins (Scopes, 1974). This method is based on the fact that although most
Dialysis for the removal of sodium N-dodecyl sarcosinate
This experiment describes how much time it takes to remove sodium N- dodecyl sarcosinate by dialysis. This experiment is from the Laboratory Guide for Protein P