Vesicular stomatitis virus G protein binding assay
The capsid fusion G protein of vesicular stomatitis virus (VSV-G) can be used to prepare pseudotyped retroviruses and pseudolentiviruses, and this glycoprotein
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Laboratory Protocols & SOPs Library
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3228 articles
Transposon-mediated delivery of small interfering RNAs: the "Sleeping Beauty" transposon experiment
A method for efficient delivery of pol III-driven SHRNA expression frames using the Sleeping Beauty (SB) transposon. Advantages of this system include its ease
Linear polyethyleneimine: synthesis and in vitro and in vivo transfection manipulation experiments
Linear polyethyleneimine (L P E I ) is a very effective transfection reagent suitable for large numbers of cell lines and primary cells, and it is equally suita
Cell penetrating peptide-mediated peptide nucleic acid oligomer delivery assay
Site-specific knockout and non-toxic DNA delivery technologies are particularly important in the study of protein function and as a means for future therapies.
Reassembly experiments of human artificial chromosomes based on bacterial artificial chromosomes
Successful gene transfer requires efficient transport and long-term presence of exogenous genes in order to maintain physiologically relevant and appropriately
Experiments on a highly sensitive gene regulatory system based on a synthetic small-molecule ligand-conducted decidual hormone receptor
The RheoSwitch system is an efficient gene regulatory system based on the ecdysone receptor, which is activated by synthetic small molecule ligands. The recepto
Efficient DNA delivery by phage into mammalian cells
Transferring genes into cells and expressing new proteins, or in this way altering the expression levels of endogenous proteins, can modulate the physiological
Expression and characterization of ribozymes and short hairpin RNA in mammalian cells
Functional genomic research using R N A interference techniques (R N A i ) has been carried out extensively in recent years.R N A i is the newest and most succe
Sonopuncture: an effective technique for introducing genes into chicken embryos
The acoustic pore effect may not be applicable to the introduction of genes into tissues with lumen-forming or open structures. Acoustic pore effects on neural