Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
In vitro cytotoxicity testing is a fundamental technique in drug screening, compound safety evaluation, biomaterial assessment, and cancer research. These assays use cells as models to evaluate the effects of test substances on cell proliferation, viability, morphology, or function. Compared to ...
In the process of nuclear protein extraction, the use of surfactants can significantly interfere with protein labeling efficiency. This is because surfactants can alter the conformation of proteins and may interact with the target protein, affecting its biological activity and the accuracy of ...
For western blotting, incubate the membrane with primary antibody diluted in 1×TBST (0.1% Tween®20) containing 5% w/v BSA or nonfat dry milk overnight at 4°C on a shaker. Please refer to the primary antibody product webpage for recommended primary antibody dilution buffer and antibody dilution.
Western blotting is a technique that uses specific antibodies to separate proteins and is widely used to analyze the expression of specific proteins in cell or tissue extracts. This protocol details the steps for performing western blotting of cell culture and tissue samples using both ...
Plant genetic transformation technology, also called plant transgenic technology, is a key technique in modern biotechnology. It can improve plant genetic characteristics by introducing foreign genes into the plant genome. This article focuses on agrobacterium mediated method the efficiency and ...
In order to facilitate the experiments of scientific researchers, we have sorted out the buffer formulations commonly used in laboratory basic research in molecular biology, protein biology, microbiology and other aspects for researchers to facilitate access and use.
Techniques often explored alongside immunological experiments.
