GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.0.1 mg/mL
ExactAb™, Validated, Recombinant, 0.1 mg/mL ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Rabbit anti Human eIF5A Antibody, Recombinant, could be used for WB, IHC and so on.
Application:
WB: 1/500-1/1000 IHC: 1/50-1/100
IF/ICC: 1/200
Protein Function：
mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. With syntenin SDCBP, functions as a regulator of p53/TP53 and p53/TP53-dependent apoptosis. Regulates also TNF-alpha-mediated apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation. Also described as a cellular cofactor of human T-cell leukemia virus type I (HTLV-1) Rex protein and of human immunodeficiency virus type 1 (HIV-1) Rev protein, essential for mRNA export of retroviral transcripts.
Specifications
Product Name
Recombinant eIF5A Antibody - Primary antibody, specific to eIF5A, Rabbit IgG
A synthetic peptide derived from human eIF5A (AA 1-19)
Positive Control
WB: Jurkat, THP-1, HeLa, Raji, Hep G2, HEK293, NIH/3T3 and C6 cell lysates. IHC: Human breast cancer tissue. IF/ICC: HeLa cells.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Antigen affinity purified
Concentration
0.1 mg/mL
Note
This product is about to be sold out and removed from the shelves. The alternative product is Ab101422.
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Recombinant eIF5A Antibody (Ab101423) - Western Blot All lanes: Recombinant eIF5A Antibody (Ab101423) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 17 kDa Observed band size: 17 kDa Exposure time: 40.0 s
Recombinant eIF5A Antibody (Ab101423) - IHC Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Recombinant eIF5A Antibody (Ab101423). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Recombinant eIF5A Antibody (Ab101423) - IF/ICC IF analysis of Recombinant eIF5A Antibody (Ab101423) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant eIF5A Antibody (Ab101423) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue).
Application
Application
Dilution info
IF/ICC
1/200
WB
1/500-1/1000
IHC
1/50-1/100
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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