Donkey Anti-Rabbit IgG H&L (AF647) - Secondary antibody, specific to , IgG

Cat. No.: Ab136882

GRADE & PURITY Validated, ExactAb™, Azide Free, Ex:650nm, Em:668nm, 2.0mg/ml

Application

Flow Cytometry, IF/ICC

Host species

Donkey

Reactive species

Rabbit

Isotype

Donkey IgG

Conjugation

AF647

Overview

Donkey Anti-Rabbit IgG H&L (AF647), Secondary antibody, could be used for Flow, IF, ICC and so on.

Specifications

Product Name

Donkey Anti-Rabbit IgG H&L (AF647) - Secondary antibody, specific to , IgG

Synonyms

Donkey Anti-Rabbit IgG H&L / AF647 | Immunoglobulin G | Donkey Anti-Rabbit IgG | Donkey Anti Rabbit IgG

Specifications & Purity

ExactAb™, Validated, Azide Free, Ex:650nm, Em:668nm, 2.0mg/ml

Host species

Donkey

Immunogen

Native rabbit IgG

Positive Control

IF/ICC: Mouse cerebellum; Mouse brain; Human brain; Rat cerebellum; Rat brain

Conjugation

AF647

Excitation (nm)

Red Laser(633nm)

Emission(nm)

668nm

Ex/Em(nm)

Ex:650nm;Em:668nm

Grade

Azide Free, Em:668nm, Ex:650nm, ExactAb™, Validated

Isotype

IgG

Purification Method

Protein G purified

Concentration

2.0mg/ml

Storage and Shipping

Shape

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles

Images

IF: Donkey Anti-Rabbit IgG H&L (AF647) (Ab136882)

Donkey Anti-Rabbit IgG H&L (AF647) (Ab136882) - IF
IF analysis of HeLa cells labeling FXR1 with purified Recombinant FXR1 Antibody (Ab104281) at 1/1000 dilution. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were counterstained with beta Tubulin Mouse mAb (Ab155823) at 1/2000 dilution - Goat Anti-Mouse IgG H&L (AF488) 1/1000. Donkey Anti-Rabbit IgG H&L (AF647) (Ab136882) was used as the secondary antibody at 1/1000 dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control. Images were taken on the confocal laser scanning microscope.

IHC: Donkey Anti-Rabbit IgG H&L (AF647) (Ab136882)

Donkey Anti-Rabbit IgG H&L (AF647) (Ab136882) - IHC
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum). Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min.Blocking buffer (normal goat serum) at 37°C for 30min. Antibody incubation with (GFAP) polyclonal antibody at 1/200 overnight at 4°C, followed by a conjugated Donkey Anti-Rabbit IgG H&L (AF647) (Ab136882) for 90 minutes, and DAPI for nuclei staining.

Application

• Application: Dilution info
• Flow Cytometry:

  1/100-1/1000 ;

• IF/ICC:

  1/100-1/1000 ;