Recombinant Fbx32 Antibody - Primary antibody, specific to Fbx32

Cat. No.: Ab103063

GRADE & PURITY Recombinant, Validated, ExactAb™, See COA

Application

IF/ICC, WB

Host species

Rabbit

Reactive species

Human, Mouse, Rat

Isotype

Rabbit IgG

Conjugation

Unconjugated

Overview

Rabbit anti Human Fbx32 Antibody, Recombinant, could be used for WB, IF/ICC and so on.

Specifications

Product Name

Recombinant Fbx32 Antibody - Primary antibody, specific to Fbx32

Synonyms

FBXO32 | Fbx32 | MAFbx | 4833442G10Rik antibody | AI430017 antibody | Atrogin 1 antibody | Atrogin-1 antibody | ATROGIN1 antibody | Atrophy gene 1 antibody | F box only protein 32 antibody | F-box only protein 32 antibody | F-box protein 32 antibody | FBX

Specifications & Purity

Recombinant, ExactAb™, Validated, See COA

Biochemical and Physiological Mechanisms

Substrate recognition component of a (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins

Host species

Rabbit

Specificity

Fbx32

Immunogen

A synthetic peptide derived from human FBXO32 (AA 20-84)

Positive Control

WB: Mouse skeletal muscle, Rat skeletal muscle lysates. IF/ICC: HeLa and A673 cells.

Conjugation

Unconjugated

Grade

ExactAb™, Recombinant, Validated

Light Chain Type

kappa

SDS-PAGE

150 kDa

Purification Method

Protein A purified

Concentration

See COA

Storage and Shipping

Shape

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: Recombinant Fbx32 Antibody (Ab103063)

Recombinant Fbx32 Antibody (Ab103063) - Western Blot
All lanes: Recombinant Fbx32 Antibody (Ab103063) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 42 kDa
Observed band size: 41 kDa
Exposure time: 14.7 s

IF/ICC: Recombinant Fbx32 Antibody (Ab103063)

Recombinant Fbx32 Antibody (Ab103063) - IF/ICC
Immunofluorescent analysis of HeLa cells, using Recombinant Fbx32 Antibody (Ab103063) at 1/50 dilution.

IF/ICC: Recombinant Fbx32 Antibody (Ab103063)

Recombinant Fbx32 Antibody (Ab103063) - IF/ICC
Immunofluorescent analysis of HeLa cells, using Recombinant Fbx32 Antibody (Ab103063) at 1/150 dilution.

IF/ICC: Recombinant Fbx32 Antibody (Ab103063)

Recombinant Fbx32 Antibody (Ab103063) - IF/ICC
Immunofluorescent analysis of A673 cells, using Recombinant Fbx32 Antibody (Ab103063) at 1/50 dilution.

IF/ICC: Recombinant Fbx32 Antibody (Ab103063)

Recombinant Fbx32 Antibody (Ab103063) - IF/ICC
Immunofluorescent analysis of A673 cells, using Recombinant Fbx32 Antibody (Ab103063) at 1/150 dilution.

Application

• Application: Dilution info
• WB:

  1/1000 - 1/2000

• IF/ICC:

  1/50 - 1/200;

Associated Targets(Human)

FBXO32 Tbio F-box only protein 32 (0 Activities)

Discover Target: FBXO32

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Frequently Asked Questions

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant Fbx32 Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.