Staphylococcus Medium 110 - BioReagent

Cat. No.: S1528434

GRADE & PURITY BioReagent, Suitable for microbiology

Synonyms

Chapman Agar | Staphylococcus Agar No.110

Storage

Protected from light,Room temperature

Shipped In

Normal

Application

1131

Overview

Staphylococcus Medium 110 contains characteristic substrates such as mannitol and gelatin. It is suitable for the isolation and preliminary biochemical differentiation of staphylococci. Strains can be differentiated by mannitol fermentation and gelatin liquefaction tests. It is a selective medium for the screening and presumptive identification of staphylococci.

Medium Formula (per liter)：

Component	Content
Pancreatic Digest of Casein	10.0g
Yeast Extract	2.5g
Gelatin	30.0g
Lactose	2.0g
D-Mannitol	10.0g
Sodium Chloride	75.0g
Dipotassium Hydrogen Phosphate	5.0g
Agar	15.0g

Instructions for Use:

Weigh 149.5 g of the product into 1 L of purified water, stir and heat to boiling until fully dissolved, dispense, autoclave at 121 °C for 15 minutes, and set aside for use.

Precautions:

1. pH 7.0±0.2 at 25 °C.

2. Avoid direct contact with skin and eyes during handling. In case of accidental contact, rinse immediately with plenty of clean water.

3. This product is for laboratory research only and is not intended for clinical diagnosis.

Specifications

Synonyms

Chapman Agar | Staphylococcus Agar No.110

Specifications & Purity

BioReagent, Suitable for microbiology

Stability And Storage

Store at room temperature long term (36 months).Store in the dark.

Storage

Protected from light,Room temperature

Shipped In

Normal

Grade

BioReagent, Suitable for microbiology

Product Line

biological_products

Chemical and Physical Properties

• Sensitivity: Light-sensitive

Application Protocols

The exact preparation factor and formulation for Staphylococcus Medium 110 are not specified for this item; consult the CoA/Spec Sheet. The following protocol outlines general, literature‑based practices for preparing and using selective media for staphylococci.

A. Preparation (general)

• Weigh the dehydrated medium per the label’s grams per liter (not specified here).
• Add to ~80–90% of the final volume of purified water; mix to wet all particles.
• Heat with stirring to dissolve completely. Do not overboil.
• Adjust volume to final; verify pH if required by the formulation.
• Sterilize by autoclaving at 121 °C for 15 minutes.
• For plates, cool to 45–50 °C and pour into sterile Petri dishes. Allow to solidify and dry surface condensation before use.

B. Use (general)

• Inoculate by streaking for isolation or spreading a measured volume for enumeration.
• Incubate plates inverted at 35–37 °C for 24–48 h (literature).
• Examine for colony growth consistent with Staphylococcus phenotypes per your lab’s SOPs; confirm identity with secondary tests.

C. QC checks (general)

• Include a known Staphylococcus strain as a positive control and a non‑staphylococcal organism as a negative control to verify selectivity.

Dispose of used media by autoclaving or according to institutional biosafety procedures.

Biological Roles

The product itself has no intrinsic biological role; it is a formulated substrate that supports microbial growth. In research, Staphylococcus‑selective media exploit physiological traits of staphylococci (literature):

• Osmotolerance/halotolerance: Many Staphylococcus species tolerate elevated ionic strength and can outcompete less tolerant flora under selective conditions.
• Nutrient utilization: Complex nitrogen sources and carbohydrates (when present in a given formulation) support robust growth and can enable differential colony appearances through metabolic by‑products or indicator reactions (formulation‑dependent; not specified for this item).
• Respiratory/fermentative flexibility: Colony morphology, pigmentation, and sheen can differ between species and strains under aerobic incubation on selective media.

Use context

• Enables isolation, propagation, and preliminary phenotypic characterization of Staphylococcus spp. prior to confirmatory identification using biochemical or molecular assays, strictly in a research setting.

Caveats

• Non‑target organisms with similar tolerances may grow; media performance is influenced by incubation temperature, atmosphere, and inoculum.

For exact differentiation mechanisms (e.g., specific inhibitors/indicators), consult the CoA/Spec Sheet for this SKU, as composition is not specified here.

Buffer Applications

Not typically applicable. Staphylococcus Medium 110 is a selective culture medium, not a defined buffering system. While prepared media possess some buffering capacity inherent to their components, they are not used as standardized laboratory buffers. For buffering needs (e.g., for sample prep or biochemical assays), choose a defined buffer system (phosphate, Tris, HEPES) appropriate to your pH range and ionic strength requirements.

Green Alternatives

While “green alternatives” typically refer to solvent or reagent substitutions, for culture media the sustainability focus shifts to preparation efficiency, waste reduction, and energy use. Consider the following (general guidance):

• Ready‑to‑use sterile plates or broths vs. dehydrated powders:
  • Pros (ready‑to‑use): Eliminates autoclave energy and water use; consistent plate quality; reduces chemical handling and dust exposure.
  • Cons: Higher packaging and transport footprint; shorter shelf life once produced; less flexibility in custom volumes.
• Alternative selective media (literature examples): Mannitol Salt Agar (MSA) or Baird‑Parker Agar can serve related purposes in staphylococcal work, with differing selectivity and differentiation mechanisms. Selection should be driven by your research question, not “greenness.”
• Process optimizations:
  • Prepare only the volume required to minimize discard of expired media.
  • Use energy‑efficient autoclaves and run full loads.
  • Choose reusable glassware over single‑use plastics where biosafety permits.

Trade‑offs

• Dehydrated media enable on‑demand preparation (lower spoilage) but require heat sterilization. Ready‑to‑use formats reduce lab energy but increase transportation mass/volume.

Note: Composition‑specific environmental metrics are not available for this item; consult institutional sustainability guidelines for microbiology labs.

Pharmaceutical Uses

Not applicable as a pharmaceutical excipient or therapeutic. This product is intended strictly for research use as a microbiological growth medium (per Product Data). In pharmaceutical and biomanufacturing research contexts, selective media like this may be used in environmental monitoring studies, cleanliness assessments, or upstream screening workflows—always under research/quality investigation protocols and not as a component of patient‑facing products.

Regulatory note

• No pharmacopeial monograph is implied. Any use in GMP or QC settings requires internal validation and supplier documentation (CoA/Spec Sheet, SDS, and performance data) for the specific lot.

Physical Properties

Because this is a dehydrated, multi‑component medium, single‑compound physical constants do not apply. Where relevant, typical values are provided as literature guidance and must not be interpreted as specifications for this item.

Item-specific (from Product Data)

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Storage conditions: Room temperature (as provided)
• Shipping: Normal (ambient)

General/literature characteristics for dehydrated microbiological media (informational)

• Physical form: Free‑flowing, hygroscopic powder or granules (literature)
• Color (dehydrated): Tan to beige, may vary by lot and supplier (literature)
• Solubility: Designed to dissolve/disperse in purified water; final clarity may range from slightly opalescent to clear depending on formulation (literature)
• pH of prepared medium: Typically formulation‑specific; commonly near neutral for Staphylococcus‑selective agars/broths, but exact value varies by brand (literature). Not specified for this item; refer to CoA/Spec Sheet.
• Preparation temperature: Dissolution aided by heating; sterilization typically by autoclaving at 121 °C (literature; see Application Protocols).

Do not use the above as product specifications. For lot‑specific reconstitution factors, pH, and performance criteria, consult the product label and CoA/Specification Sheet.

Quality and Grades

Item-specific

• Grade: BioReagent (from Product Data)

What BioReagent generally implies (general guidance)

• Intended for biological and microbiological research applications where consistent performance and low background contamination are important.
• Typically produced with controlled raw materials and documented manufacturing traceability; however, it is not a clinical/diagnostic grade unless explicitly stated by the manufacturer.

How to interpret quality for culture media

• For dehydrated media, fitness for purpose is demonstrated by growth promotion and selectivity testing against defined control strains. Lot‑specific performance parameters (e.g., recovery rates, selectivity, typical colony morphology) are established by the supplier.
• UV cutoff, trace metals, or chromatographic absorbance values are not generally applicable metrics for culture media and are not provided here.

What is specified vs. where to look

• Composition, reconstitution factor (g/L), target pH after sterilization, and performance claims are formulation‑specific: Not specified for this item; refer to CoA/Spec Sheet.
• If your workflow requires additional certifications (e.g., ISO-compliant QC, animal‑component‑free, BSE/TSE statements), consult the lot documentation or contact Technical Support with SKU S1528434.

Reaction and Applications

This product is a microbiological culture medium; it is not used in synthetic organic reactions. Its applications are biological/culture‑based.

Research applications (literature guidance)

• Selective cultivation and presumptive differentiation of Staphylococcus species from mixed samples based on tolerance to elevated ionic/osmotic conditions and other selective agents typical of Staphylococcus-selective formulations (exact composition not specified for this item).
• Enumeration and isolation workflows in food, environmental, or industrial microbiology research, quality investigations, strain banking, and phenotypic studies—strictly for research use as stated.

Practical notes

• Typical incubation regimes for staphylococci: 35–37 °C for 24–48 h (literature). Longer incubation may enhance recovery of stressed cells at the expense of selectivity.
• Use appropriate positive and negative control strains to verify performance with each prepared batch.
• Follow aseptic technique; streak for isolation to obtain discrete colonies suitable for subsequent confirmatory tests (e.g., Gram stain, catalase test, coagulase test—performed outside the scope of this product and per institutional SOPs).

Limitations

• Phenotypic selectivity is not absolute; other salt‑tolerant or formulation‑tolerant organisms may grow. Always confirm identity with orthogonal tests (biochemical, molecular) in research settings.

Note: Because composition and reconstitution factors are not specified for this catalog entry, consult the CoA/Spec Sheet for precise preparation instructions and performance characteristics.

Reaction Conditions

Chemical reaction conditions are not applicable. The following provides general, literature‑based preparation conditions for dehydrated culture media; they are offered as guidance only and are not specifications for this product.

General preparation (literature)

• Reconstitution: Suspend the indicated mass of dehydrated medium in purified water to the final volume specified on the label (not specified for this item; refer to CoA/Spec Sheet). Allow components to fully wet before heating.
• Dissolution: Heat with frequent agitation until fully dissolved; avoid prolonged boiling prior to sterilization to limit degradation of heat‑sensitive components.
• Sterilization: Autoclave at 121 °C for 15 minutes at 15 psi (standard microbiology practice), unless the formulation contains heat‑labile supplements that should be filter‑sterilized and added aseptically after cooling.
• Agar media: If solid medium is required, pour plates at 45–50 °C to minimize condensation and preserve selectivity.
• Incubation: Typical for staphylococci is 35–37 °C for 24–48 h (literature). Adjust based on experimental design.

Quality control (literature)

• Include positive/negative control strains to verify growth‑promotion and selectivity with each lot and preparation.

Always follow the precise instructions on the product label/CoA for this SKU.

Safety and Handling

GHS/SDS

• Signal word, hazard statements, pictograms, and classification: Not specified for this item; refer to SDS.

General safety considerations for dehydrated culture media (literature/good practice)

• Avoid creating dust; fine particulates may cause mechanical irritation to eyes/respiratory tract. Use in a well‑ventilated area or a biological safety cabinet when appropriate for the organism handled.
• Wear appropriate PPE: lab coat, safety glasses, and nitrile gloves. Use a dust mask/respirator if airborne dust cannot be controlled (per institutional risk assessment).
• This medium is non‑sterile; once hydrated it readily supports microbial growth. Treat all used media and cultures as biohazardous. Decontaminate by autoclaving (e.g., 121 °C for ≥15–30 min) or using validated disinfectants before disposal.
• Incompatibilities: Store away from strong oxidizers and moisture. Hygroscopic—keep container tightly closed with desiccant where possible.
• First aid (overview; consult SDS): If inhaled, move to fresh air; if eye contact occurs, rinse cautiously with water for several minutes; if skin contact occurs, wash with soap and water; if ingested, rinse mouth and seek medical attention if symptoms persist.

Handling tips

• Use clean glassware and high‑purity water to minimize background growth inhibition or precipitation.
• Label all media with preparation date and lot for traceability.
• Always defer to the product SDS and your institutional biosafety procedures for authoritative guidance.

Solvent Selection

Primary solvent

• Water (purified or deionized) is the intended solvent for reconstituting dehydrated media. Use water quality consistent with microbiological media preparation (e.g., ASTM Type II or better), free of inhibitory levels of chlorine or heavy metals.

Polarity and miscibility

• Complex media components (peptones, salts) are water‑soluble or water‑dispersible. Organic solvents are not appropriate for reconstitution.

Practical guidance

• Warm water (50–60 °C) and gentle stirring facilitate dissolution. Avoid boiling before complete wetting to prevent localized caramelization of carbohydrates (if present in the formulation, not specified for this item).
• Adjust the final volume only after full dissolution; verify pH if the formulation specifies a target value (not specified here; see CoA/Spec Sheet).

Comparison (literature context)

• Tap water can introduce inhibitory ions or residual disinfectants—prefer purified water.
• Buffered diluents (e.g., phosphate buffers) should not replace water unless explicitly called for by the formulation, as buffering capacity can alter selectivity.

Post‑reconstitution

• Sterilize by autoclaving unless using a sterile, ready‑to‑use format. Filter sterilization is generally unsuitable for complex particulate media and agars.

Storage and Reconstitution

Item-specific storage

• Storage conditions (dehydrated powder): Room temperature (per Product Data). Protect from moisture and direct sunlight. Keep container tightly closed; use desiccant if available.
• Shipped: Normal ambient conditions (per Product Data).

Shelf life and stability

• Lot‑specific expiry dating and any special stabilizers: Not specified for this item; refer to CoA/Spec Sheet.

Reconstitution

• Solvent: Purified/deionized water.
• Reconstitution factor (g/L), target pH, and any supplements: Not specified for this item; refer to CoA/Spec Sheet.
• Sterilization: Autoclave at 121 °C for 15 minutes unless the formulation or supplements are heat‑labile (literature guidance).

Prepared media handling (general)

• Store prepared broth or plates at 2–8 °C, protected from light and desiccation. Typical in‑use shelf life ranges from several days to a few weeks depending on formulation and storage; confirm with internal QC (not specified for this item).
• Avoid repeated temperature fluctuations and prolonged light exposure that may degrade indicators (if present; formulation not specified).

Freeze–thaw

• Do not freeze dehydrated medium. Freezing prepared agar plates is not recommended due to cracking and loss of performance.

Always follow the label and CoA/Spec Sheet for lot‑specific instructions for SKU S1528434.

Structure and Identity

This product is a complex, dehydrated microbiological culture medium designed for the selective cultivation of staphylococci. It is a formulated mixture, not a single, well-defined chemical compound.

• Compound type: Dehydrated culture medium (microbiology); multi‑component formulation
• Molecular formula: Not applicable to mixtures
• Molecular weight: Not applicable to mixtures
• SMILES / InChI / InChIKey: Not applicable to mixtures
• Structural features: Not applicable; the product consists of a blend of organic and inorganic nutrients, salts, and selective/differential agents (exact composition not specified for this item).

2D structure description

• Not applicable. For complex media, identity is defined by formulation and performance characteristics rather than a discrete molecular structure.

Item-specific identity notes (from Product Data)

• SKU: S1528434
• Product Name: Staphylococcus Medium 110
• Grade: BioReagent
• Category: Life Science research reagents (microbiology medium)
• Research Use: For research use only.

Synthetic Utility

Not applicable. Staphylococcus Medium 110 is a biological culture medium and is not employed as a reagent, catalyst, or solvent in chemical synthesis. For synthetic workflows, refer to reagents and solvents designed for chemical transformations. See other catalog categories for building blocks, catalysts, and anhydrous solvents.

Target Specificity

This section is not applicable. No antibody or biomolecular targeting information (antigen, epitope, clone, isotype, species reactivity) is provided for this product. Staphylococcus Medium 110 is a selective culture medium rather than an affinity reagent. For immunochemical targeting details, consult products specifically designed as antibodies, probes, or binding ligands.