Trypan Blue - Biological stain , CAS No.72-57-1
In stock
SKU
T108745
Product Introduction
Trypan Blue is an azo based, hydrophilic, tetrasulfonated blue acid dye widely utilized for assessing cell viability. As an easy method to determining the viability of cells, Trypan Blue will stain dead cells with permeable membranes blue, while the dye is excluded by most living cells and their intact membranes thereby allowing visual determination of living versus dead cells. Although Trypan Blue is predominately used for assessing cell viability of cultured cells, other applications for this dye have been reported. In rat abdominal organ slices Trypan Blue has been utilized to determine cell viability as well as islet cell cluster cell viability from caprine pancreatic samples.
A viability dye for quantifying live versus dead cells
The trypan blue dye is an azo dye, derived from toluidine. It is soluble in water and has a molecular weight of 961 g/mol.
Application
Trypan blue has been used:
in cell viability assay
to determine the number of living and necrotic cells by staining with 0.2% trypan blue solution.
to stain plant tissue in order to to visualize cell death
More Information
| CAS | 72-57-1 |
|---|---|
| Synonyms | C.I. 23850 | Centraline Blue 3B | Bleue diretto 3B | Modr Prima 14 [Czech] | Sodium ditolyl-diazobis-8-amino-1-naphthol-3,6-disulfonate | Benzanil Blue R | Congoblau 3B | Dianilblau H3G | TRYPAN BLUE [MART.] | Diaminblau 3B | NCGC00167554-01 | TRYPAN BLUE |
| Specifications & Purity | Biological stain |
| Storage | Desiccated,Room temperature |
| Shipped In | Normal |
| Product Description | Product Introduction Trypan Blue is an azo based, hydrophilic, tetrasulfonated blue acid dye widely utilized for assessing cell viability. As an easy method to determining the viability of cells, Trypan Blue will stain dead cells with permeable membranes blue, while the dye is excluded by most living cells and their intact membranes thereby allowing visual determination of living versus dead cells. Although Trypan Blue is predominately used for assessing cell viability of cultured cells, other applications for this dye have been reported. In rat abdominal organ slices Trypan Blue has been utilized to determine cell viability as well as islet cell cluster cell viability from caprine pancreatic samples. The trypan blue dye is an azo dye, derived from toluidine. It is soluble in water and has a molecular weight of 961 g/mol. Application Trypan blue has been used: in cell viability assay to determine the number of living and necrotic cells by staining with 0.2% trypan blue solution. to stain plant tissue in order to to visualize cell death |
| Pubchem Sid | 504751057 |
| Pubchem Sid Url | https://pubchem.ncbi.nlm.nih.gov/substance/504751057 |
| Solubility | Soluble in water (10 mg/ml at 25 °C), ethanol (0.6 mg/ml), and EGME (20 mg/ml). |
| Mdl Number | MFCD00003969 |
| EC Number | 200-786-7 |
| PubChem CID | 6296 |
| Molecular Formula | C34H24N6Na4O14S4 |
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