TWEEN ® 20 - viscous liquid , CAS No.9005-64-5

CAS: 9005-64-5 Cat. No.: T104863 Formula: C₅₈H₁₁₄O₂₆(for n = 20) Molecular Weight: 1228 (for n= 20) EC Number: 500-018-3

GRADE & PURITY viscous liquid

Synonyms

POE (6) sorbitol monolaurate | Tween(R) 20 | 2-{2-[3,4-bis(2-hydroxyethoxy)oxolan-2-yl]-2-(2-hydroxyethoxy)ethoxy}ethyl dodecanoate | SCHEMBL118527 | Tween 40 (TN) | 1052273-76-3 | 2-(2-(3,4-bis(2-hydroxyethoxy)tetrahydrofuran-2-yl)-2-(2-hydroxyethoxy)eth

Storage

Room temperature

Shipped In

Normal

Overview

Tween 20 is an ethoxylated (20) sorbitan ester based on a natural fatty acid (lauric acid). This ethoxylate is highly effective at forming O/W emulsions, particularly when used in combination with its non-ethoxylated derivative, Span 20. Chemical group Nonionic Surfactants - Sorbitan Esters and Ethoxylated Sorbitan Esters Form @ 25oC Clear yellow liquid HLB 16.7 Manufacturer：Croda
Non-ionic detergent

ECO TWEEN® 20, also known as Polyoxyethylenesorbitan monolaurate, is a bio-based, nonionic detergent manufactured from a natural fatty acid. This surfactant, derived from natural sources, provides various functional advantages, such as detergency, emulsification, and wetting. Its versatility makes it well-suited for a variety of applications in biochemical and biological research, with common uses including cell lysis, protein extraction, nuclei isolation and cell fractionation. We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Green Chemistry. This product is a biobased surfactant and is aligned with the 7th principle of Green Chemistry "Use of Renewable Feedstocks" and the 10th principle "Design for Degradation".
TWEEN 20 is a polyoxyethylene sorbitol ester that belongs to the polysorbate family. It is a nonionic detergent having a molecular weight of 1,225 daltons, assuming 20 ethylene oxide units, 1 sorbitol, and 1 lauric acid as the primary fatty acid. The ethylene oxide subunits are responsible for the hydrophilic nature of the surfactant, while the hydrocarbon chains provide the hydrophobic environment. Sorbitol forms the backbone ring to which the ethylene oxide polymers are attached.

Product Application
TWEEN 20 has been used as a component of:
washing buffer and blocking buffer in Western blotting
blocking buffer in immunohistochemistry
washing buffer in ELISA (enzyme linked immunosorbent assay)
reaction mixture in PCR (polymerase chain reaction)
TWEEN 20 is a nonionic detergent widely used in biochemical applications. It has been used as an emulsifying agent for the preparation of stable oil-in-water emulsions. TWEEN 20 has been used in pre-extraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins).
TWEEN 20 has been used as a blocking agent for membrane based immunoassays at a typical concentration of 0.05%. TWEEN 20 can be used for lysing mammalian cells at a concentration of 0.005 to 0.5%.

Specifications

Synonyms

POE (6) sorbitol monolaurate | Tween(R) 20 | 2-{2-[3,4-bis(2-hydroxyethoxy)oxolan-2-yl]-2-(2-hydroxyethoxy)ethoxy}ethyl dodecanoate | SCHEMBL118527 | Tween 40 (TN) | 1052273-76-3 | 2-(2-(3,4-bis(2-hydroxyethoxy)tetrahydrofuran-2-yl)-2-(2-hydroxyethoxy)eth

Specifications & Purity

viscous liquid

Legal Information

TWEEN is a registered trademark of Croda International PLC

Storage

Room temperature

Shipped In

Normal

Names and Identifiers

Pubchem Sid

508812883

Pubchem Sid Url

https://pubchem.ncbi.nlm.nih.gov/substance/508812883

Canonical Smiles

CCCCCCCCCCCC(=O)OCCOCC(C1C(C(CO1)OCCO)OCCO)OCCO

IUPAC Name

2-[2-[3,4-bis(2-hydroxyethoxy)oxolan-2-yl]-2-(2-hydroxyethoxy)ethoxy]ethyl dodecanoate

InChIKey

HMFKFHLTUCJZJO-UHFFFAOYSA-N

INCHI

1S/C26H50O10/c1-2-3-4-5-6-7-8-9-10-11-24(30)34-19-18-31-20-22(32-15-12-27)26-25(35-17-14-29)23(21-36-26)33-16-13-28/h22-23,25-29H,2-21H2,1H3

Isomeric SMILES

CCCCCCCCCCCC(=O)OCCOCC(C1C(C(CO1)OCCO)OCCO)OCCO

WGK Germany

1

RTECS

TR7400000

Molecular Weight

1228 (for n= 20)

Taxonomic Classification

Taxonomy Tree

• Kingdom Organic compounds
  • Superclass Lipids and lipid-like molecules
    • Class Fatty Acyls
      • Subclass Fatty acid esters

• Kingdom: Organic compounds
• Superclass: Lipids and lipid-like molecules
• Class: Fatty Acyls
• Subclass: Fatty acid esters
• Intermediate Tree Nodes: Not available
• Direct Parent: Fatty acid esters
• Alternative Parents: Tetrahydrofurans Carboxylic acid esters Oxacyclic compounds Monocarboxylic acids and derivatives Dialkyl ethers Primary alcohols Organic oxides Hydrocarbon derivatives Carbonyl compounds
• Molecular Framework: Aliphatic heteromonocyclic compounds
• Substituents: Fatty acid ester - Tetrahydrofuran - Carboxylic acid ester - Carboxylic acid derivative - Dialkyl ether - Ether - Monocarboxylic acid or derivatives - Organoheterocyclic compound - Oxacycle - Organic oxide - Carbonyl group - Alcohol - Organooxygen compound - Primary alcohol - Hydrocarbon derivative - Organic oxygen compound - Aliphatic heteromonocyclic compound
• Description: This compound belongs to the class of organic compounds known as fatty acid esters. These are carboxylic ester derivatives of a fatty acid.
• External Descriptors: a small molecule

Data sources

1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.

DOI: 10.1186/s13321-016-0174-y

Mechanisms of Action

Chemical and Physical Properties

• Solubility: Soluble in water; Soluble in Dioxane,Ethanol,Methanol
• Sensitivity: Moisture sensitive.Heat sensitive
• x_refractive_index: 1.4685-1.4715
• x_flash_point_drop_centigrade: >149℃
• x_boil_point_char: 100°C
• x_melt_point_char: -14 °C
• Molecular Weight: 522.700 g/mol
• XLogP3: 2.500
• Hydrogen Bond Donor Count: 3
• Hydrogen Bond Acceptor Count: 10
• Rotatable Bond Count: 26
• Exact Mass: 522.34 Da
• Monoisotopic Mass: 522.34 Da
• Topological Polar Surface Area: 133.000 Ų
• Heavy Atom Count: 36
• Formal Charge: 0
• Complexity: 507.000
• Isotope Atom Count: 0
• Defined Atom Stereocenter Count: 0
• Undefined Atom Stereocenter Count: 4
• Defined Bond Stereocenter Count: 0
• Undefined Bond Stereocenter Count: 0
• The total count of all stereochemical bonds: 0
• Covalently-Bonded Unit Count: 1