Universal Antibody Dilution Buffer - Suitable for Immunofluorescence(IF), BioReagent, ready-to-use, Suitable for molecular biology, sterile, for western blot, Suitable for Immunohistochemistry(IHC)

Cat. No.: W666033
AVAILABLE TO ORDER
GRADE & PURITY Suitable for molecular biology ? Molecular-biology grade — free of nucleases and contaminants that degrade DNA/RNA. Use in cloning, PCR, and nucleic-acid work needing clean reagents. BioReagent ? BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility. Ready-to-use ? Ready-to-use — supplied pre-formulated at working concentration, no prep needed. Use to save time and reduce pipetting/dilution errors. Sterile ? Sterile grade — processed and verified free of viable microorganisms. Use directly in aseptic procedures and cell culture without further sterilization. for Western blot ? Western-blot grade — suited to protein transfer and immunodetection. Use in Western workflows where reagent purity keeps background low. Suitable for Immunohistochemistry(IHC) ? IHC grade — validated for immunohistochemistry on tissue sections. Use to detect and localize antigens in fixed tissue. Suitable for Immunofluorescence(IF) ? IF grade — validated for immunofluorescence with low background staining. Use to localize targets in cells/tissue by fluorescence microscopy.
Synonyms
Antibody Dilution Solution | Antibody Dilution Buffer | Universal antibody diluent | Primary and Secondary antibody diluent | Diluted solution of primary and secondary antibodies
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
 ·  off list, applied to all prices below.
Size
Status
Price
Qty
100ml
W666033-100ml
2

$36.90

$54.90
Save $18.00 (32.79%)
500ml
W666033-500ml
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.

$134.90

$169.90
Save $35.00 (20.60%)
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Why this grade

Suitable for Immunofluorescence(IF), BioReagent, ready-to-use, Suitable for molecular biology, sterile, for western blot, Suitable for Immunohistochemistry(IHC) BioReagent,for Western blot,Ready-to-use,Sterile,Suitable for Immunofluorescence(IF),Suitable for Immunohistochemistry(IHC),Suitable for molecular biology for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

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Storage & shipping

Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

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Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 3 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

Product Introduction:

The Universal antibody diluent is optimized through repeated optimization of an appropriate amount of BSA, buffer, detergent, polymer, and non competitive protein to form a protective effect on the antibody protein and improve the specific binding of the antibody to the target molecule. At the same time, it is also beneficial to prevent non-specific binding between antibodies and membranes or non target proteins, and effectively improve the stable storage time of diluted primary antibodies.

Precautions

1. This product contains BSA. If bacterial growth occurs, please stop using it.

2. For your safety and health, please wear lab clothes and disposable gloves when operating.

Instructions for Use:

Dilute the corresponding primary and secondary antibodies with Universal antibody diluent according to the recommended conditions in the antibody manual. For example, dilute at a ratio of 1:1000 by adding 1 µL of antibody to 1000 µ L of Universal antibody dilution solution.



Specifications

Synonyms
Antibody Dilution Solution | Antibody Dilution Buffer | Universal antibody diluent | Primary and Secondary antibody diluent | Diluted solution of primary and secondary antibodies
Specifications & Purity
Suitable for Immunofluorescence(IF), BioReagent, ready-to-use, Suitable for molecular biology, sterile, for western blot, Suitable for Immunohistochemistry(IHC)
Stability And Storage
Store at 2-8℃ for 12 months; Store at -20℃ for 24 months. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Storage
Store at -20°C, Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
This product requires cold chain shipping. Ground and other economy services are not available.
Grade
BioReagent, for Western blot, Ready-to-use, Sterile, Suitable for Immunofluorescence(IF), Suitable for Immunohistochemistry(IHC), Suitable for molecular biology
Names and Identifiers
PH7.0~7.6

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot NumberCertificate TypeDateItem
ZJ26F0535125Certificate of AnalysisMay 09, 2026 W666033
ZJ26F0535124Certificate of AnalysisMay 09, 2026 W666033
Documents & Articles
Citations of This Product
References
1. Jin Cui, Gongzheng Wang, Li Xian Yip, Mengzhen Dong, Mengyao Mu, Liya Tian, Yuan Gao, Qing Fan, Qiang Zhu, Xinya Zhao, Xueli Xu, David Tai Leong, Xiao Sun.  (2024)  Enhanced Ferritin-Manganese Interaction by Nanoplatinum Growth Enabling Liver Fibrosis 3D Magnetic Resonance Visualization and Synergistic Therapy with Real-Time Monitoring.  ADVANCED FUNCTIONAL MATERIALS,      [PMID:] [10.1002/adfm.202410748]
2. Yuan Gao, Mengyao Mu, Yiju Wei, Bowen Yan, Hui Liu, Kai Guo, Mengmeng Zhang, Xiaohui Dai, Xiao Sun, David Tai Leong.  (2024)  Novel ultrathin ferrous sulfide nanosheets: Towards replacing black phosphorus in anticancer nanotheranostics.  Bioactive Materials,      [PMID:40115876] [10.1016/j.bioactmat.2024.09.035]
3. Guiqi Ma, Xinyu Zhang, Kunlong Zhao, Shuxuan Zhang, Ke Ren, Mengyao Mu, Chenyu Wang, Ximing Wang, Hui Liu, Jian Dong, Xiao Sun.  (2024)  Polydopamine Nanostructure-Enhanced Water Interaction with pH-Responsive Manganese Sulfide Nanoclusters for Tumor Magnetic Resonance Contrast Enhancement and Synergistic Ferroptosis–Photothermal Therapy.  ACS Nano,      [PMID:38251846] [10.1021/acsnano.3c10249]
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