This product is a 10×BU buffer at pH 7.0 and has been filter-sterilized. It can be used to prepare X-gal chromogenic medium for detecting the LacZ reporter gene in the EGY48 yeast one-hybrid system.
10×BU solution (pH = 7.0, sterile) provides a buffering environment for yeast X-gal chromogenic experiments, making yeast colonies develop clearer and more persistent color.
Instructions for Use (using a 500 mL system as an example):
Preheat a 20% galactose solution (sterile), a raffinose solution (20%, filter-sterilized) (Aladdin Catalog No.: R1524582), and the 10×BU solution (pH = 7.0, sterile) at 55 ℃ in advance. Cool the sterilized 500 mL medium to 55 ℃, then sequentially add 50 mL of 20% galactose solution (sterile), 25 mL of raffinose solution (20%, filter-sterilized), 50 mL of 10×BU solution (pH = 7.0, sterile), and 2 mL of X-Gal solution (20 mg/mL) (Aladdin Catalog No.: X1520260). Mix well and pour plates. After solidification, store at 2–8 ℃ protected from light for subsequent use.
Precautions:
1.Pay attention to aseptic operation to avoid microbial contamination.
2.This product is for research use only. Do not use it for pharmaceutical, clinical diagnostic or therapeutic, food, or cosmetic purposes.
3.For your safety and health, please wear a laboratory coat and disposable gloves and a mask during operation.
