155H1 , CAS No.H1423782

CAS: H1423782 Cat. No.: H1423782 Formula: C₇₉H₁₂₀FN₁₉O₂₃S Peso molecolare: 1754.98

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Panoramica

155H1 (Compound 11) is a stapled peptide, that covalently binds hMcl1 (172-323) with IC 50 of 18 nM.

Specifications

Condizioni di conservazione di stoccaggio

Store at -20°C

Spedito in

Ice chest + Ice pads

Questo prodotto richiede spedizione a catena fredda. I servizi di terra e altri servizi economici non sono disponibili.

Tipo di azione

INHIBITOR

Nomi e identificatori

• Peso molecolare: 1754.98

Application Protocols

No vendor‑validated application protocols are provided specifically for 155H1. The following are general best practices for small‑molecule screening compounds (not item‑specific):

Preparing stock solutions:

• Warm vial to room temperature in a desiccator. Dissolve in anhydrous DMSO to 10–50 mM. Vortex and, if clear, optionally 0.22 µm PTFE filter. Record exact concentration.

Assay dilution:

• Perform serial dilutions in DMSO to create an intermediate plate (e.g., 10‑point 3‑fold). Back‑dilute into assay buffer or media to achieve final ≤0.1–0.5% DMSO.

Plate handling:

• Use low‑bind tips/plates; keep plates sealed to limit evaporation. Equilibrate plates to room temperature before opening to minimize condensation.

Interference controls:

• Include vehicle controls, counter‑screens for fluorescence/absorbance interference, and detergent controls to detect aggregation.

Data integrity:

• Record SKU (H1423782), lot, concentration, and storage history. Archive raw data with plate maps.

Note: For this item, any specific protocol parameters (e.g., wavelengths, target concentrations) are not specified; refer to CoA/Spec Sheet and your internal SOPs.

Biological Roles

No biological roles, pathways, or target interactions are provided in the Product Data for 155H1.

• Target, mechanism, and pathway: Not specified for this item; refer to CoA/Spec Sheet.
• As a coded screening library compound, any biological activity must be established empirically by the end user. Do not assume pharmacology or selectivity.

General considerations for screening (not item‑specific):

• Validate compound integrity (purity, identity) before biological testing to avoid artifactual results.
• Control for aggregators and PAINS behavior using orthogonal assays and detergent controls when necessary.
• Track vehicle effects (DMSO), solubility limits, and potential fluorescence/absorbance interference in optical readouts.

Research use only: As stated by the manufacturer, this product is for research use only and is not intended for human or veterinary applications.

Buffer Applications

Buffering systems are not typically applicable to an undefined small‑molecule library member. No item‑specific buffer role is provided.

Practical notes for assay buffers with small‑molecule stocks (general guidance):

• Maintain final co‑solvent ≤0.1–0.5% v/v DMSO or ethanol to minimize vehicle effects on enzymes/cells.
• For solubility, add compound last to a pre‑made buffer (e.g., PBS, HEPES, Tris) and mix thoroughly; consider gentle heating (<37 °C) or sonication if compatible.
• Include detergent (e.g., 0.01–0.05% Tween‑20) to mitigate non‑specific adsorption/aggregation when appropriate to the assay.

Item‑specific buffer compatibility, pKa, and ionic strength preferences: Not specified for this item; refer to CoA/Spec Sheet.

Green Alternatives

Because the structure and specific use case of 155H1 are undisclosed, green‑chemistry considerations focus on solvent and process choices during handling and assay preparation (general guidance).

• Prefer water‑based systems where feasible, using minimal co‑solvent (e.g., ≤0.1–0.5% DMSO or ethanol in assays) to reduce solvent burden.
• When organic stocks are needed, consider the following tradeoffs (literature/general):

Comparison (general, not item‑specific):

• DMSO vs DMF: DMSO is less volatile and generally less hazardous than DMF; DMSO often preferred for stocks if compatible.
• Ethanol vs methanol: Ethanol is less toxic and more sustainable; use ethanol when solubility allows.
• Acetonitrile vs dichloromethane/THF: For sample prep and LC, acetonitrile is preferred over halogenated solvents; avoid chlorinated solvents unless strictly necessary.

Operational practices:

• Use miniaturized assay volumes and sealed plates to lower solvent use and emissions.
• Implement solvent recovery where applicable and segregate waste streams for compliant disposal.

Item‑specific environmental metrics (E‑factor, LCA, etc.): Not specified for this item; refer to CoA/Spec Sheet.

Pharmaceutical Uses

No pharmaceutical or excipient uses are provided or implied for 155H1.

• Pharmacopeial status (USP/EP/JP): Not specified for this item; refer to CoA/Spec Sheet.
• Typical role: This product is supplied for research use only, commonly as a screening compound in discovery settings. It is not manufactured to GMP standards and is not intended for human or veterinary use, drug substance, or excipient applications.

General formulation notes for research assays (not item‑specific):

• For in vitro or ex vivo studies, prepare DMSO or ethanol stocks and dilute into compatible media, keeping vehicle levels minimal and consistent across controls.
• Document lot numbers and storage history to ensure reproducibility in discovery workflows.

Any clinical, diagnostic, or therapeutic claims are outside scope and are not supported for this product.

Physical Properties

Item‑specific physical constants are not provided in the Product Data.

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Melting point: Not specified for this item; refer to CoA/Spec Sheet.
• Boiling point: Not specified for this item; refer to CoA/Spec Sheet.
• Density: Not specified for this item; refer to CoA/Spec Sheet.
• Refractive index: Not specified for this item; refer to CoA/Spec Sheet.
• LogP, pKa(s): Not specified for this item; refer to CoA/Spec Sheet.
• Solubility: Not specified for this item; refer to CoA/Spec Sheet.

General laboratory guidance (literature/practice — not item‑specific):

• Screening library compounds are commonly formulated as 10–50 mM DMSO stocks due to broad solubility coverage. If DMSO is unsuitable, try anhydrous DMF, ethanol, or aqueous buffers with co‑solvent (≤1–2% v/v in final assay).
• For unknown solids, a small‑scale solubility screen at room temperature in DMSO, MeOH, EtOH, DMF, acetonitrile, and PBS (with co‑solvent) helps establish handling conditions.
• If crystalline, DSC or melting point apparatus can establish a preliminary MP (general lab characterization). Avoid prolonged heating of unknown compounds.

Note: Treat all practical values above as general literature guidance only; for this specific item, consult the CoA/Spec Sheet and SDS for authoritative data.

Quality and Grades

• Grade/Purity (item‑specific): Not specified for this item; refer to CoA/Spec Sheet.

Context for professional users (general information about library compounds):

• Screening library members are commonly supplied at analytical or research grade, often accompanied by HPLC purity and identity confirmation (NMR/HRMS). However, exact specifications are vendor‑ and lot‑dependent. Do not assume a purity threshold without the CoA.
• UV cutoff, residual solvent profile, water content (KF), metal content, and stabilizers (if any) can impact sensitive assays. For 155H1, these parameters are not specified for this item; refer to CoA/Spec Sheet.
• If using in sensitive biophysical assays (SPR, ITC) or photoactive assays, request absorbance spectra and impurity profiles to ensure compatibility.
• For hit follow‑up or SAR, consider ordering fresh lots and verifying identity/purity in‑house (orthogonal methods such as HPLC‑UV/ELSD, 1H/13C NMR, HRMS).

Documentation and traceability:

• Reference the SKU (H1423782) and product name (155H1) in reports and ELNs. Retain CoA/SDS for the received lot number to ensure reproducibility and regulatory compliance within your institution.

Reaction and Applications

This product is offered as a screening library small molecule rather than a defined synthetic reagent; there are no item‑specific reaction applications in the Product Data.

• Intended use: Research screening and discovery workflows (e.g., biochemical, biophysical, or cell‑based assays), subject to internal qualification of purity and identity. No target or mechanism is provided.
• Synthetic chemistry usage: Without structural disclosure, using 155H1 as a reagent/building block is not typical. If chemistry on this scaffold is desired (e.g., analog synthesis), request structural information and CoA first.

General practical tips for small‑molecule assay applications (not item‑specific):

• Prepare a concentrated DMSO master stock (e.g., 10–50 mM), filter through 0.22 µm PTFE if solution is clear and stable.
• Verify concentration by weight‑in and volume‑up or by quantitative NMR if the structure becomes available.
• Avoid freeze–thaw cycles; aliquot and store at −20 °C.
• Record vehicle controls carefully (typical final DMSO ≤0.1–0.5% v/v in bioassays).

Note: Any biological activity claims, targets, or reaction roles are not established for this item and should not be inferred in the absence of disclosed structure.

Reaction Conditions

No reaction conditions are provided for 155H1, and without structural information, synthetic or transformation conditions cannot be recommended.

General guidance for handling unknown small molecules in method development (not item‑specific):

• Begin with analytical characterization to confirm integrity (HPLC, LC/MS).
• For stability assessments, test aliquots under thermal (room temp vs 37 °C), oxidative (air vs inert), and pH stress (acidic/neutral/basic buffers) to map handling constraints.
• If chemical modification is planned, perform microscale scouting reactions and monitor by LC/MS to avoid material loss.

Item‑specific solvents, temperatures, catalysts, and yields: Not specified for this item; refer to CoA/Spec Sheet.

Safety and Handling

Hazard and GHS data are not provided in the Product Data for 155H1.

• Signal word: Not specified for this item; refer to SDS.
• GHS classification: Not specified for this item; refer to SDS.
• H‑statements/Pictograms: Not specified for this item; refer to SDS.

General safety guidance (good laboratory practice for unknown small molecules):

• Handle as potentially hazardous. Wear lab coat, safety glasses, and appropriate chemical‑resistant gloves (e.g., nitrile). Work in a certified fume hood.
• Avoid inhalation of dust/aerosols and contact with skin/eyes. Prevent ingestion. Wash thoroughly after handling.
• Storage incompatibilities: Until structure is known, avoid strong oxidizers/reductants, strong acids/bases, and moisture. Keep container tightly closed under inert headspace if possible.
• Thermal/light sensitivity: Unknown; store as supplied at −20 °C and protect from light until more data are available.
• First aid (overview; consult SDS): If inhaled—move to fresh air; if on skin—wash with soap/water; if in eyes—rinse cautiously with water for several minutes; if ingested—rinse mouth, seek medical advice. Provide SDS to medical personnel.
• Spill response: Avoid dust. Absorb with inert material; collect for disposal per institutional and local regulations.

Always consult the official SDS for 155H1 for definitive hazard, exposure limits, and emergency procedures.

Solvent Selection

Item‑specific solvent compatibility is not provided. Selection should follow a pragmatic screening approach for small molecules with unknown polarity.

Primary strategy for assay stocks (general guidance):

• Start with anhydrous DMSO (10–50 mM). It solubilizes a wide range of chemotypes and is miscible with water for downstream dilutions.
• If DMSO is unsuitable, evaluate DMF, ethanol, methanol, or acetonitrile. For aqueous work, use co‑solvent strategies (≤0.1–0.5% DMSO v/v in final assay is common to minimize vehicle effects).

Miscibility/polarity notes (literature/general):

• DMSO and DMF: polar aprotic, water‑miscible; broad solubilization power.
• Ethanol/methanol: polar protic; good for many heterocycles; fully water‑miscible.
• Acetonitrile: polar aprotic; low viscosity; fully water‑miscible.

When to choose alternatives:

• For membrane or lipid assays, consider ethanol as vehicle due to lower viscosity and cellular tolerance (verify assay tolerance).
• For LC/MS‑coupled screens, acetonitrile stocks can reduce ion suppression versus high DMSO fractions.

Note: Because the structure of 155H1 is undisclosed, finalize solvent choice empirically and document vehicle % thoroughly. For this item, item‑specific solubility/UV cutoff are not specified; refer to CoA/Spec Sheet.

Storage and Reconstitution

Item‑specific storage conditions (from Product Data):

• Store at −20 °C.
• Shipped in an ice chest with ice pads.
• Research Use Note: For research use only.

Additional best practices (general, not item‑specific):

• Keep container tightly closed, desiccated, and protected from light. If hygroscopic or light‑sensitive behavior is suspected, add desiccant and wrap the vial in foil or use amber vials.
• Upon first use, prepare concentrated aliquots (e.g., 10–50 mM in anhydrous DMSO) to avoid repeated freeze–thaw cycles. Store aliquots at −20 °C or below in sealed, inert‑gas‑blanketed vials when possible.
• Allow frozen vials to equilibrate to room temperature before opening to prevent moisture ingress. Re‑seal promptly after use.
• Stability, shelf life, and re‑test date: Not specified for this item; refer to CoA/Spec Sheet.

Disposal: Follow institutional guidelines and local regulations for disposal of organic chemical waste and contaminated materials.

Structure and Identity

Brief overview: 155H1 (SKU H1423782) is a coded small‑molecule library member offered for research screening. Detailed structure is not disclosed in the supplied Product Data.

• Product name/identifier: 155H1
• SKU: H1423782
• CAS: H1423782 (catalog reference as provided; may not correspond to a standard CAS RN)
• Category: Small molecules and compound libraries (小分子和化合物库)

Item‑specific identifiers from Product Data:

• Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
• SMILES: Not specified for this item; refer to CoA/Spec Sheet.
• InChIKey: Not specified for this item; refer to CoA/Spec Sheet.

Structural features (general notes):

• Structural class, ring systems, functional groups, and stereochemistry are not disclosed in the Product Data. If you require chemistry‑level characterization (1H/13C NMR, HRMS, HPLC purity, and structure), please request the current CoA/Spec Sheet or reach out to Aladdin Scientific technical support.

2D structure description (general):

• Not available for this coded library member. When disclosed, a 2D depiction should detail heteroatom content, ring topology (aliphatic/aromatic), substitution pattern, and any chiral centers (absolute or racemic).

Synthetic Utility

Specific synthetic utility cannot be described because the chemical structure and functional groups of 155H1 are not disclosed in the Product Data.

General considerations if synthetic follow‑up is planned (not item‑specific):

• Obtain structural disclosure and CoA to identify handleable functional groups, potential exit vectors for SAR, and any protecting group liabilities.
• Confirm purity and identity (1H/13C NMR, HRMS, HPLC) before derivatization.
• If chiral, determine enantiomeric ratio and absolute configuration to guide asymmetric synthesis or resolution approaches.
• Map feasible transformations (e.g., cross‑coupling, amide formation, N‑ or O‑alkylation) only after functional groups are known.

Item‑specific reactivity, named reactions, and retrosynthetic disconnections: Not specified for this item; refer to CoA/Spec Sheet.

Target Specificity

No target information is supplied for 155H1 in the Product Data.

• Target/class, species reactivity, affinity/selectivity metrics: Not specified for this item; refer to CoA/Spec Sheet.

As a coded screening library compound, any target specificity must be determined experimentally by the end user using orthogonal assays and appropriate controls.