2′,7′-Dichlorofluorescein - Ph.Eur., ACS, indicator , CAS No.76-54-0

CAS: 76-54-0 Cat. No.: D433767 Formula: C20H10Cl2O5 Peso molecolare: 401.2 Beilstein Registry Number: 58009 Numero EC: 200-968-6
Disponibile su ordine
GRADE & PURITY Ph.Eur. ? European Pharmacopoeia grade — conforms to Ph.Eur. monographs across EU member states. Use for pharmaceutical work needing European compendial compliance. ACS ? ACS grade — meets American Chemical Society specifications, the analytical benchmark in the US. Use for analytical and QC work needing ACS-defined purity. Indicator ? Indicator grade — characterized dye giving a defined color change at a known transition. Use to signal pH or titration endpoints visually.
Synonyms
Dichloro(R)fluorescein | DTXSID8058798 | HY-W040143A | 2',7'-DICHLOROFLUORESCEIN [MI] | FLUORESCEIN-27 | UNII-56NQM5UZT1 | Fluorescein 27 | Spiro[isobenzofuran-1(3H),9'-[9h]xanthen]-3-one,2',7'-dichloro-3',6'-dihydroxy- | 2',7'-Dichlorofluorescein [Fluore
Storage
Room temperature
Shipped In
Normal
★
Size
Germania (EU)
USA*
Price
Qty
1g
D433767-1g
Su ordinazione · 8–12 settimane
95,36€
5g
D433767-5g
Su ordinazione · 8–12 settimane

573,49€

669,81€
Salva 96,32 € (14.38%)
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Why this grade

Ph.Eur., ACS, indicator ACS,Indicatore,Ph.Eur. for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

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Storage & shipping

Room temperature Ships Normal Check lot-specific COA for exact specifications.

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Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

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Literature proof

Cited in 13 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Specifications

Sinonimi
Dichloro(R)fluorescein | DTXSID8058798 | HY-W040143A | 2',7'-DICHLOROFLUORESCEIN [MI] | FLUORESCEIN-27 | UNII-56NQM5UZT1 | Fluorescein 27 | Spiro[isobenzofuran-1(3H),9'-[9h]xanthen]-3-one,2',7'-dichloro-3',6'-dihydroxy- | 2',7'-Dichlorofluorescein [Fluore
Specifiche e purezza
Ph.Eur., ACS, indicator
Condizioni di conservazione di stoccaggio
Room temperature
Spedito in
Normal
Grado
ACS, Indicatore, Ph.Eur.
Nomi e identificatori
Sorrisi canoniciC1=CC=C2C(=C1)C(=O)OC23C4=CC(=C(C=C4OC5=CC(=C(C=C35)Cl)O)O)Cl
IUPAC Name2',7'-dichloro-3',6'-dihydroxyspiro[2-benzofuran-3,9'-xanthene]-1-one
InChIKeyVFNKZQNIXUFLBC-UHFFFAOYSA-N
INCHI1S/C20H10Cl2O5/c21-13-5-11-17(7-15(13)23)26-18-8-16(24)14(22)6-12(18)20(11)10-4-2-1-3-9(10)19(25)27-20/h1-8,23-24H
Isomeri SMILES C1=CC=C2C(=C1)C(=O)OC23C4=CC(=C(C=C4OC5=CC(=C(C=C35)Cl)O)O)Cl
Peso molecolare 401.2
Beilstein 58009
Reaxy-Rn 58009
Reaxys-RN_link_address https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=58009&ln=

Documentazione

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Taxonomic Classification

Taxonomy Tree

KingdomOrganic compounds
SuperclassOrganoheterocyclic compounds
ClasseBenzopyrans
Subclass1-benzopyrans
Intermediate Tree Nodes Dibenzopyrans
Direct ParentXanthenes
Alternative Parents Diarylethers  Phthalides  Benzofuranones  Isobenzofurans  1-hydroxy-2-unsubstituted benzenoids  Aryl chlorides  Lactones  Carboxylic acid esters  Oxacyclic compounds  Monocarboxylic acids and derivatives  Organochlorides  Organic oxides  Hydrocarbon derivatives  
Molecular FrameworkAromatic heteropolycyclic compounds
Substituents Xanthene - Diaryl ether - Benzofuranone - Phthalide - Isobenzofuranone - Isocoumaran - Isobenzofuran - 1-hydroxy-2-unsubstituted benzenoid - Aryl chloride - Benzenoid - Aryl halide - Carboxylic acid ester - Lactone - Oxacycle - Carboxylic acid derivative - Ether - Monocarboxylic acid or derivatives - Organic oxygen compound - Organooxygen compound - Hydrocarbon derivative - Organochloride - Organic oxide - Organohalogen compound - Aromatic heteropolycyclic compound
DescrizioneThis compound belongs to the class of organic compounds known as xanthenes. These are polycyclic aromatic compounds containing a xanthene moiety, which consists of two benzene rings joined to each other by a pyran ring.
External Descriptors 2-benzofurans
Struttura 3D
Modello di struttura chimica interattiva





Meccanismi d'azione
Certificati (CoA, COO, BSE/TSE e tabella di analisi)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

1 results found

Lot NumberCertificate TypeDataOggetto
E2621145Certificate of AnalysisMay 30, 2026 D433767
Proprietà chimiche e fisiche
Punto di infiammabilità (°C)12°C
Punto di fusione (°C)280°C
Peso molecolare401.200 g/mol
XLogP34.700
Hydrogen Bond Donor Count2
Hydrogen Bond Acceptor Count5
Rotatable Bond Count0
Exact Mass399.991 Da
Monoisotopic Mass399.991 Da
Topological Polar Surface Area76.000 Ų
Heavy Atom Count27
Formal Charge0
Complexity585.000
Isotope Atom Count0
Defined Atom Stereocenter Count0
Undefined Atom Stereocenter Count0
Defined Bond Stereocenter Count0
Undefined Bond Stereocenter Count0
The total count of all stereochemical bonds0
Covalently-Bonded Unit Count1
Domande frequenti e articoli
Citations of This Product
Riferimenti
1. Miao Qiao, Xin Hua, Yongjun Yuan, Xi Cao.  (2023)  Enrichment of EPA and DHA by enzymatic ethanolysis: Effects of lipases from Thermomyces lanuginosus, Rhizomucor miehei, Rhizopus oryzae and Candida antarctica.  JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY,      [PMID:] [10.1002/aocs.12800]
2. Xiangyu Meng, Yao Li, Yiqing Liu, Runyu Zhou, Yongsheng Fu, Junmin Chen.  (2023)  Degradation of organic pollutants through activating bisulfite with lanthanum ferrite-loaded biomass carbon.  RSC Advances,  13  (35): (24819-24829).  [PMID:37608974] [10.1039/D3RA04271E]
3. Yixuan Xu, Changchang Teng, Huiping Dang, Dalong Yin, Lifeng Yan.  (2023)  Highly bright stable organic radicals encapsulated by amphiphilic polypeptide for efficient near-infrared phototheranostics.  TALANTA,      [PMID:37459788] [10.1016/j.talanta.2023.124948]
4. Ping Qi, Liangying Jia, Mengjiao Yi, Enzhi Zhao, Yihan Liu, Aixin Song, Jingcheng Hao.  (2022)  Chemiluminescent gels of G-quadruplexes in deep eutectic solvents.  COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,      [PMID:] [10.1016/j.colsurfa.2022.130319]
5. Bo Huang, Xiangmei Liu, Zhaoyang Li, Yufeng Zheng, Kelvin Wai Kwok Yeung, Zhenduo Cui, Yanqin Liang, Shengli Zhu, Shuilin Wu.  (2021)  Rapid bacteria capturing and killing by AgNPs/N-CD@ZnO hybrids strengthened photo-responsive xerogel for rapid healing of bacteria-infected wounds.  CHEMICAL ENGINEERING JOURNAL,      [PMID:] [10.1016/j.cej.2021.128805]
6. Long Deng, Qian Zou, Biao Liu, Wenhui Ye, Chengfei Zhuo, Li Chen, Ze-Yuan Deng, Ya-Wei Fan, Jing Li.  (2018)  Fatty acid positional distribution in colostrum and mature milk of women living in Inner Mongolia, North Jiangsu and Guangxi of China.  Food & Function,  9  (8): (4234-4245).  [PMID:29999510] [10.1039/C8FO00787J]
7. Mengwei Ye, Weikang Zhang, Hongwei Xu, Peiyu Xie, Luming Song, Xiaohan Sun, Yian Li, Siling Wang, Qinfu Zhao.  (2024)  Fe-doped biodegradable dendritic mesoporous silica nanoparticles for starvation therapy and photothermal-enhanced cascade catalysis in tumor therapy.  JOURNAL OF COLLOID AND INTERFACE SCIENCE,      [PMID:39213991] [10.1016/j.jcis.2024.08.172]
8. Weichen Zhao, Zhangguo Wu, Meseret Amde, Hongwei Liu, Yan Li, Quanlong Wang, Yongguang Yin, Maoyong Song, Zhiqiang Tan, Yukui Rui, Qinghua Zhang, Bruce K. Gale, Guibin Jiang.  (2025)  Real-time monitoring of culture medium- and species-specific transformation of zinc oxide nanoparticles in bacterial systems using coupled CyElFFF-ICPMS.  MICROCHEMICAL JOURNAL,      [PMID:] [10.1016/j.microc.2025.114926]
9. Xiang Zhou, Jiahao Xu, Jiayao Sun, Hao Chen, Dechun Huang, Wei Chen, Hongliang Qian.  (2025)  Magnetic nano-crosslinked lipase aggregates: Preparation and catalytic synthesis of OPO.  INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,      [PMID:40925430] [10.1016/j.ijbiomac.2025.147436]
10. Siying Che, Gaoqiong Deng, Yaoyu Qi, Xiutan Peng, Haiyan Fu, Yuanbin She.  (2025)  Ion Luminescent Micelles: A Surfactant-Mediated Double-End Signal Amplification Strategy for Portable Detection of Phoxim.  ANALYTICAL CHEMISTRY,      [PMID:40900152] [10.1021/acs.analchem.5c03514]
11. Qian Ren, Xiaoyu Zhang, Xin Zhang.  (2025)  Damage visualization and enhanced interlaminar shear strength of carbon fiber reinforced composites with Ni/PUF/PU@ER microcapsules.  COMPOSITES PART A-APPLIED SCIENCE AND MANUFACTURING,      [PMID:] [10.1016/j.compositesa.2025.109338]
12. Fuying Tu, Shengmiao Sun, Zhaoge Zhang, Zhaoxu Yan, Zhidong Zhang, Liying Zhu, Ling Jiang.  (2025)  Multifunctional FeSN nanozyme-doped hydrogel: A promising strategy for antibacterial therapy and wound repair.  Biomaterials Advances,      [PMID:41297331] [10.1016/j.bioadv.2025.214620]
13. Ren Qian, Zhang Xin.  (2026)  Self-Healing Composites with Heterogeneous Interfaces and Electromagnetic Synergy for Electromagnetic Wave Absorption.  ACS Applied Materials & Interfaces,      [PMID:42604485] [10.1021/acsami.6c12113]
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